An ultrasensitive sandwich immunoassay with a glucometer readout for portable and quantitative detection of Cronobacter sakazakii

2017 ◽  
Vol 9 (44) ◽  
pp. 6286-6292 ◽  
Author(s):  
Lingxian Ye ◽  
Guangying Zhao ◽  
Wenchao Dou
Keyword(s):  
Magnetic Nanoparticles ◽  
Silica Nanoparticles ◽  
Glucose Oxidase ◽  
Sandwich Immunoassay ◽  
Quantitative Detection ◽  
Cronobacter Sakazakii

A new immunoassay for the detection of C. sakazakii was developed using PGM, antibodies and glucose oxidase coated silica nanoparticles and immune magnetic nanoparticles.

scholarly journals Phthalocyanine-Functionalized Magnetic Silica Nanoparticles as Anion Chemosensors

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1632
Author(s):  
João M. M. Rodrigues ◽  
Andreia S. F. Farinha ◽  
Zhi Lin ◽  
José A. S. Cavaleiro ◽  
Augusto C. Tome ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Dimethyl Sulfoxide ◽  
Silica Nanoparticles ◽  
Hybrid Material ◽  
Limit Of Detection ◽  
Active Material ◽  
Anion Detection ◽  
Anionic Species ◽  
Magnetic Silica ◽  
Magnetic Silica Nanoparticles

Anionic species are one of the most common pollutants in residual and freshwaters. The presence of anthropogenic anions in water drastically increases the toxicity to living beings. Here, we report the preparation of a new optical active material based on tri(tosylamino)phthalocyanines grafted to ferromagnetic silica nanoparticles for anion detection and removal. The new unsymmetrical phthalocyanines (Pcs) proved to be excellent chemosensors for several anions (AcO−, Br−, Cl−, CN−, F−, H2PO4−, HSO4−, NO2−, NO3−, and OH−) in dimethyl sulfoxide (DMSO). Furthermore, the Pcs were grafted onto magnetic nanoparticles. The resulting novel hybrid material showed selectivity and sensitivity towards CN−, F−, and OH− anions in DMSO with limit of detection (LoD) of ≈4.0 µM. In water, the new hybrid chemosensor demonstrated selectivity and sensitivity for CN− and OH− anions with LoD of ≈0.2 µM. The new hybrids are easily recovered using a magnet, allowing recyclability and reusability, after acidic treatment, without losing the sensing proprieties.

An ultrasensitive nanobiohybrid platform for glucose electrochemical biosensing based on ferrocenyl iminopropyl-modified silica nanoparticles

RSC Advances ◽  
2016 ◽  
Vol 6 (52) ◽  
pp. 46238-46243 ◽  
Author(s):  
M. Saadaoui ◽  
M. Braiek ◽  
N. Jaffrezic-Renault ◽  
N. Raouafi
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Silica Nanoparticles ◽  
Glucose Oxidase ◽  
Bovine Serum ◽  
Silica Nanoparticle ◽  
Modified Silica ◽  
Electrochemical Biosensing ◽  
Modified Silica Nanoparticles

A novel nanobiohybrid platform based on ferrocenyl iminopropyl-modified silica nanoparticle conjugates (fap-SiNPs), entrapped in glucose oxidase (GOx) and bovine serum albumin cross-linked with glutaraldehyde, was developed.

Fabrication of mono-dispersed silica-coated quantum dot-assembled magnetic nanoparticles

RSC Advances ◽  
2015 ◽  
Vol 5 (41) ◽  
pp. 32072-32077 ◽  
Author(s):  
San Kyeong ◽  
Cheolhwan Jeong ◽  
Han Young Kim ◽  
Do Won Hwang ◽  
Homan Kang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Quantum Dot ◽  
Silica Nanoparticles ◽  
Fluorescent Properties

Quantum dots-embedded silica nanoparticles with an iron oxide NP core were prepared and exhibited super-paramagnetic and highly fluorescent properties.

scholarly journals Ferrocene-Modified Polyelectrolyte Film-Coated Electrode and Its Application in Glucose Detection

Polymers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 551 ◽  
Author(s):  
Zhiping Jiang ◽  
Yonggang Shangguan ◽  
Qiang Zheng
Keyword(s):  
Electron Transfer ◽  
Glucose Oxidase ◽  
Carbon Nanomaterials ◽  
Quantitative Detection ◽  
Glucose Response ◽  
Long Term Storage ◽  
Coated Electrode ◽  
Steady State Current ◽  
Polyelectrolyte Film

A polyelectrolyte film-coated electrode for the quantitative detection of glucose was reported. Carbon nanotubes, graphene oxide and polyelectrolyte with a ferrocenyl group were used to modify an enzyme electrode to facilitate the electron transfer between glucose oxidase and the electrode. Cyclic voltammetry and amperometric methods were adopted to investigate the effects of different polyelectrolytes and carbon nanomaterials on the electrochemical properties of enzyme electrodes. The results indicate that the ferrocenyl groups on a polyelectrolyte skeleton act as a mediator between the redox center of glucose oxidase and the electrode, which efficiently enhances the electron transfer between a glassy carbon electrode and glucose oxidase. The calibration curve of the sensor shows a linear range from 0.2 to 5 mM for glucose response. The sensor can achieve 95% of the steady-state current within 10 s. The electrodes also present high operational stability and long-term storage stability.

Blue silica nanoparticle-based colorimetric immunoassay for detection of Salmonella pullorum

2015 ◽  
Vol 7 (20) ◽  
pp. 8647-8654 ◽  
Author(s):  
Qian Sun ◽  
Guangying Zhao ◽  
Wenchao Dou
Keyword(s):  
Silica Nanoparticles ◽  
Silica Nanoparticle ◽  
Quantitative Detection ◽  
Salmonella Pullorum ◽  
Colorimetric Immunoassay

A colorimetric immunoassay based on blue silica nanoparticles (blue-SiNPs) was developed for quantitative detection of Salmonella pullorum (S. pullorum).

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