Gold nanodisc arrays as near infrared metal-enhanced fluorescence platforms with tuneable enhancement factors

2017 ◽  
Vol 5 (4) ◽  
pp. 917-925 ◽  
Author(s):  
J. Pang ◽  
I. G. Theodorou ◽  
A. Centeno ◽  
P. K. Petrov ◽  
N. M. Alford ◽  
...  
Keyword(s):  
Near Infrared ◽  
Fluorescence Enhancement ◽  
Structural Characteristics ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Nir Fluorescence ◽  
Enhancement Factors

Au nanodisc arrays with nanoscale control of their structural characteristics, allow significant NIR fluorescence enhancement with tunable sensitivities.

Fluorescence enhancement from single gold nanostars: towards ultra-bright emission in the first and second near-infrared biological windows

Nanoscale ◽  
2018 ◽  
Vol 10 (33) ◽  
pp. 15854-15864 ◽  
Author(s):  
Ioannis G. Theodorou ◽  
Qianfan Jiang ◽  
Lukas Malms ◽  
Xiangyu Xie ◽  
R. Charles Coombes ◽  
...  
Keyword(s):  
Single Particle ◽  
Colloidal Gold ◽  
Near Infrared ◽  
Fluorescence Enhancement ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Biologically Relevant ◽  
Gold Nanostars ◽  
Bright Emission ◽  
Biological Windows

Single-particle metal enhanced fluorescence from colloidal gold nanostars (AuNSs) enables super bright fluorescence from biologically relevant near-infrared fluorophores.

Lighting up Plasmonic Nanostar Colloids for Metal-Enhanced Fluorescence under Two-Photon Near-Infrared Excitation

2018 ◽  
Vol 122 (34) ◽  
pp. 19823-19830 ◽  
Author(s):  
Lixia Zhou ◽  
Kuan-Jen Chen ◽  
Jacob D. Ramsey ◽  
C. Kyle Almlie ◽  
Sean M. Burrows
Keyword(s):  
Near Infrared ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Two Photon ◽  
Infrared Excitation

scholarly journals Metal enhanced fluorescence biosensing: from ultra-violet towards second near-infrared window

Nanoscale ◽  
2018 ◽  
Vol 10 (45) ◽  
pp. 20914-20929 ◽  
Author(s):  
Sarah Madeline Fothergill ◽  
Caoimhe Joyce ◽  
Fang Xie
Keyword(s):  
Near Infrared ◽  
Survival Rates ◽  
Ultra Violet ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Infrared Window

To increase disease survival rates, there is a vital need for diagnosis at very preliminary stages.

scholarly journals Understanding metal-enhanced fluorescence and structural properties in Au@Ag core–shell nanocubes

RSC Advances ◽  
2019 ◽  
Vol 9 (50) ◽  
pp. 29232-29237 ◽  
Author(s):  
Dae-Woong Jung ◽  
Jun Min Kim ◽  
Hyung Joong Yun ◽  
Gi-Ra Yi ◽  
Jung Young Cho ◽  
...  
Keyword(s):  
Structural Properties ◽  
Building Block ◽  
Fluorescence Enhancement ◽  
Core Shell ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Optical Functions ◽  
Fluorescence Quencher

The unique ring-like building block of Au@AgNCs has dual optical functions as a fluorescence quencher and fluorescence enhancement medium.

Influence of silver grain size, roughness, and profile on the extraordinary fluorescence enhancement capabilities of grating coupled surface plasmon resonance

RSC Advances ◽  
2015 ◽  
Vol 5 (96) ◽  
pp. 78534-78544 ◽  
Author(s):  
A. J. Wood ◽  
B. Chen ◽  
S. Pathan ◽  
S. Bok ◽  
C. J. Mathai ◽  
...  
Keyword(s):  
Grain Size ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Metal Film ◽  
Fluorescence Enhancement ◽  
Enhanced Fluorescence ◽  
Film Properties ◽  
Metal Enhanced Fluorescence

Silver gratings with different metal film properties and structures were examined to determine their effect on metal enhanced fluorescence.

scholarly journals Correction: Metal enhanced fluorescence biosensing: from ultra-violet towards second near-infrared window

Nanoscale ◽  
2018 ◽  
Vol 10 (45) ◽  
pp. 21533-21533
Author(s):  
Sarah Madeline Fothergill ◽  
Caoimhe Joyce ◽  
Fang Xie
Keyword(s):  
Near Infrared ◽  
Ultra Violet ◽  
Enhanced Fluorescence ◽  
Metal Enhanced Fluorescence ◽  
Infrared Window

Correction for ‘Metal enhanced fluorescence biosensing: from ultra-violet towards second near-infrared window’ by Sarah Madeline Fothergill et al., Nanoscale, 2018, DOI: 10.1039/c8nr06156d.

scholarly journals Ligand-Free BaF2: Nd Nanoparticles With Low Cytotoxicity, High Stability and Enhanced Fluorescence Intensity as NIR-II Imaging Probes

