Detection of a few DNA copies by real-time electrochemical polymerase chain reaction

The Analyst ◽  
2017 ◽  
Vol 142 (18) ◽  
pp. 3432-3440 ◽  
Author(s):  
M. Moreau ◽  
S. Delile ◽  
A. Sharma ◽  
C. Fave ◽  
A. Perrier ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Current Work ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
First Time ◽  
Accurate Quantification

In the current work, accurate quantification over 10 to 108 DNA copies has been successfully achieved for the first time by real-time electrochemical PCR.

Epstein-Barr virus load in the peripheral blood of patients with rheumatoid arthritis: Accurate quantification using real-time polymerase chain reaction

2003 ◽  
Vol 48 (5) ◽  
pp. 1223-1228 ◽  
Author(s):  
Nathalie Balandraud ◽  
Jean Baptiste Meynard ◽  
Isabelle Auger ◽  
Helene Sovran ◽  
Benedicte Mugnier ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Peripheral Blood ◽  
Epstein Barr Virus ◽  
Chain Reaction ◽  
Barr Virus ◽  
Polymerase Chain ◽  
Epstein Barr ◽  
Accurate Quantification

scholarly journals Molecular characterization of field isolates of Avian Infectious Laryngeotracheitis Virus from different farms in Iraq

2017 ◽  
Vol 40 (2) ◽  
pp. 31-35
Author(s):  
Aida Bara Allawe
Keyword(s):  
Polymerase Chain Reaction ◽  
Positive Result ◽  
Real Time ◽  
Virus Detection ◽  
Chain Reaction ◽  
Glycoprotein G ◽  
Polymerase Chain ◽  
Labeled Probe ◽  
First Time

     This study was conducted to detect virulent isolates of avian infectious laryngeotracheitis virus in Iraq by Real Time-Polymerase Chain Reaction with the amplification of glycoprotein G gene which is responsible for virulence of the virus. Seventy samples (larynx and trachea) were collected from different farms in Iraq to investigate presence of avian infectious laryngeotracheitis virus (detection of virulent isolates from other vaccine strains). Five samples out of seventy samples were virulent isolates (positive result) by using Real Time-Polymerase Chain Reaction utilizing flurescein amidite labeled probe specific for detection of isolates that have G gene (by amplification of G gene) for the first time in Iraq. These virulent isolates were negative by using Real Time-Polymerase Chain Reaction utilizing Quasar-labeled probe specific for the detection of attenuated isolates that lack G gene and targeted a region within glycoprotein J downstream from the sequence of glycoprotein G.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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