scholarly journals A comparison of colorimetric assays detecting hydrogen peroxide in leaf extracts

2017 ◽  
Vol 9 (15) ◽  
pp. 2357-2360 ◽  
Author(s):  
Anikó Mátai ◽  
Éva Hideg
Keyword(s):  
Hydrogen Peroxide ◽  
Stress Responses ◽  
Plant Stress ◽  
Oxygen Species ◽  
Oxidizing Agent ◽  
Leaf Extracts ◽  
Signal Molecule ◽  
Central Molecule ◽  
Plant Stress Responses ◽  
Colorimetric Assays

Hydrogen peroxide (H2O2) is a central molecule in plant stress responses as a potential oxidizing agent or a signal molecule, depending on its localization and cellular concentrations. The work compares the versatility of three simple and rapid, potentially high through-put photometric assays to detect this reactive oxygen species in leaf extracts.

Reactive oxygen species as signals that modulate plant stress responses and programmed cell death

BioEssays ◽  
2006 ◽  
Vol 28 (11) ◽  
pp. 1091-1101 ◽  
Author(s):  
Tsanko S. Gechev ◽  
Frank Van Breusegem ◽  
Julie M. Stone ◽  
Iliya Denev ◽  
Christophe Laloi
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Stress Responses ◽  
Plant Stress ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Plant Stress Responses

Dual action of the active oxygen species during plant stress responses

2000 ◽  
Vol 57 (5) ◽  
pp. 779-795 ◽  
Author(s):  
J. Dat ◽  
S. Vandenabeele ◽  
E. Vranov� ◽  
M. Van Montagu ◽  
D. Inz� * ◽  
...  
Keyword(s):  
Stress Responses ◽  
Active Oxygen Species ◽  
Plant Stress ◽  
Active Oxygen ◽  
Oxygen Species ◽  
Plant Stress Responses ◽  
Dual Action

scholarly journals d-Cysteine-Induced Rapid Root Abscission in the Water Fern Azolla Pinnata: Implications for the Linkage between d-Amino Acid and Reactive Sulfur Species (RSS) in Plant Environmental Responses

Antioxidants ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 411 ◽  
Author(s):  
Hideo Yamasaki ◽  
Masahiro P. Ogura ◽  
Katsumi A. Kingjoe ◽  
Michael F. Cohen
Keyword(s):  
Stress Responses ◽  
Reactive Nitrogen Species ◽  
Plant Stress ◽  
Signaling Cascades ◽  
Oxygen Species ◽  
Sulfur Species ◽  
Chemical Inducers ◽  
Plant Stress Responses ◽  
Reactive Sulfur Species ◽  
Environmental Responses

Reactive Oxygen Species (ROS) and Reactive Nitrogen Species (RNS) have been proposed as universal signaling molecules in plant stress responses. There are a growing number of studies suggesting that hydrogen sulfide (H2S) or Reactive Sulfur Species (RSS) are also involved in plant abiotic as well as biotic stress responses. However, it is still a matter of debate as to how plants utilize those RSS in their signaling cascades. Here, we demonstrate that d-cysteine is a novel candidate for bridging our gap in understanding. In the genus of the tiny water-floating fern Azolla, a rapid root abscission occurs in response to a wide variety of environmental stimuli as well as chemical inducers. We tested five H2S chemical donors, Na2S, GYY4137, 5a, 8l, and 8o, and found that 5a showed a significant abscission activity. Root abscission also occurred with the polysulfides Na2S2, Na2S3, and Na2S4. Rapid root abscission comparable to other known chemical inducers was observed in the presence of d-cysteine, whereas l-cysteine showed no effect. We suggest that d-cysteine is a physiologically relevant substrate to induce root abscission in the water fern Azolla.