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiaoxia Cui ◽  
Yantao Xu ◽  
Shengfei She ◽  
Xusheng Xiao ◽  
Chaoqi Hou ◽  
...  
Keyword(s):  
Near Infrared ◽  
Superior Performance ◽  
Excitation Wavelength ◽  
Enhanced Fluorescence ◽  
High Penetration ◽  
Nir Fluorescence ◽  
Ligand Free ◽  
Imaging Probes ◽  
Low Cytotoxicity

Ligand-free BaF2:Nd nanoparticles (NPs) with a size of 10 nm were fabricated by a novel synthetic route in the liquid phase. A transparent dispersion of the BaF2:Nd NPs mixed with propanetriol and DMSO-d6 was done. Highly stable and outstanding near-infrared (NIR) fluorescence centered at 1,058 nm was detected using an excitation wavelength of 808 nm laser. Moreover, the dispersion can be found to be stable for over 1 month, and the cytotoxicity of the BaF2:Nd NP dispersion has also been studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The superior performance of these NPs exhibits their great potential application in high-contrast and high-penetration in vivo imaging.

scholarly journals Multifunctional Thio-Stabilized Gold Nanoparticles for Near-Infrared Fluorescence Detection and Imaging of Activated Caspase-3

2021 ◽  
Vol 17 ◽  
Author(s):  
Jinda Fan ◽  
Philip P. Cheney ◽  
Sharon Bloch ◽  
Baogang Xu ◽  
Kexian Liang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probes ◽  
Caspase 3 ◽  
Near Infrared ◽  
Fluorescence Enhancement ◽  
Aqueous Media ◽  
Detection Sensitivity ◽  
Peptide Sequence ◽  
Nir Fluorescence ◽  
Fluorogenic Peptide

Background: Gold nanoparticles (AuNPs) are commonly used in nanomedicine because of their unique spectral properties, chemical and biological stability, and ability to quench the fluorescence of organic dyes attached to their surfaces. However, the utility of spherical AuNPs for activatable fluorescence sensing of molecular processes have been confined to resonance-matched fluorophores in the 500 nm to 600 nm spectral range to maximize dye fluorescence quenching efficiency. Expanding the repertoire of fluorophore systems into the NIR fluorescence regimen with emission >800 nm will facilitate the analysis of multiple biological events with high detection sensitivity. Objective: The primary goal of this study is to determine if spherical AuNP-induced radiative rate suppression of nonresonant near-infrared (NIR) fluorescent probes can serve as a versatile nanoconstruct for highly sensitive detection and imaging of activated caspase-3 in aqueous media and cancer cells. This required the development of activatable NIR fluorescence sensors of caspase-3 designed to overcome the nonspecific degradation and release of the surface coatings in aqueous media. Method: We harnessed the fluorescence-quenching properties and multivalency of spherical AuNPs to develop AuNPtemplated activatable NIR fluorescent probes to detect activated caspase-3, an intracellular reporter of early cell death. Freshly AuNPs were coated with a multifunctional NIR fluorescent dye-labeled peptide (LS422) consisting of an RGD peptide sequence that targets αvβ3-integrin protein (αvβ3) on the surface of cancer cells to mediate the uptake and internalization of the sensors in tumor cells; a DEVD peptide sequence for reporting the induction of cell death through caspase-3 mediated NIR fluorescence enhancement; and a multidentate hexacysteine sequence for enhancing selfassembly and stabilizing the multifunctional construct on AuNPs. The integrin binding affinity of LS422 and caspase-3 kinetics were determined by a radioligand competitive binding and fluorogenic peptide assays, respectively. Detection of intracellular caspase-3, cell viability, and the internalization of LS422 in cancer cells were determined by confocal NIR fluorescence spectroscopy and microscopy. Results: Narrow size AuNPs (13 nm) were prepared and characterized by transmission electron microscopy and dynamic light scattering. When assembled on the AuNPs, the binding constant of LS422 for αvβ3 improved 11-fold from 13.2 nM to 1.2 nM. Whereas the catalytic turnover of caspase-3 by LS422-AuNPs was similar to the reference fluorogenic peptide, the binding affinity for the enzyme increased by a factor of 2. Unlike the αvβ3 positive, but caspase-3 negative breast cancer MCF-7 cells, treatment of the αvβ3 and caspase-3 positive lung cancer A549 cells with Paclitaxel showed significant fluorescence enhancement within 30 minutes, which correlated with caspase-3 specific activation of LS422- AuNPs fluorescence. Incorporation of a 3.5 mW NIR laser source into our spectrofluorometer increased the detection sensitivity by an order of magnitude (limit of detection ~0.1 nM of cypate) and significantly decreased the signal noise relative to a xenon lamp. This gain in sensitivity enabled the detection of substrate hydrolysis at a broad range of inhibitor concentrations without photobleaching the cypate dye. Conclusion: The multifunctional AuNPs demonstrate the use of a non-resonant quenching strategy to design activatable NIR fluorescence molecular probes. The nanoconstruct offers a selective reporting method for detecting activated caspase-3, imaging of cell viability, identifying dying cells, and visualizing the functional status of intracellular enzymes. Performing these tasks with NIR fluorescent probes creates an opportunity to translate the in vitro and cellular analysis of enzymes into in vivo interrogation of their functional status using deep tissue penetrating NIR fluorescence analytical methods.

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