A new lateral-flow immunochromatographic strip combined with quantum dot nanobeads and gold nanoflowers for rapid detection of tetrodotoxin

The Analyst ◽  
2017 ◽  
Vol 142 (23) ◽  
pp. 4393-4398 ◽  
Author(s):  
Haicong Shen ◽  
Fei Xu ◽  
Meng Xiao ◽  
Qiangqiang Fu ◽  
Zhenzhu Cheng ◽  
...  

A sensitive, rapid detection strip based on nanoparticles for tetrodotoxin detection was developed and it meets all testing requirements.

2018 ◽  
Vol 1008 ◽  
pp. 1-7 ◽  
Author(s):  
Ruili Wu ◽  
Shuai Zhou ◽  
Ting Chen ◽  
Jinjie Li ◽  
Huaibin Shen ◽  
...  

2018 ◽  
Vol 18 (10) ◽  
pp. 7190-7196 ◽  
Author(s):  
Wen-Chien Huang ◽  
Kuo-Hui Wu ◽  
Hao-Cheng Hung ◽  
Je-Chuang Wang ◽  
Shu-Chen Chang

Food Control ◽  
2019 ◽  
Vol 106 ◽  
pp. 106714 ◽  
Author(s):  
Yaqing Wang ◽  
Zhenxing Li ◽  
Hong Lin ◽  
Pramod N. Siddanakoppalu ◽  
Jing Zhou ◽  
...  

2021 ◽  
Author(s):  
Chuanlai Xu ◽  
qianqian lu ◽  
Xin-Xin Xu ◽  
Shanshan Song ◽  
aihong wu ◽  
...  

In this study, a gold labelled immunochromatographic assay was developed to detect tigecycline (TGC) in human serum. For this purpose, an anti-TGC monoclonal antibody, 2G7, was produced and characterized, and...


2021 ◽  
pp. 104063872110275
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Linfang Cheng ◽  
Hangping Yao ◽  
...  

Avian influenza A(H5) viruses (avian IAVs) pose a major threat to the economy and public health. We developed an antigen-ELISA (ag-ELISA) and a colloidal gold–based immunochromatographic strip for the rapid detection of avian A(H5) viruses. Both detection methods displayed no cross-reactivity with other viruses (e.g., other avian IAVs, infectious bursal disease virus, Newcastle disease virus, infectious bronchitis virus, avian paramyxovirus). The ag-ELISA was sensitive down to 0.5 hemagglutinin (HA) units/100 µL of avian A(H5) viruses and 7.5 ng/mL of purified H5 HA proteins. The immunochromatographic strip was sensitive down to 1 HA unit/100 µL of avian A(H5) viruses. Both detection methods exhibited good reproducibility with CVs < 10%. For 200 random poultry samples, the sensitivity and specificity of the ag-ELISA were 92.6% and 98.8%, respectively, and for test strips were 88.9% and 98.3%, respectively. Both detection methods displayed high specificity, sensitivity, and stability, making them suitable for rapid detection and field investigation of avian A(H5) viruses.


Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.


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