scholarly journals On-chip conductometric detection of short DNA sequences via electro-hydrodynamic aggregation

The Analyst ◽  
2018 ◽  
Vol 143 (1) ◽  
pp. 190-199 ◽  
Author(s):  
B. Venzac ◽  
M. L. Diakité ◽  
D. Herthnek ◽  
I. Cissé ◽  
U. Bockelmann ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Microfluidic System ◽  
Label Free ◽  
Bacterial Dna ◽  
Conductometric Detection ◽  
On Chip

Label-free, conductometric detection of isothermally amplified bacterial DNA was performed in a portable microfluidic system thanks to electro-hydrodynamic aggregation.

scholarly journals Rapid real-time recirculating PCR using localized surface plasmon resonance (LSPR) and piezo-electric pumping

Lab on a Chip ◽  
2017 ◽  
Vol 17 (16) ◽  
pp. 2821-2830 ◽  
Author(s):  
J. M. Haber ◽  
P. R. C. Gascoyne ◽  
K. Sokolov
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Localized Surface Plasmon Resonance ◽  
Pathogen Detection ◽  
Microfluidic System ◽  
Localized Surface Plasmon ◽  
Label Free ◽  
Multiplex Detection ◽  
Highly Sensitive ◽  
On Chip

The paper describes a novel on-chip microfluidic system for rapid highly sensitive and label-free multiplex detection of DNA molecules with specific focus on pathogen detection.

scholarly journals Design and Manufacturing of a Disposable, Cyclo-Olefin Copolymer, Microfluidic Device for a Biosensor †

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1178 ◽  
Author(s):  
Jorge Prada ◽  
Christina Cordes ◽  
Carsten Harms ◽  
Walter Lang
Keyword(s):  
Microfluidic Device ◽  
Heating Temperature ◽  
Low Cost ◽  
Reaction Chamber ◽  
Microfluidic System ◽  
Heating Process ◽  
Design And Manufacturing ◽  
On Chip ◽  
Working Parameters ◽  
Auto Fluorescence

This contribution outlines the design and manufacturing of a microfluidic device implemented as a biosensor for retrieval and detection of bacteria RNA. The device is fully made of Cyclo-Olefin Copolymer (COC), which features low auto-fluorescence, biocompatibility and manufacturability by hot-embossing. The RNA retrieval was carried on after bacteria heat-lysis by an on-chip micro-heater, whose function was characterized at different working parameters. Carbon resistive temperature sensors were tested, characterized and printed on the biochip sealing film to monitor the heating process. Off-chip and on-chip processed RNA were hybridized with capture probes on the reaction chamber surface and identification was achieved by detection of fluorescence tags. The application of the mentioned techniques and materials proved to allow the development of low-cost, disposable albeit multi-functional microfluidic system, performing heating, temperature sensing and chemical reaction processes in the same device. By proving its effectiveness, this device contributes a reference to show the integration potential of fully thermoplastic devices in biosensor systems.

Closed-loop feedback control of microfluidic cell manipulation via deep-learning integrated sensor networks

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Ningquan Wang ◽  
Ruxiu Liu ◽  
Norh Asmare ◽  
Chia-Heng Chu ◽  
Ozgun Civelekoglu ◽  
...  
Keyword(s):  
Deep Learning ◽  
Sensor Networks ◽  
Feedback Control ◽  
Closed Loop ◽  
Microfluidic System ◽  
Cell Manipulation ◽  
Integrated Sensor ◽  
Loop Feedback ◽  
Closed Loop Feedback ◽  
On Chip

An adaptive microfluidic system changing its operational state in real-time based on cell measurements through an on-chip electrical sensor network.

scholarly journals Silicon-Quartz Microcapillary Opto-Fluidic Platform Obtained by CMOS-Compatible Die to Wafer 200 mm Dual Bonding Process

