scholarly journals Studying structure and dynamics of self-assembled peptide nanostructures using fluorescence and super resolution microscopy

2017 ◽  
Vol 53 (53) ◽  
pp. 7294-7297 ◽  
Author(s):  
Sílvia Pujals ◽  
Kai Tao ◽  
Adrià Terradellas ◽  
Ehud Gazit ◽  
Lorenzo Albertazzi
Keyword(s):  
Super Resolution ◽  
Structure And Dynamics ◽  
Self Assembled ◽  
Super Resolution Microscopy

Understanding the formation and properties of self-assembled peptide nanostructures is the basis for the design of new architectures for various applications.

Unidirectional Living Growth of Self-Assembled Protein Nanofibrils Revealed by Super-resolution Microscopy

ACS Nano ◽  
2016 ◽  
Vol 10 (5) ◽  
pp. 4973-4980 ◽  
Author(s):  
Lennart H. Beun ◽  
Lorenzo Albertazzi ◽  
Daan van der Zwaag ◽  
Renko de Vries ◽  
Martien A. Cohen Stuart
Keyword(s):  
Super Resolution ◽  
Self Assembled ◽  
Super Resolution Microscopy

scholarly journals Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy

2019 ◽  
Author(s):  
Kalina L. Tosheva ◽  
Yue Yuan ◽  
Pedro M. Pereira ◽  
Siân Culley ◽  
Ricardo Henriques
Keyword(s):  
Spatial Resolution ◽  
Super Resolution ◽  
Diffraction Limit ◽  
Live Cell ◽  
New Developments ◽  
Life And Death ◽  
Non Invasive ◽  
Structure And Dynamics ◽  
Super Resolution Microscopy ◽  
Sample Environment

Super-Resolution Microscopy enables non-invasive, molecule-specific imaging of the internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its major limitations is the requirement for high-intensity illumination, generating considerable cellular phototoxicity. This factor considerably limits the capacity for live-cell observations, particularly for extended periods of time. Here, we overview new developments in hardware, software and probe chemistry aiming to reduce phototoxicity. Additionally, we discuss how the choice of biological model and sample environment impacts the capacity for live-cell observations.

Super-Resolution Microscopy in Studying the Structure and Function of the Cell Nucleus

Acta Naturae ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 42-51
Author(s):  
S. S. Ryabichko ◽  
◽  
A. N. Ibragimov ◽  
L. A. Lebedeva ◽  
E. N. Kozlov ◽  
...  
Keyword(s):  
Cell Nucleus ◽  
Super Resolution ◽  
Structure And Function ◽  
And Function ◽  
Super Resolution Microscopy

Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins

2019 ◽  
Author(s):  
Jeffrey Chang ◽  
Matthew Romei ◽  
Steven Boxer
Keyword(s):  
Rational Design ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Super Resolution ◽  
Unit Cell Size ◽  
Chlorine Substituent ◽  
Gfp Chromophore ◽  
The One ◽  
Green Fluorescent ◽  
Super Resolution Microscopy

<p>Double-bond photoisomerization in molecules such as the green fluorescent protein (GFP) chromophore can occur either via a volume-demanding one-bond-flip pathway or via a volume-conserving hula-twist pathway. Understanding the factors that determine the pathway of photoisomerization would inform the rational design of photoswitchable GFPs as improved tools for super-resolution microscopy. In this communication, we reveal the photoisomerization pathway of a photoswitchable GFP, rsEGFP2, by solving crystal structures of <i>cis</i> and <i>trans</i> rsEGFP2 containing a monochlorinated chromophore. The position of the chlorine substituent in the <i>trans</i> state breaks the symmetry of the phenolate ring of the chromophore and allows us to distinguish the two pathways. Surprisingly, we find that the pathway depends on the arrangement of protein monomers within the crystal lattice: in a looser packing, the one-bond-flip occurs, whereas in a tighter packing (7% smaller unit cell size), the hula-twist occurs.</p><p> </p><p> </p><p> </p><p> </p><p> </p><p> </p> <p> </p>

Novel organic dyes for multicolor localization-based super-resolution microscopy

2015 ◽  
Vol 9 (1-2) ◽  
pp. 161-170 ◽  
Author(s):  
Martin Lehmann ◽  
Gregor Lichtner ◽  
Haider Klenz ◽  
Jan Schmoranzer
Keyword(s):  
Organic Dyes ◽  
Super Resolution ◽  
Super Resolution Microscopy

scholarly journals Bio-orthogonal Red and Far-Red Fluorogenic Probes for Wash-Free Live-Cell and Super-resolution Microscopy

2021 ◽  
Author(s):  
Philipp Werther ◽  
Klaus Yserentant ◽  
Felix Braun ◽  
Kristin Grußmayer ◽  
Vytautas Navikas ◽  
...  
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Fluorogenic Probes ◽  
Super Resolution Microscopy
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