Hydrogen sulfide: a multi-tasking signal molecule in the regulation of oxidative stress responses

2020 ◽  
Vol 71 (10) ◽  
pp. 2862-2869 ◽  
Author(s):  
Tao Chen ◽  
Mimi Tian ◽  
Yi Han
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Sulfide ◽  
Stress Responses ◽  
Stomatal Closure ◽  
Plant Stress ◽  
Signal Molecule ◽  
Environmental Threats ◽  
Enhanced Production ◽  
Response Network ◽  
Plant Stress Responses

Abstract Accumulating evidence suggests that hydrogen sulfide (H2S) is an important signaling molecule in plant environmental interactions. The consensus view amongst plant scientists is that environmental stress leads to enhanced production and accumulation of reactive oxygen species (ROS). H2S interacts with the ROS-mediated oxidative stress response network at multiple levels, including the regulation of ROS-processing systems by transcriptional or post-translational modifications. H2S–ROS crosstalk also involves other interacting factors, including nitric oxide, and can affect key cellular processes like autophagy. While H2S often functions to prevent ROS accumulation, it can also act synergistically with ROS signals in processes such as stomatal closure. In this review, we summarize the mechanisms of H2S action and the multifaceted roles of this molecule in plant stress responses. Emphasis is placed on the interactions between H2S, ROS, and the redox signaling network that is crucial for plant defense against environmental threats.

scholarly journals Versatile Physiological Functions of Plant GSK3-Like Kinases

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 697
Author(s):  
Juan Mao ◽  
Wenxin Li ◽  
Jing Liu ◽  
Jianming Li
Keyword(s):  
Stress Responses ◽  
Glycogen Synthase ◽  
Plant Stress ◽  
Normal Growth ◽  
Growth Conditions ◽  
Genetic Studies ◽  
Physiological Functions ◽  
Environmental Signals ◽  
Plant Stress Responses ◽  
Diverse Plant

The plant glycogen synthase kinase 3 (GSK3)-like kinases are highly conserved protein serine/threonine kinases that are grouped into four subfamilies. Similar to their mammalian homologs, these kinases are constitutively active under normal growth conditions but become inactivated in response to diverse developmental and environmental signals. Since their initial discoveries in the early 1990s, many biochemical and genetic studies were performed to investigate their physiological functions in various plant species. These studies have demonstrated that the plant GSK3-like kinases are multifunctional kinases involved not only in a wide variety of plant growth and developmental processes but also in diverse plant stress responses. Here we summarize our current understanding of the versatile physiological functions of the plant GSK3-like kinases along with their confirmed and potential substrates.

scholarly journals OsNAC45 is Involved in ABA Response and Salt Tolerance in Rice

Rice ◽  
2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Xiang Zhang ◽  
Yan Long ◽  
Jingjing Huang ◽  
Jixing Xia
Keyword(s):  
Transcription Factors ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Crop Yields ◽  
Plant Stress ◽  
Root Cells ◽  
Nac Transcription Factors ◽  
Rice Plants ◽  
Plant Stress Responses

Abstract Background Salt stress threatens crop yields all over the world. Many NAC transcription factors have been reported to be involved in different abiotic stress responses, but it remains unclear how loss of these transcription factors alters the transcriptomes of plants. Previous reports have demonstrated that overexpression of OsNAC45 enhances salt and drought tolerance in rice, and that OsNAC45 may regulate the expression of two specific genes, OsPM1 and OsLEA3–1. Results Here, we found that ABA repressed, and NaCl promoted, the expression of OsNAC45 in roots. Immunostaining showed that OsNAC45 was localized in all root cells and was mainly expressed in the stele. Loss of OsNAC45 decreased the sensitivity of rice plants to ABA and over-expressing this gene had the opposite effect, which demonstrated that OsNAC45 played an important role during ABA signal responses. Knockout of OsNAC45 also resulted in more ROS accumulation in roots and increased sensitivity of rice to salt stress. Transcriptome sequencing assay found that thousands of genes were differently expressed in OsNAC45-knockout plants. Most of the down-regulated genes participated in plant stress responses. Quantitative real time RT-PCR suggested that seven genes may be regulated by OsNAC45 including OsCYP89G1, OsDREB1F, OsEREBP2, OsERF104, OsPM1, OsSAMDC2, and OsSIK1. Conclusions These results indicate that OsNAC45 plays vital roles in ABA signal responses and salt tolerance in rice. Further characterization of this gene may help us understand ABA signal pathway and breed rice plants that are more tolerant to salt stress.

Electrochemical Monitoring of Plant Stress Responses

2001 ◽  
Vol 13 (6) ◽  
pp. 451-456 ◽  
Author(s):  
Takeshi Kinpara ◽  
Yuji Murakami ◽  
Kenji Yokoyama ◽  
Eiichi Tamiya
Keyword(s):  
Stress Responses ◽  
Plant Stress ◽  
Plant Stress Responses ◽  
Electrochemical Monitoring
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