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 1018
Author(s):  
Giuseppe Fiorentino ◽  
Ben Jones ◽  
Sophie Roth ◽  
Edith Grac ◽  
Murali Jayapala ◽  
...  
Keyword(s):  
Adhesive Bonding ◽  
System Analysis ◽  
Quartz Substrate ◽  
Microfluidic System ◽  
Bonding Process ◽  
Microfluidic Channels ◽  
Fully Integrated ◽  
Lab On Chip ◽  
Human Blood Cells ◽  
On Chip

A composite, capillary-driven microfluidic system suitable for transmitted light microscopy of cells (e.g., red and white human blood cells) is fabricated and demonstrated. The microfluidic system consists of a microchannels network fabricated in a photo-patternable adhesive polymer on a quartz substrate, which, by means of adhesive bonding, is then connected to a silicon microfluidic die (for processing of the biological sample) and quartz die (to form the imaging chamber). The entire bonding process makes use of a very low temperature budget (200 °C). In this demonstrator, the silicon die consists of microfluidic channels with transition structures to allow conveyance of fluid utilizing capillary forces from the polymer channels to the silicon channels and back to the polymer channels. Compared to existing devices, this fully integrated platform combines on the same substrate silicon microfluidic capabilities with optical system analysis, representing a portable and versatile lab-on-chip device.

scholarly journals Acute On-Chip HIV Detection Through Label-Free Electrical Sensing of Viral Nano-Lysate

Small ◽  
2013 ◽  
Vol 9 (15) ◽  
pp. 2553-2563 ◽  
Author(s):  
Hadi Shafiee ◽  
Muntasir Jahangir ◽  
Fatih Inci ◽  
ShuQi Wang ◽  
Remington B. M. Willenbrecht ◽  
...  
Keyword(s):  
Label Free ◽  
Electrical Sensing ◽  
On Chip

INTEGRATED 3D MULTI-PHYSICAL SIMULATION OF A MICROFLUIDIC SYSTEM USING FINITE ELEMENT ANALYSIS

2015 ◽  
Vol 15 (06) ◽  
pp. 1540043 ◽  
Author(s):  
HAO SUN ◽  
ZHANDONG LI ◽  
JIANGUO TAO
Keyword(s):  
Finite Element ◽  
Physical Simulation ◽  
Microfluidic System ◽  
Von Mises Stress ◽  
Biomedical Science ◽  
Heating Zone ◽  
Element Analysis ◽  
Theoretical Understanding ◽  
Velocity Magnitude ◽  
On Chip

Microfluidics technology has emerged as an attractive approach in physics, chemistry and biomedical science by providing increased analytical accuracy, sensitivity and efficiency in minimized systems. Numerical simulation can improve theoretical understanding, reduce prototyping consumption, and speed up development. In this paper, we setup a 3D model of an integrated microfluidic system and study the multi-physical dynamics of the system via the finite element method (FEM). The fluid–structure interaction (FSI) of fluid and an immobilized single cell within the cell trapping component, and the on-chip thermodynamics have been analyzed. The velocity magnitude and streamline of flow field, the distribution of von Mises stress and Tresca stress on the FSI interface have been studied. In addition, the on-chip heat transfer performance and temperature distribution in the heating zone have been evaluated and analyzed respectively. The presented approach is capable of optimizing microfluidic design, and revealing the complicated mechanism of multi-physical fields. Therefore, it holds the potential for improving microfluidics application in fundamental research and clinical settings.

Establishment of screening system toward discovery of kinase inhibitors using label-free on-chip phosphorylation assays

Biosystems ◽  
2009 ◽  
Vol 97 (3) ◽  
pp. 179-185 ◽  
Author(s):  
Kazuki Inamori ◽  
Motoki Kyo ◽  
Kazuki Matsukawa ◽  
Yusuke Inoue ◽  
Tatsuhiko Sonoda ◽  
...  
Keyword(s):  
Kinase Inhibitors ◽  
Screening System ◽  
Label Free ◽  
On Chip
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