Using Raman spectroscopy to assess hemoglobin oxygenation in red blood cell concentrate: an objective proxy for morphological index to gauge the quality of stored blood?

The Analyst ◽  
2017 ◽  
Vol 142 (12) ◽  
pp. 2199-2210 ◽  
Author(s):  
Chad G. Atkins ◽  
H. Georg Schulze ◽  
Deborah Chen ◽  
Dana V. Devine ◽  
Michael W. Blades ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Morphological Index ◽  
Hemoglobin Oxygenation ◽  
Stored Blood

A relationship has been found between hemoglobin oxygenation of stored red blood cells (measured using Raman spectroscopy) and a morphological index.

scholarly journals Normal saline-induced deoxygenation of red blood cells probed by optical tweezers combined with the micro-Raman technique

RSC Advances ◽  
2019 ◽  
Vol 9 (14) ◽  
pp. 7878-7884 ◽  
Author(s):  
Jijo Lukose ◽  
Mithun N ◽  
Ganesh Mohan ◽  
Shamee Shastry ◽  
Santhosh Chidangil
Keyword(s):  
Raman Spectroscopy ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Optical Tweezers ◽  
Red Blood Cell ◽  
Normal Saline ◽  
Blood Cells ◽  
Micro Raman Spectroscopy ◽  
Optically Trapped

Micro-Raman spectroscopy of optically trapped live red blood cell demonstrates normal saline induced deoxygenation.

Raman spectroscopy of stored red blood cell concentrate within sealed transfusion blood bags

The Analyst ◽  
2018 ◽  
Vol 143 (24) ◽  
pp. 6006-6013 ◽  
Author(s):  
M. Z. Vardaki ◽  
C. G. Atkins ◽  
H. G. Schulze ◽  
D. V. Devine ◽  
K. Serrano ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Blood Transfusion ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Spectral Information ◽  
Stored Blood

Spectral information relevant to the quality of stored blood can be obtained in situ through sealed blood transfusion bags using a commercially available instrument.

Raman spectroscopy as a novel tool for monitoring biochemical changes and inter-donor variability in stored red blood cell units

The Analyst ◽  
2016 ◽  
Vol 141 (11) ◽  
pp. 3319-3327 ◽  
Author(s):  
Chad G. Atkins ◽  
Kevin Buckley ◽  
Deborah Chen ◽  
H. Georg Schulze ◽  
Dana V. Devine ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Biochemical Changes ◽  
Donor Variability

Raman spectroscopy has been used to retrieve biochemical information from the supernatant of stored red blood cells (RBCs), demonstrating that some units of donated RBCs accumulate lactate much more readily than others.

Non-invasive spectroscopy of transfusable red blood cells stored inside sealed plastic blood-bags

The Analyst ◽  
2016 ◽  
Vol 141 (5) ◽  
pp. 1678-1685 ◽  
Author(s):  
K. Buckley ◽  
C. G. Atkins ◽  
D. Chen ◽  
H. G. Schulze ◽  
D. V. Devine ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Non Invasive

Spatially Offset Raman spectroscopy (SORS) has been used to retrieve biochemical information from red blood cell concentrate within sealed PVC bags, demonstrating the potential of SORS to non-invasively assess the condition of the cells prior to transfusion.

Microfluidic electrical impedance assessment of red blood cell mediated microvascular occlusion

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yuncheng Man ◽  
Debnath Maji ◽  
Ran An ◽  
Sanjay Ahuja ◽  
Jane A Little ◽  
...  
Keyword(s):  
Sickle Cell Disease ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Electrical Impedance ◽  
Cell Disease ◽  
Blood Disorders

Alterations in the deformability of red blood cells (RBCs), occurring in hemolytic blood disorders such as sickle cell disease (SCD), contributes to vaso-occlusion and disease pathophysiology. However, there are few...

