Paper-based FRET for the direct detection of collagen triple helix

2016 ◽  
Vol 4 (43) ◽  
pp. 7009-7013 ◽  
Author(s):  
Xiangdong Cai ◽  
Qiuxia Yang ◽  
Jiamin Ding ◽  
Weiran Ye ◽  
Xuan Li ◽  
...  
Keyword(s):  
Triple Helix ◽  
Direct Detection ◽  
Low Cost ◽  
Collagen Triple Helix ◽  
Multichannel Detection ◽  
Detection Mode ◽  
Collagen Molecules ◽  
Easy Operation ◽  
Patterned Paper

The integration of the GO-based FRET assay with a patterned paper provides a powerful new tool for the detection of collagen molecules with many superior features: tiny volumes of samples, multichannel detection mode, easy operation and low-cost equipment.

scholarly journals The Role of Collagen Triple Helix Repeat-Containing 1 Protein (CTHRC1) in Rheumatoid Arthritis

2021 ◽  
Vol 22 (5) ◽  
pp. 2426
Author(s):  
Askhat Myngbay ◽  
Limara Manarbek ◽  
Steve Ludbrook ◽  
Jeannette Kunz
Keyword(s):  
Rheumatoid Arthritis ◽  
Drug Targets ◽  
Triple Helix ◽  
Cancer Metastasis ◽  
Bone Erosion ◽  
Cartilage Destruction ◽  
Patient Treatment ◽  
Collagen Triple Helix ◽  
Potential Biomarker

Rheumatoid arthritis (RA) is a chronic autoimmune disease causing inflammation of joints, cartilage destruction and bone erosion. Biomarkers and new drug targets are actively sought and progressed to improve available options for patient treatment. The Collagen Triple Helix Repeat Containing 1 protein (CTHRC1) may have an important role as a biomarker for rheumatoid arthritis, as CTHRC1 protein concentration is significantly elevated in the peripheral blood of rheumatoid arthritis patients compared to osteoarthritis (OA) patients and healthy individuals. CTHRC1 is a secreted glycoprotein that promotes cell migration and has been implicated in arterial tissue-repair processes. Furthermore, high CTHRC1 expression is observed in many types of cancer and is associated with cancer metastasis to the bone and poor patient prognosis. However, the function of CTHRC1 in RA is still largely undefined. The aim of this review is to summarize recent findings on the role of CTHRC1 as a potential biomarker and pathogenic driver of RA progression. We will discuss emerging evidence linking CTHRC1 to the pathogenic behavior of fibroblast-like synoviocytes and to cartilage and bone erosion through modulation of the balance between bone resorption and repair.

scholarly journals A set of simple methods for detection and extraction of laminarinase

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ananthamurthy Koteshwara ◽  
Nancy V. Philip ◽  
Jesil Mathew Aranjani ◽  
Raghu Chandrashekhar Hariharapura ◽  
Subrahmanyam Volety Mallikarjuna
Keyword(s):  
Ammonium Sulfate ◽  
Ammonium Sulphate ◽  
Gold Standard ◽  
Direct Detection ◽  
Low Cost ◽  
Direct Analysis ◽  
Reducing Sugar ◽  
Ammonium Sulfate Precipitation ◽  
Simple Method ◽  
Functional Step

AbstractA carefully designed ammonium sulfate precipitation will simplify extraction of proteins and is considered to be a gold standard among various precipitation methods. Therefore, optimization of ammonium sulfate precipitation can be an important functional step in protein purification. The presence of high amounts of ammonium sulphate precludes direct detection of many enzymatically active proteins including reducing sugar assays (e.g. Nelson-Somogyi, Reissig and 3,5-dinitrosalicylic acid methods) for assessing carbohydrases (e.g. laminarinase (β (1–3)-glucanohydrolase), cellulases and chitinases). In this study, a simple method was developed using laminarin infused agarose plate for the direct analysis of the ammonium sulphate precipitates from Streptomyces rimosus AFM-1. The developed method is simple and convenient that can give accurate results even in presence of ammonium sulfate in the crude precipitates. Laminarin is a translucent substrate requiring the use of a stain to visualize the zones of hydrolysis in a plate assay. A very low-cost and locally available fluorescent optical fabric brightener Tinopal CBS-X has been used as a stain to detect the zones of hydrolysis. We also report simple methods to prepare colloidal chitin and cell free supernatant in this manuscript.

scholarly journals Operation strategy for a low-cost easy-operation Cassino Hexapod

2008 ◽  
Vol 4 (4) ◽  
pp. 149-156 ◽  
Author(s):  
Giuseppe Carbone ◽  
Aviral Shrot ◽  
Marco Ceccarelli
Keyword(s):  
Low Cost ◽  
Operation Strategy ◽  
Easy Operation

Comparative NMR Analysis of Collagen Triple Helix Organization from N- to C-Termini

2014 ◽  
Vol 16 (1) ◽  
pp. 145-155 ◽  
Author(s):  
Amanda M. Acevedo-Jake ◽  
Abhishek A. Jalan ◽  
Jeffrey D. Hartgerink
Keyword(s):  
Triple Helix ◽  
Nmr Analysis ◽  
Collagen Triple Helix

scholarly journals 5G Wireless/Wired Convergence of UFMC Based Modulation for Intensity Modulation Direct Detection

2021 ◽  
Author(s):  
Keerthivasan K ◽  
Shibu S
Keyword(s):  
Network Architecture ◽  
Direct Detection ◽  
Low Cost ◽  
Cost Effective ◽  
Theoretical Work ◽  
Base Stations ◽  
Complex Nature ◽  
Implementation Model ◽  
Effective Manner ◽  
Media Applications

Faster data speeds, shorter end-to-end latencies, improved end-user service efficiency, and a wider range of multi-media applications are expected with the new 5G wireless services. The dramatic increase in the number of base stations required to meet these criteria, which undermines the low-cost constraints imposed by operators, demonstrates the need for a paradigm shift in modern network architecture. Alternative formats will be required for next-generation architectures, where simplicity is the primary goal. The number of connections is expected to increase rapidly, breaking the inherent complexity of traditional coherent solutions and lowering the resulting cost percentage. A novel implementation model is used to migrate complex-nature modulation structures in a highly efficient and cost-effective manner. Theoretical work to analyses modulations’ behavior over a wired/fiber setup and wireless mode is also provided. The state-of-the-art computational complexity, simplicity, and ease of execution while maintaining efficiency throughput and bit error rate.

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