An ultrasensitive fluorescence aptasensor for carcino-embryonic antigen detection based on fluorescence resonance energy transfer from upconversion phosphors to Au nanoparticles

2018 ◽  
Vol 10 (13) ◽  
pp. 1552-1559 ◽  
Author(s):  
Xiang-Hui Li ◽  
Wei-Ming Sun ◽  
Juan Wu ◽  
Yao Gao ◽  
Jing-Hua Chen ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Au Nanoparticles ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Antigen Detection ◽  
Upconversion Nanoparticles ◽  
Energy Donor ◽  
Embryonic Antigen ◽  
Energy Acceptor

A FRET-based aptasensor for CEA detection was constructed by using upconversion nanoparticles as the energy donor and Au nanoparticles as the energy acceptor.

Aptamer-modified sensitive nanobiosensors for the specific detection of antibiotics

2020 ◽  
Vol 8 (37) ◽  
pp. 8607-8613
Author(s):  
Ying Zhang ◽  
Bo Duan ◽  
Qing Bao ◽  
Tao Yang ◽  
Tiancheng Wei ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Upconversion Nanoparticles ◽  
Core Shell ◽  
Specific Detection ◽  
Energy Donor ◽  
Energy Acceptor

A highly selective, fluorescence resonance energy transfer (FRET) based aptasensor for enrofloxacin (ENR) detection was developed using core–shell upconversion nanoparticles as an energy donor and graphene oxide as an energy acceptor.

scholarly journals Fluorescence sensing of cyanide anions based on Au-modified upconversion nanoassemblies

The Analyst ◽  
2021 ◽  
Author(s):  
Chunning Sun ◽  
Michael Gradzielski
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Au Nanoparticles ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Upconversion Nanoparticles ◽  
Core Shell ◽  
Fluorescence Sensing ◽  
Sensitivity And Selectivity ◽  
Fluorescence Resonance

Fluorescence resonance energy transfer (FRET)-based upconversion nanoprobes were designed by assembling Au nanoparticles on core–shell-structured upconversion nanoparticles, showing excellent sensitivity and selectivity toward cyanide ions sensing.

scholarly journals Resonance energy transfer microscopy: observations of membrane-bound fluorescent probes in model membranes and in living cells.

1986 ◽  
Vol 103 (4) ◽  
pp. 1221-1234 ◽  
Author(s):  
P S Uster ◽  
R E Pagano
Keyword(s):  
Energy Transfer ◽  
Fluorescent Probes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Living Cells ◽  
Model Membranes ◽  
Energy Donor ◽  
Membrane Bound ◽  
Energy Acceptor ◽  
In Cells

A conventional fluorescence microscope was modified to observe the sites of resonance energy transfer (RET) between fluorescent probes in model membranes and in living cells. These modifications, and the parameters necessary to observe RET between membrane-bound fluorochromes, are detailed for a system that uses N-4-nitrobenzo-2-oxa-1,3-diazole (NBD) or fluorescein as the energy donor and sulforhodamine as the energy acceptor. The necessary parameters for RET in this system were first optimized using liposomes. Both quenching of the energy donor and sensitized fluorescence of the energy acceptor could be directly observed in the microscope. RET microscopy was then used in cultured fibroblasts to identify those intracellular organelles labeled by the lipid probe, N-SRh-decylamine (N-SRh-C10). This was done by observing the sites of RET in cells doubly labeled with N-SRh-C10 and an NBD-labeled lipid previously shown to label the endoplasmic reticulum, mitochondria, and nuclear envelope. RET microscopy was also used in cells treated with fluorescein-labeled Lens culinaris agglutinin and a sulforhodamine derivative of phosphatidylcholine to examine the internalization of plasma membrane lipid and protein probes. After internalization, the fluorescent lectin resided in most, but not all of the intracellular compartments labeled by the fluorescent lipid, suggesting sorting of the membrane-bound lectin into a subset of internal compartments. We conclude that RET microscopy can co-localize different membrane-bound components at high resolution, and may be particularly useful in examining temporal and spatial changes in the distribution of fluorescent molecules in membranes of the living cell.

Sign in / Sign up

Export Citation Format

Share Document