Permeation of the luminal capillary glycocalyx is determined by hyaluronan

1999 ◽  
Vol 277 (2) ◽  
pp. H508-H514 ◽  
Author(s):  
Charmaine B. S. Henry ◽  
Brian R. Duling
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Enzyme Treatment ◽  
Apical Surface ◽  
Bright Field ◽  
Endothelial Surface ◽  
The Difference

The endothelial cell glycocalyx influences blood flow and presents a selective barrier to movement of macromolecules from plasma to the endothelial surface. In the hamster cremaster microcirculation, FITC-labeled Dextran 70 and larger molecules are excluded from a region extending almost 0.5 μm from the endothelial surface into the lumen. Red blood cells under normal flow conditions are excluded from a region extending even farther into the lumen. Examination of cultured endothelial cells has shown that the glycocalyx contains hyaluronan, a glycosaminoglycan which is known to create matrices with molecular sieving properties. To test the hypothesis that hyaluronan might be involved in establishing the permeation properties of the apical surface glycocalyx in vivo, hamster microvessels in the cremaster muscle were visualized using video microscopy. After infusion of one of several FITC-dextrans (70, 145, 580, and 2,000 kDa) via a femoral cannula, microvessels were observed with bright-field and fluorescence microscopy to obtain estimates of the anatomic diameters and the widths of fluorescent dextran columns and of red blood cell columns (means ± SE). The widths of the red blood cell and dextran exclusion zones were calculated as one-half the difference between the bright-field anatomic diameter and the width of the red blood cell column or dextran column. After 1 h of treatment with active Streptomyces hyaluronidase, there was a significant increase in access of 70- and 145-kDa FITC-dextrans to the space bounded by the apical glycocalyx, but no increase in access of the red blood cells or in the anatomic diameter in capillaries, arterioles, and venules. Hyaluronidase had no effect on access of FITC-Dextrans 580 and 2,000. Infusion of a mixture of hyaluronan and chondroitin sulfate after enzyme treatment reconstituted the glycocalyx, although treatment with either molecule separately had no effect. These results suggest that cell surface hyaluronan plays a role in regulating or establishing permeation of the apical glycocalyx to macromolecules. This finding and our prior observations suggest that hyaluronan and other glycoconjugates are required for assembly of the matrix on the endothelial surface. We hypothesize that hyaluronidase creates a more open matrix, enabling smaller dextran molecules to penetrate deeper into the glycocalyx.

Susceptibility of density-fractionated erythrocytes to subhaemolytic mechanical shear stress

2018 ◽  
Vol 42 (3) ◽  
pp. 151-157 ◽  
Author(s):  
Antony P McNamee ◽  
Kieran Richardson ◽  
Jarod Horobin ◽  
Lennart Kuck ◽  
Michael J Simmonds
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Shear Stresses ◽  
Mechanical Stimuli ◽  
Cell Deformability ◽  
Red Blood Cell Deformability ◽  
Cell Age ◽  
Low Shear

Introduction: Accumulating evidence demonstrates that subhaemolytic mechanical stresses, typical of circulatory support, induce physical and biochemical changes to red blood cells. It remains unclear, however, whether cell age affects susceptibility to these mechanical forces. This study thus examined the sensitivity of density-fractionated red blood cells to sublethal mechanical stresses. Methods: Red blood cells were isolated and washed twice, with the least and most dense fractions being obtained following centrifugation (1500 g × 5 min). Red blood cell deformability was determined across an osmotic gradient and a range of shear stresses (0.3–50 Pa). Cell deformability was also quantified before and after 300 s exposure to shear stresses known to decrease (64 Pa) or increase (10 Pa) red blood cell deformability. The time course of accumulated sublethal damage that occurred during exposure to 64 Pa was also examined. Results: Dense red blood cells exhibited decreased capacity to deform when compared with less dense cells. Cellular response to mechanical stimuli was similar in trend for all red blood cells, independent of density; however, the magnitude of impairment in cell deformability was exacerbated in dense cells. Moreover, the rate of impairment in cellular deformability, induced by 64 Pa, was more rapid for dense cells. Relative improvement in red blood cell deformability, due to low-shear conditioning (10 Pa), was consistent for both cell populations. Conclusion: Red blood cell populations respond differently to mechanical stimuli: older (more dense) cells are highly susceptible to sublethal mechanical trauma, while cell age (density) does not appear to alter the magnitude of improved cell deformability following low-shear conditioning.

scholarly journals Small Red Blood Cell Fraction on the UF-1000i Urine Analyzer as a Screening Tool to Detect Dysmorphic Red Blood Cells for Diagnosing Glomerulonephritis

2019 ◽  
Vol 39 (3) ◽  
pp. 271
Author(s):  
Hyunjung Kim ◽  
Young Ok Kim ◽  
Yonggoo Kim ◽  
Jin-Soon Suh ◽  
Eun-Jung Cho ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Screening Tool ◽  
Cell Fraction
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