scholarly journals Enzymatic digestion as a tool for removing proteinaceous templates from molecularly imprinted polymers

2017 ◽  
Vol 9 (31) ◽  
pp. 4496-4503 ◽  
Author(s):  
Júlia Erdőssy ◽  
Eszter Kassa ◽  
Anita Farkas ◽  
Viola Horváth
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Enzymatic Digestion ◽  
Proteolytic Digestion ◽  
Efficient Removal ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Digestion Procedure ◽  
Pre Treatment

A proteolytic digestion procedure including pre-treatment steps is proposed for the efficient removal of protein templates from molecularly imprinted polymers.

scholarly journals Recent Advances and Future Trends in the Detection of Contaminants by Molecularly Imprinted Polymers in Food Samples

2020 ◽  
Vol 8 ◽  
Author(s):  
Mingkun Gao ◽  
Yuhang Gao ◽  
Ge Chen ◽  
Xiaodong Huang ◽  
Xiaomin Xu ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Organic Dyes ◽  
Selective Adsorption ◽  
Food Samples ◽  
Future Trends ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Brief Assessment ◽  
Technical Issues ◽  
Pre Treatment

Drug residues, organic dyes, heavy metals, and other chemical pollutants not only cause environmental pollution, but also have a serious impact on food safety. Timely and systematic summary of the latest scientific advances is of great importance for the development of new detection technologies. In particular, molecularly imprinted polymers (MIPs) can mimic antibodies, enzymes and other biological molecules to recognize, enrich, and separate contaminants, with specific recognition, selective adsorption, high affinity, and strong resistance characteristics. Therefore, MIPs have been widely used in chemical analysis, sensing, and material adsorption. In this review, we first describe the basic principles and production processes of molecularly imprinted polymers. Secondly, an overview of recent applications of molecularly imprinted polymers in sample pre-treatment, sensors, chromatographic separation, and mimetic enzymes is highlighted. Finally, a brief assessment of current technical issues and future trends in molecularly imprinted polymers is also presented.

Preparation of molecularly imprinted polymers for the recognition of proteins via the generation of peptide-fragment binding sites by semi-covalent imprinting and enzymatic digestion

The Analyst ◽  
2015 ◽  
Vol 140 (5) ◽  
pp. 1448-1452 ◽  
Author(s):  
Hironori Taguchi ◽  
Hirobumi Sunayama ◽  
Eri Takano ◽  
Yukiya Kitayama ◽  
Toshifumi Takeuchi
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Binding Sites ◽  
Enzymatic Digestion ◽  
Peptide Fragment ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

An acryloyl protein was copolymerized with a crosslinker, followed by enzymatic digestion, yielding protein imprinted polymers bearing peptide-fragment binding sites.

Application of umbelliferone molecularly imprinted polymer in analysis of plant samples

2013 ◽  
Vol 67 (5) ◽  
Author(s):  
Katarína Hroboňová ◽  
Andrea Spevak ◽  
Ľubica Spišská ◽  
Jozef Lehotay ◽  
Jozef Čižmárik
Keyword(s):  
Molecularly Imprinted Polymers ◽  
High Performance ◽  
Limit Of Detection ◽  
Binding Capacity ◽  
Specific Binding ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Limit Of Determination ◽  
Pre Treatment

AbstractThe molecularly imprinted polymers (MIPs) were synthesised and the influence of the type of porogen, the nature of sample solvent, and the binding capacity of material were tested by high-performance liquid chromatography (HPLC). Umbelliferone was used as the template for imprint formation. Methacrylic acid was used as the monomer and acetonitrile, ethanol, and chloroform as porogen. Non-imprinted polymers (NIPs) were prepared by the same procedure. The highest value of the specific binding capacity (269 μg of umbelliferone per 100 mg of polymer) was obtained for polymers prepared in chloroform as porogen and methanol/water (φ r = 1: 1) as the sample solvent. The group-selective MIP was used as sorbent for the SPE pre-treatment of umbelliferone from plant extracts prior to HPLC analysis. Analysis of the spiked samples showed good recoveries (> 77 %). The limit of detection, limit of determination, and repeatability of the method were also calculated.

Preparation of Benzonic Acid Molecularly Imprinted Polymers and Its Adsorption Property

2010 ◽  
Vol 38 (3) ◽  
pp. 401-404
Author(s):  
Da-Wei LOU ◽  
Ying-Jie YANG ◽  
Guang HUANG ◽  
Ping-Li PU ◽  
Xin-Qing LEE ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Adsorption Property ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

scholarly journals Adsorption Capacity and Selectivity of Molecularly Imprinted Polymers towards β-Sitosterol

2019 ◽  
Vol 31 (11) ◽  
pp. 2527-2531
Author(s):  
St. Fauziah ◽  
N.H. Soekamto ◽  
P. Budi ◽  
P. Taba
Keyword(s):  
Adsorption Capacity ◽  
Molecularly Imprinted Polymers ◽  
Solid Phase ◽  
Polymerization Reaction ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Sample Extract ◽  
Uv Visible ◽  
Adsorption Desorption ◽  
Isothermal Equation

Molecularly imprinted polymers (MIP) as an adsorbent has been synthesized using β-sitosterol as molecule template on free radical polymerization reaction. The capacity and selectivity of the adsorption from MIP to β-sitosterol was studied in this study. The β-sitosterol concentration in the adsorption-desorption test and the MIP selectivity test were analyzed by UV-visible and HPLC. The MIP obtained from the synthesis results in a high adsorption capacity. Based on the Freundlich adsorption isothermal equation, the adsorption capacity (k) was found to be 1.24 mg/g. The MIP can adsorb 100 % β-sitosterol while cholesterol was only 3 %. The MIP is most selective to β-sitosterol, therefore, has high potential to apply as adsorbent at solid phase extraction method to isolate β-sitosterol from sample extract.

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