scholarly journals Microfluidic ELISA employing an enzyme substrate and product species with similar detection properties

The Analyst ◽  
2018 ◽  
Vol 143 (4) ◽  
pp. 989-998 ◽  
Author(s):  
Basant Giri ◽  
Yukari Liu ◽  
Fidelis N. Nchocho ◽  
Robert C. Corcoran ◽  
Debashis Dutta
Keyword(s):  
Chemical Reaction ◽  
Large Change ◽  
Elisa Method ◽  
Enzyme Substrate ◽  
Enzyme Label ◽  
Product Species

The reported ELISA method relaxes the requirement for an enzyme label to carry out a chemical reaction directly at the signaling region of the enzyme substrate in order to produce a large change in its detectability, thereby, significantly expanding the scope of this bioanalytical technique.

scholarly journals Diffusional Electrochemical Catalytic (EC’) Mechanism Featuring Chemical Reversibility of Regenerative Reaction-Theoretical Analysis in Cyclic Voltammetry

2020 ◽  
Vol 92 (4) ◽  
pp. 495-502
Author(s):  
Sofija Petkovska ◽  
Rubin Gulaboski
Keyword(s):  
Chemical Reaction ◽  
Catalytic Mechanism ◽  
Electrode Reaction ◽  
Time Frame ◽  
Cyclic Voltammograms ◽  
Kinetic And Thermodynamic Parameters ◽  
Enzyme Substrate ◽  
Ec Mechanism ◽  
Current Measuring ◽  
Product Red

We consider theoretically a specific electrochemical-catalytic mechanism associated with reversible regenerative chemical reaction, under conditions of cyclic staircase voltammetry (CSV). We suppose scenario in which two electrochemically inactive substrates “S” and “Y”, together with initial electrochemically active reactant Ox are present in voltammetric cell from the beginning of the experiment. Substrate “S” selectively reacts with initial electroactive reactant Ox and creates electroactive “product” Red (+ Y) in a reversible chemical fashion. The initial chemical equilibrium determines the amounts of Ox and Red available for electrode transformation at the beginning of the electrochemical experiment. Under conditions of applied potential, the electrode reaction Ox(aq) + ne– ⇋ Red(aq) occurs, producing flow of electric current. Under such circumstances, the chemical reaction coupled to the electrochemical step causes a regeneration of initial electroactive species during the time-frame of current-measuring segment in CSV. The features of cyclic voltammograms get significantly affected by the kinetics and thermodynamics of reversible regenerative reaction. We elaborate several aspects of this specific electrode mechanism, and we focus on the role of parameters related to chemical step to the features of calculated voltammograms. While we provide a specific set of results of this particular mechanism, we propose methods to get access to relevant kinetic and thermodynamic parameters relevant to regenerative chemical reaction. The results elaborated in this work can be valuable in evaluating kinetics of many drug-drug interactions, but they can be relevant to study interactions of many enzyme-substrate systems, as well.

scholarly journals Mathematical Methods for Modeling Chemical Reaction Networks

2016 ◽  
Author(s):  
Justin Carden ◽  
Casian Pantea ◽  
Gheorge Craciun ◽  
Raghu Machiraju ◽  
Parag Mallick
Keyword(s):  
Chemical Reaction ◽  
Chemical Reaction Networks ◽  
Chemical Processes ◽  
Reaction Networks ◽  
Mathematical Methods ◽  
Cellular Behavior ◽  
Substrate Protein ◽  
Enzyme Substrate ◽  
Mathematical Techniques ◽  
Sense And Respond

AbstractCancer’s cellular behavior is driven by alterations in the processes that cells use to sense and respond to diverse stimuli. Underlying these processes are a series of chemical processes (enzyme-substrate, protein-protein, etc.). Here we introduce a set of mathematical techniques for describing and characterizing these processes.

Directional observation on lipid of male nectary of Lindera megaphylla hemsl. by the rapid embedding method with polyethylene glycol (PEG)

1990 ◽  
Vol 48 (3) ◽  
pp. 718-719
Author(s):  
Dai Dalin ◽  
Guo Jianmin
Keyword(s):  
Electron Microscope ◽  
Polyethylene Glycol ◽  
Chemical Reaction ◽  
Traditional Method ◽  
Osmium Tetroxide ◽  
Unsaturated Lipid ◽  
Embedding Method ◽  
Long Period ◽  
New Methods

Lipid cytochemistry has not yet advanced far at the EM level. A major problem has been the loss of lipid during dehydration and embedding. Although the adoption of glutaraldehyde and osmium tetroxide accelerate the chemical reaction of lipid and osmium tetroxide can react on the double bouds of unsaturated lipid to from the osmium black, osmium tetroxide can be reduced in saturated lipid and subsequently some of unsaturated lipid are lost during dehydration. In order to reduce the loss of lipid by traditional method, some researchers adopted a few new methods, such as the change of embedding procedure and the adoption of new embedding media, to solve the problem. In a sense, these new methods are effective. They, however, usually require a long period of preparation. In this paper, we do research on the fiora nectary strucure of lauraceae by the rapid-embedding method wwith PEG under electron microscope and attempt to find a better method to solve the problem mentioned above.

Chemical Reaction in Microdroplets with Different Sizes Containing CdSe/ZnS Quantum Dot and Organic Dye

2015 ◽  
Vol E98.C (2) ◽  
pp. 123-126
Author(s):  
Takeshi FUKUDA ◽  
Tomokazu KURABAYASHI ◽  
Hikari UDAKA ◽  
Nayuta FUNAKI ◽  
Miho SUZUKI ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Chemical Reaction ◽  
Organic Dye
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