Development and validation of an LC-MS/MS method for simultaneous quantification of hesperidin and hesperetin in rat plasma for pharmacokinetic studies

2017 ◽  
Vol 9 (22) ◽  
pp. 3329-3337 ◽  
Author(s):  
Jr-Ting Lee ◽  
Li-Heng Pao ◽  
Chang-Da Hsieh ◽  
Pei-Wei Huang ◽  
Oliver Yoa-Pu Hu
Keyword(s):  
Wistar Rats ◽  
Rat Plasma ◽  
Pharmacokinetic Studies ◽  
Simultaneous Quantification ◽  
Development And Validation ◽  
Sensitive Method

A simple and sensitive method for the determination of hesperidin and hesperetin was necessary for a pharmacokinetic (PK) study in Wistar rats.

Development and validation of a reversed-phase HPLC method for determination of nitrendipine in rat plasma: application to pharmacokinetic studies

2007 ◽  
Vol 21 (4) ◽  
pp. 363-368 ◽  
Author(s):  
Vinay Kumar Venishetty ◽  
Chandrasekar Durairaj ◽  
Ramakrishna Sistla ◽  
Madhusudhan Rao Yamsani ◽  
Prakash V. Diwan
Keyword(s):  
Rat Plasma ◽  
Reversed Phase ◽  
Hplc Method ◽  
Pharmacokinetic Studies ◽  
Reversed Phase Hplc ◽  
Development And Validation

scholarly journals Development and Validation of an HPLC/MS/MS Method for Determining the Thiazolidinone PG15 in Rat Plasma

2010 ◽  
Vol 93 (4) ◽  
pp. 1215-1221
Author(s):  
FláVia D T UchôA ◽  
Eduardo C Palma ◽  
Natalia F Souza ◽  
Maria C A Lima ◽  
Suely L Galdino ◽  
...  
Keyword(s):  
Internal Standard ◽  
Ammonium Hydroxide ◽  
Rat Plasma ◽  
Negative Ion ◽  
Pharmacokinetic Studies ◽  
Plasma Samples ◽  
Isocratic Elution ◽  
Negative Ion Mode ◽  
Development And Validation

Abstract A rapid, sensitive, and simple HPLC/MS/MS method was developed and validated for the determination of (5Z,E)-3-[2-(4-chlorophenyl)-2-oxoethyl]-5-(1H-indol-3-ylmethylene)-thiazolidine-2, 4-dione (PG15) in rat plasma using chlortalidone as an internal standard (IS). Analyses were performed using a C18 column and isocratic elution with acetonitrilewater (90 + 10, v/v) containing 10 mM ammonium hydroxide (pH 8.0) as the mobile phase pumped at 0.3 mL/min. Detection was performed by MS with negative ion mode electrospray ionization. Rat plasma samples were prepared by deproteinizing with acetonitrile. Detected fragments were 395.1 > 171.9 for PG15 and 337.3 > 189.9 for the IS. Calibration curves were linear from 10 to 1000 ng/mL, with the determination coefficient >0.99. The intraday and interday precisions were less than 12.2 and 11.3, respectively. The applicability of the HPLC/MS/MS method for pharmacokinetic studies was tested using plasma samples obtained after oral administration of PG15 to rats, and it provided the necessary sensitivity, linearity, precision, accuracy, and specificity.

Development and validation of an LC-MS/MS method for the determination of DPHB in rat plasma and its application in pharmacokinetic studies

2018 ◽  
Vol 32 (12) ◽  
pp. e4373 ◽  
Author(s):  
Qi Wen ◽  
Hai-Long Li ◽  
Feng Chen ◽  
Jing-Wen Gong ◽  
Wei-Wei Guan ◽  
...  
Keyword(s):  
Rat Plasma ◽  
Pharmacokinetic Studies ◽  
Development And Validation

Development and Validation of an LC-MS/MS Method for Simultaneous Determination of Canagliflozin and Metformin HCl in Rat Plasma and its Application

2020 ◽  
Vol 16 (6) ◽  
pp. 752-762
Author(s):  
Vivek Nalawade ◽  
Vaibhav A. Dixit ◽  
Amisha Vora ◽  
Himashu Zade
Keyword(s):  
Internal Standard ◽  
Rat Plasma ◽  
Precipitation Method ◽  
Herbal Extracts ◽  
One Step ◽  
Precision And Accuracy ◽  
Development And Validation ◽  
The Impact ◽  
Metformin Hcl

Background: Food and herbal extracts rich in Quercetin (QRT) are often self-medicated by diabetics and can potentially alter the pharmacokinetics (PK) of Metformin HCl (MET) and Canagliflozin (CNG) leading to food or herb-drug interactions and reduced therapeutic efficacy. However, the impact of these flavonoids on the pharmacokinetic behaviour of MET and CNG is mostly unknown. Methods: A simple one-step protein precipitation method was developed for the determination of MET and CNG from rat plasma. The mobile phase chosen was MeOH 65% and 35% water containing 0.1% formic acid at a flow rate of 1mL/min. Results: The retention time of MET, internal standard (Valsartan) and CNG was 1.83, 6.2 and 8.2 min, respectively. The method was found to be linear in the range of 200 - 8000 ng/mL for CNG and 100 = 4000 ng/ml for MET. Precision and accuracy of the method were below 20% at LLOQ and below 15% for LQC, MQC, and HQC. Conclusion: The method was successfully applied for the determination of PK of MET and CNG by using 100 μL of rat plasma. QRT co-administration affects the PK parameters of MET and CNG. This alteration in PK parameters might be of significant use for clinicians and patients.

LC Method for the Determination of Rhaponticin in Rat Plasma, Faeces and Urine for Application to Pharmacokinetic Studies

2011 ◽  
Vol 73 (5-6) ◽  
pp. 599-603 ◽  
Author(s):  
Yingyong Zhao ◽  
Lili Wang ◽  
Hua Miao ◽  
Charlene C. Ho ◽  
Jing Li ◽  
...  
Keyword(s):  
Rat Plasma ◽  
Pharmacokinetic Studies
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