Effect of mobile phase composition on the analysis of aggregates of antibody drug conjugates (ADCs) using size exclusion chromatography

2018 ◽  
Vol 10 (9) ◽  
pp. 938-941 ◽  
Author(s):  
Rabin Neupane ◽  
Malin Källsten ◽  
Fredrik Lehmann ◽  
Jonas Bergquist
Keyword(s):  
Phase Composition ◽  
Size Exclusion Chromatography ◽  
Mobile Phase ◽  
Size Exclusion ◽  
Mobile Phase Composition ◽  
Antibody Drug Conjugate ◽  
Aggregate Analysis ◽  
Drug Conjugates ◽  
Exclusion Chromatography ◽  
Antibody Drug

In this study, the effect of mobile phase composition for size exclusion chromatography (SEC) on antibody drug conjugate (ADC) aggregate analysis was investigated.

scholarly journals Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates

2021 ◽  
Author(s):  
Tomohiro Fujii ◽  
Calliste Reiling ◽  
Colette Quinn ◽  
Michal Kliman ◽  
Brian A. Mendelsohn ◽  
...  
Keyword(s):  
High Performance ◽  
Size Exclusion ◽  
Antibody Drug Conjugate ◽  
Scanning Calorimetry ◽  
Analytical Comparison ◽  
Drug Conjugates ◽  
Biological Studies ◽  
Comparison Results ◽  
Exclusion Chromatography ◽  
Antibody Drug

Aim: Direct analytical comparison of two major drug-linkers in the antibody-drug conjugate (ADC) field was conducted. Methods: Four different analytical methods [AlogP calculation, reverse phase (RP) high-performance liquid chromatography (HPLC; RP-HPLC), size exclusion chromatography HPLC (SEC-HPLC), and differential scanning calorimetry (DSC)] were tested for this comparison. Results: Maytansinoid-based ADCs showed less hydrophobicity than auristatin-based ADCs. Regardless of the drug-linker and drug-to-antibody ratios (DARs), the stability detected by DSC was decreased by conjugation. Conclusions: The cost and time-efficient analytical comparison described in this manuscript may be useful information for an initial characterization of ADCs prior to detailed biological studies.

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