Label-free, high content screening using Raman microspectroscopy: the toxicological response of different cell lines to amine-modified polystyrene nanoparticles (PS-NH2)

The Analyst ◽  
2017 ◽  
Vol 142 (18) ◽  
pp. 3500-3513 ◽  
Author(s):  
Esen Efeoglu ◽  
Marcus A. Maher ◽  
Alan Casey ◽  
Hugh J. Byrne
Keyword(s):  
Multivariate Analysis ◽  
Cell Lines ◽  
Raman Microspectroscopy ◽  
High Content Screening ◽  
Label Free ◽  
Polystyrene Nanoparticles ◽  
Screening Technique ◽  
Cancerous Cell ◽  
Modified Polystyrene

Raman microspectroscopy as a ‘high content nanotoxicological screening technique’ with the aid of multivariate analysis, on non-cancerous and cancerous cell lines.

scholarly journals Determination of spectral markers of cytotoxicity and genotoxicity using in vitro Raman microspectroscopy: cellular responses to polyamidoamine dendrimer exposure

The Analyst ◽  
2017 ◽  
Vol 142 (20) ◽  
pp. 3848-3856 ◽  
Author(s):  
Esen Efeoglu ◽  
Alan Casey ◽  
Hugh J. Byrne
Keyword(s):  
Cellular Responses ◽  
Raman Microspectroscopy ◽  
High Content Screening ◽  
Label Free ◽  
Polyamidoamine Dendrimer ◽  
Screening Technique

Raman microspectroscopy as anin vitrolabel-free, high content screening technique to determine spectral markers of cytogenotoxicity.

scholarly journals Application of metasurface-enhanced infra-red spectroscopy to distinguish between normal and cancerous cell types

The Analyst ◽  
2019 ◽  
Vol 144 (4) ◽  
pp. 1115-1127 ◽  
Author(s):  
G. Kelp ◽  
N. Arju ◽  
A. Lee ◽  
E. Esquivel ◽  
R. Delgado ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cell Types ◽  
Label Free ◽  
Cancerous Cell ◽  
Infrared Reflection ◽  
Infra Red ◽  
Colon Cell

Metasurface-enhanced infrared reflection spectroscopic cytopathology (MEIRSC) is used for label-free distinguishing between normal and cancerous colon cell lines.

In Vitro Evaluation of Chitosan Induced Cytotoxicity against Cancerous Cell lines: MCF-7, Saos-2 and HeLa

2019 ◽  
Vol 19 ◽  
Author(s):  
Zeinab Abedian ◽  
Niloofar Jenabian ◽  
Ali Akbar Moghadamnia ◽  
Ebrahim Zabihi ◽  
Roghayeh Pourbagher ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Fibroblast Cells ◽  
Apoptosis Induction ◽  
Cytotoxic Effects ◽  
Cancerous Cell ◽  
Significant Concentration ◽  
Mcf 7

Objective/ Background: Cancer is still the most common cause of morbidity in world and new powerful anticancer agents without severe side effects from natural sources is important. Methods: The evaluation of cytotoxicity and apoptosis induction was carried out in MCF-7,HeLa and Saos-2 as cancerous cell lines with different histological origin and human fibroblast served as control normal cell. The cells were treated with different concentrations of chitosan and the cytotoxicity was determined using MTT assay after 24, 48 and 72 h .The mode of death was evaluated by flow cytometry . Results: While both types of chitosan showed significant concentration-dependently cytotoxic effects against the three cancerous cell lines, fibroblast cells showed somehow more compatibility with chitosan. On the other hand, there were no significant differences between LMWC and HMWC cytotoxicity in all cell lines. The flow cytometry results showed the apoptosis pattern of death more in Saos-2 and HeLa while necrosis was more observable with MCF7. Also higher viability with both types of chitosan was seen in fibroblast as normal cells Conclusion: Chitosan shows anticancerous effect against 3 cancerous cell lines, while it is compatible with normal diploid fibroblast cells. Furthermore, it seems that the molecular weight of chitosan does not affect its anticancerous property.

scholarly journals Differential Effects of Gold Nanoparticles and Ionizing Radiation on Cell Motility between Primary Human Colonic and Melanocytic Cells and Their Cancerous Counterparts

2021 ◽  
Vol 22 (3) ◽  
pp. 1418
Author(s):  
Elham Shahhoseini ◽  
Masao Nakayama ◽  
Terrence J. Piva ◽  
Moshi Geso
Keyword(s):  
Gold Nanoparticles ◽  
Ionizing Radiation ◽  
Cancer Cells ◽  
Cell Lines ◽  
Colorectal Adenocarcinoma ◽  
X Rays ◽  
Cell Adherence ◽  
Cancerous Cell ◽  
Primary Colon ◽  
Radiation Induced

