Optimisation of in vitro sample preparation for LC-MS metabolomics applications on HepaRG cell cultures

2017 ◽  
Vol 9 (24) ◽  
pp. 3704-3712 ◽  
Author(s):  
Matthias Cuykx ◽  
Olivier Mortelé ◽  
Robim M. Rodrigues ◽  
Tamara Vanhaecke ◽  
Adrian Covaci
Keyword(s):  
Sample Preparation ◽  
Cell Cultures ◽  
Liquid Extraction ◽  
Heparg Cell ◽  
Liquid Liquid Extraction

The addition of stabilizers during liquid–liquid extraction improves the precision of untargeted LC-MS metabolomics workflows.

scholarly journals Application of the Dehydration Homogeneous Liquid–Liquid Extraction (DHLLE) Sample Preparation Method for Fingerprinting of Honey Volatiles

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2277
Author(s):  
Piotr M. Kuś ◽  
Igor Jerković
Keyword(s):  
Carboxylic Acid ◽  
Sample Preparation ◽  
Preparation Method ◽  
Liquid Extraction ◽  
Sample Preparation Method ◽  
Phenyllactic Acid ◽  
Homogeneous Liquid ◽  
Liquid Liquid Extraction ◽  
Wide Range ◽  
Chemical Groups

Recently, we proposed a new sample preparation method involving reduced solvent and sample usage, based on dehydration homogeneous liquid–liquid extraction (DHLLE) for the screening of volatiles and semi-volatiles from honey. In the present research, the method was applied to a wide range of honeys (21 different representative unifloral samples) to determine its suitability for detecting characteristic honey compounds from different chemical classes. GC-FID/MS disclosed 130 compounds from different structural and chemical groups. The DHLLE method allowed the extraction and identification of a wide range of previously reported specific and nonspecific marker compounds belonging to different chemical groups (including monoterpenes, norisoprenoids, benzene derivatives, or nitrogen compounds). For example, DHLLE allowed the detection of cornflower honey chemical markers: 3-oxo-retro-α-ionols, 3,4-dihydro-3-oxoedulan, phenyllactic acid; coffee honey markers: theobromine and caffeine; linden honey markers: 4-isopropenylcyclohexa-1,3-diene-1-carboxylic acid and 4-(2-hydroxy-2-propanyl)cyclohexa-1,3-diene-1-carboxylic acid, as well as furan derivatives from buckwheat honey. The obtained results were comparable with the previously reported data on markers of various honey varieties. Considering the application of much lower volumes of very common reagents, DHLLE may provide economical and ecological advantages as an alternative sample preparation method for routine purposes.

The Benefits and Limitations of Methods Development in Solid Phase Extraction: Mini Review

2014 ◽  
Vol 69 (4) ◽  
Author(s):  
Norfahana Abd-Talib ◽  
Siti Hamidah Mohd-Setapar ◽  
Aidee Kamal Khamis
Keyword(s):  
Sample Preparation ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Liquid Extraction ◽  
Phase Extraction ◽  
Methods Development ◽  
Liquid Liquid Extraction ◽  
Target Analytes ◽  
Two Phases ◽  
Preparation Techniques

Over recent years, there has been an explosive growth of sample preparation techniques. Sample preparation is in most cases meant to be the isolation online or offline concentration of some components of interest or target analytes. Solid phase extraction (SPE) is a very popular technique nowadays in sample preparation. The principal is quite similar with liquid- liquid extraction (LLE) which involves partition of solutes between two phases. But, there are some differences between them and some benefits and limitations of difference types of SPE technique like presented in this paper.

scholarly journals Comparison of two sample preparation procedures for HPLC determination of ochratoxin A

2009 ◽  
Vol 61 (4) ◽  
pp. 639-644 ◽  
Author(s):  
Gorica Vukovic ◽  
Snezana Pavlovic ◽  
M.S. Ristic
Keyword(s):  
Sample Preparation ◽  
Phosphoric Acid ◽  
Ochratoxin A ◽  
Chromatographic Determination ◽  
Liquid Extraction ◽  
Immunoaffinity Column ◽  
Maize Flour ◽  
Liquid Liquid Extraction ◽  
Immunoaffinity Columns

In preparation of samples for chromatographic determination of ochratoxin A, two types of columns were used for sample cleanup (SPE and immunoaffinity columns). The first method consisted of liquid-liquid extraction with a mixture of chloroform and phosphoric acid, followed by ion-exchange cleanup on Waters Oasis MAX columns. The sec?ond method consisted of extraction with a mixture of water and methanol, followed by LCTech OtaCLEAN immunoaf?finity column cleanup. Recoveries of the methods were determined at three levels in three repetitions for maize flour, and they were 84% (%RSD = 19.2) for the first method of sample preparation and 101% (%RSD = 2.2) for the second method. Values of LOQ for OTA were 0.25 and 1.00 ?g/kg for the IAC and SPE clean-up procedures, respectively. Both methods comply with present regulations, but the MAX sample clean-up procedure should be used as an alternative, since the immunoaffinity column clean-up procedure is characterized by better reproducibility, accuracy, and efficiency.

scholarly journals IN VITRO ANTIOXIDANT AND ANTIBACTERIAL POTENTIAL OF MEDICINAL PLANT CERASTIUM FONTANUM

2021 ◽  
Vol 51 (3) ◽  
pp. 145-151
Author(s):  
Himayat Ullah ◽  
Muhammad Mustafa ◽  
Muhammad Israr ◽  
Zahid Ali ◽  
M. Nouman ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Liquid Extraction ◽  
New Drugs ◽  
Diffusion Method ◽  
Aqueous Fraction ◽  
Gram Negative ◽  
Liquid Liquid Extraction ◽  
Antibacterial Potential ◽  
Agar Well Diffusion Method

Naturally God gifted medicinal plants which contain more potentially active compounds their characterization and isolation are very important and can provide us a great help in making new drugs to cure many diseases. Our current attempt was made to obtain the ethanolic extracts of medicinal plant Cerastium fontanum using a hot continuous soxhlet process and also via maceration method. The concentrated dried fraction extracts of Cerastium fontanum such as aqueous, n-hexane, ethyl acetate and dichloromethane were achieved using liquid-liquid extraction which were then evaluated for in vitro antioxidants potential using DPPH (1,1-diphenyl-2-picryl-hydrazyl) assay and also agar-well diffusion method were used for antimicrobial potential. In vitro antioxidants inhibition potential were measured through spectrophotometer at various concentration (500 to 62.5µg/mL) prepared in standard solvent. Among all extracts aqueous fraction showed IC50 = 2.9 ± 0.05mg/mL maximum potency towards stable DPPH much closer to standard control acarbose IC50 = 2.61 ± 0.01mg/mL. The antibacterial activity results indicated that all fractions found active against both strains (gram positive and gram negative) of bacteria. In various extracts only the aqueous fraction extracts showed excellent inhibition potential against Staphylococcus aureus and Escherichia Coli. While dichloromethane fraction extracts were found active only with gram negative strain. Thus the Cerastium fontanum extracts possess much higher inhibition potential then standard available antibiotics in the market.

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