Spheroid culture of primary hepatocytes with short fibers as a predictable in vitro model for drug screening

2016 ◽  
Vol 4 (44) ◽  
pp. 7155-7167 ◽  
Author(s):  
Jiaojun Wei ◽  
Jinfu Lu ◽  
Yaowen Liu ◽  
Shili Yan ◽  
Xiaohong Li
Keyword(s):  
Drug Metabolism ◽  
Drug Screening ◽  
In Vitro Model ◽  
Short Fibers ◽  
Primary Hepatocytes ◽  
Spheroid Culture ◽  
Vitro Model ◽  
Hepatocyte Spheroids ◽  
Size Controlled

Short fibers are utilized as scaffolds for generation of size-controlled hepatocyte spheroids, exhibiting an efficient in vitro model for determining drug metabolism.

CRYOPRESERVED PRIMARY HEPATOCYTES AS A CONSTANTLY AVAILABLE IN VITRO MODEL FOR THE EVALUATION OF HUMAN AND ANIMAL DRUG METABOLISM AND ENZYME INDUCTION*

2000 ◽  
Vol 32 (1) ◽  
pp. 81-118 ◽  
Author(s):  
JAN G. HENGSTLER ◽  
D. UTESCH ◽  
P. STEINBERG ◽  
K. L. PLATT ◽  
B. DIENER ◽  
...  
Keyword(s):  
Drug Metabolism ◽  
Enzyme Induction ◽  
In Vitro Model ◽  
Primary Hepatocytes ◽  
Vitro Model

scholarly journals Crypt Organoid Culture as an In Vitro Model in Drug Metabolism and Cytotoxicity Studies

2017 ◽  
Vol 45 (7) ◽  
pp. 748-754 ◽  
Author(s):  
Wenqi Lu ◽  
Eva Rettenmeier ◽  
Miles Paszek ◽  
Mei-Fei Yueh ◽  
Robert H. Tukey ◽  
...  
Keyword(s):  
Drug Metabolism ◽  
In Vitro Model ◽  
Organoid Culture ◽  
Cytotoxicity Studies ◽  
Vitro Model

Hepatocyte spheroids as a viable in vitro model for recapitulation of complex in vivo metabolism pathways of loratadine in humans

Xenobiotica ◽  
2019 ◽  
Vol 50 (6) ◽  
pp. 621-629
Author(s):  
Silvi A. Chacko ◽  
Van T. Ly ◽  
Lisa J. Christopher ◽  
Jinping Gan
Keyword(s):  
In Vitro Model ◽  
In Vivo Metabolism ◽  
Vitro Model ◽  
Hepatocyte Spheroids

scholarly journals Microencapsulated multicellular tumor spheroids:preparation and use as a novel in vitro model for drug screening

2010 ◽  
Vol 56 (6) ◽  
pp. 674-685 ◽  
Author(s):  
A.M. Tsoy ◽  
D.S. Zaytseva-Zotova ◽  
E.F. Edelweiss ◽  
A. Bartkowiak ◽  
J-L. Goergen ◽  
...  
Keyword(s):  
Drug Screening ◽  
In Vitro Model ◽  
Monolayer Culture ◽  
Breast Adenocarcinoma ◽  
Viable Cells ◽  
Adenocarcinoma Cells ◽  
Mean Size ◽  
Vitro Model ◽  
Mcf 7

In the current study a technique for microencapsulation of human breast adenocarcinoma cells MCF-7 in alginate-chitosan microcapsules is used. Microencapsulation is proposed to generate multicellular tumor spheroids (MTS) based on these cells and to test them further as an in vitro model for anti-tumor drug screening. Cytotoxicity of methotrexate (MTX) was studied on the obtained MTS. A set of MTS with mean size of 150, 200 and 300 m was prepared in function of a cultivation time. After incubation of MTS in cultivation medium containing MTX at concentrations of 1, 2, 10, 50 and 100 nM for 48 hs cell viability was evaluated. MTS were shown to be more resistant to MTX than the monolayer culture, and the resistance to MTX was increased with enhancing a spheroid size. At MTX concentration of 100 nM a number of viable cells in MTS with the size of 300 m was 2.5-fold bigger than that one in monolayer culture. It is suggested that the cells in microencapsulated MTS can better mimic cell behavior in a small size solid tumor than the cells in a monolayer culture. In future microencapsulated MTS can be proposed as a novel in vitro model for anticancer drug screening.

scholarly journals DEVELOPMENT OF SECRETOR AND GESTATIONAL PATIENT-DERIVED ORGANOIDS FROM ADENOMYOSIS DISEASE AS AN IN VITRO MODEL TO STUDY ADENOMYOSIS-RELATED INFERTILITY AND PERSONALIZED DRUG SCREENING

2021 ◽  
Vol 116 (3) ◽  
pp. e310
Author(s):  
Elena Juárez-Barber ◽  
Ana Corachán ◽  
Emilio Francés-Herrero ◽  
Amparo Faus ◽  
Antonio Pellicer ◽  
...  
Keyword(s):  
Drug Screening ◽  
In Vitro Model ◽  
Vitro Model

Evaluation of HepaRG Cells as an in Vitro Model for Human Drug Metabolism Studies

2008 ◽  
Vol 36 (7) ◽  
pp. 1444-1452 ◽  
Author(s):  
Kajsa P. Kanebratt ◽  
Tommy B. Andersson
Keyword(s):  
Drug Metabolism ◽  
In Vitro Model ◽  
Heparg Cells ◽  
Vitro Model
Sign in / Sign up

Export Citation Format

Share Document