This study examined the effects of gold nanoparticles (AuNPs) and/or ionizing radiation (IR) on the viability and motility of human primary colon epithelial (CCD841) and colorectal adenocarcinoma (SW48) cells as well as human primary epidermal melanocytes (HEM) and melanoma (MM418-C1) cells. AuNPs up to 4 mM had no effect on the viability of these cell lines. The viability of the cancer cells was ~60% following exposure to 5 Gy. Exposure to 5 Gy X-rays or 1 mM AuNPs showed the migration of the cancer cells ~85% that of untreated controls, while co-treatment with AuNPs and IR decreased migration to ~60%. In the non-cancerous cell lines gap closure was enhanced by ~15% following 1 mM AuNPs or 5 Gy treatment, while for co-treatment it was ~22% greater than that for the untreated controls. AuNPs had no effect on cell re-adhesion, while IR enhanced only the re-adhesion of the cancer cell lines but not their non-cancerous counterparts. The addition of AuNPs did not enhance cell adherence. This different reaction to AuNPs and IR in the cancer and normal cells can be attributed to radiation-induced adhesiveness and metabolic differences between tumour cells and their non-cancerous counterparts.

scholarly journals In Vitro Spectroscopy-Based Profiling of Urothelial Carcinoma: A Fourier Transform Infrared and Raman Imaging Study

Cancers ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 123
Author(s):  
Monika Kujdowicz ◽  
Wojciech Placha ◽  
Brygida Mech ◽  
Karolina Chrabaszcz ◽  
Krzysztof Okoń ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Fourier Transform ◽  
Cancer Cells ◽  
Cell Line ◽  
Cell Lines ◽  
Malignant Neoplasm ◽  
Fourier Transform Infrared ◽  
Diagnostic Tools ◽  
Label Free ◽  
Bladder Cancer Cells

Markers of bladder cancer cells remain elusive, which is a major cause of the low recognition of this malignant neoplasm and its recurrence. This implies an urgent need for additional diagnostic tools which are based on the identification of the chemism of bladder cancer. In this study, we employed label-free techniques of molecular imaging—Fourier Transform Infrared and Raman spectroscopic imaging—to investigate bladder cancer cell lines of various invasiveness (T24a, T24p, HT-1376, and J82). The urothelial HCV-29 cell line was the healthy control. Specific biomolecules discriminated spatial distribution of the nucleus and cytoplasm and indicated the presence of lipid bodies and graininess in some cell lines. The most prominent discriminators are the total content of lipids and sugar moieties as well as the presence of glycogen and other carbohydrates, un/saturated lipids, cytochromes, and a level of S-S bridges in proteins. The combination of the obtained hyperspectral database and chemometric methods showed a clear differentiation of each cell line at the level of the nuclei and cytoplasm and pointed out spectral signals which differentiated bladder cancer cells. Registered spectral markers correlated with biochemical composition changes can be associated with pathogenesis and potentially used for the diagnosis of bladder cancer and response to experimental therapies.

Label-Free Determination of the Cell Cycle Phase in Human Embryonic Stem Cells by Raman Microspectroscopy

2013 ◽  
Vol 85 (19) ◽  
pp. 8996-9002 ◽  
Author(s):  
Stanislav O. Konorov ◽  
H. Georg Schulze ◽  
James M. Piret ◽  
Michael W. Blades ◽  
Robin F. B. Turner
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Raman Microspectroscopy ◽  
Cell Cycle Phase ◽  
Cycle Phase ◽  
Label Free

Viability of cancerous cell lines as a function of energy delivered

2010 ◽  
Author(s):  
K Sankaranarayanan ◽  
R P Ramachandran ◽  
M Sriram Kumar ◽  
V Madan Kumar ◽  
S Vignesh ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancerous Cell

scholarly journals Label-Free Discrimination of Rhizobial Bacteroids and Mutants by Single-Cell Raman Microspectroscopy

2017 ◽  
Vol 89 (12) ◽  
pp. 6336-6340 ◽  
Author(s):  
Jiabao Xu ◽  
Isabel Webb ◽  
Philip Poole ◽  
Wei E. Huang
Keyword(s):  
Single Cell ◽  
Raman Microspectroscopy ◽  
Label Free

Discrimination of cancerous and non-cancerous cell lines by headspace-analysis with PTR-MS

2010 ◽  
Vol 397 (6) ◽  
pp. 2315-2324 ◽  
Author(s):  
C. Brunner ◽  
W. Szymczak ◽  
V. Höllriegl ◽  
S. Mörtl ◽  
H. Oelmez ◽  
...  
Keyword(s):  
Cell Lines ◽  
Headspace Analysis ◽  
Cancerous Cell
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