Pyrano[3,2-c]julolidin-2-ones: a novel class of fluorescent probes for ratiometric detection and imaging of Hg2+ in live cancer cells

2016 ◽  
Vol 4 (28) ◽  
pp. 4934-4940 ◽  
Author(s):  
Ajay Kumar Jha ◽  
Shahida Umar ◽  
Rakesh Kumar Arya ◽  
Dipak Datta ◽  
Atul Goel
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probes ◽  
Live Cells ◽  
Molecular Rotor ◽  
Ratiometric Detection

A novel pyrano[3,2-c]julolidin-2-one based fluorescent molecular rotor PYJO4 has been designed and developed for selective ratiometric detection, quantification and imaging of intracellular Hg2+ in live cells.

scholarly journals Cancer-Specific hNQO1-Responsive Biocompatible Naphthalimides Providing a Rapid Fluorescent Turn-On with an Enhanced Enzyme Affinity

Sensors ◽  
2019 ◽  
Vol 20 (1) ◽  
pp. 53 ◽  
Author(s):  
Sun Young Park ◽  
Eugeine Jung ◽  
Jong Seung Kim ◽  
Sung-Gil Chi ◽  
Min Hee Lee
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probes ◽  
Therapeutic Agent ◽  
Cell Imaging ◽  
Physiological Condition ◽  
Live Cells ◽  
Strong Fluorescence ◽  
Turn On ◽  
Efficient Detection ◽  
Agent Development

Human NAD(P)H:quinone oxidoreductase 1 (hNQO1) is overexpressed in cancer cells and associated with the drug resistance factor of cancer. The objective of this work is the development of fluorescent probes for the efficient detection of hNQO1 activity in cancer cells, which can be employed for the cancer diagnosis and therapeutic agent development. Herein, we report naphthalimide-based fluorescent probes 1 and 2 that can detect hNQO1. For hNQO1 activity, the probes showed a significant fluorescence increase at 540 nm. In addition, probe 1, the naphthalimide containing a triphenylphosphonium salt, showed an enhanced enzyme efficiency and rapid detection under a physiological condition. The detection ability of probe 1 was superior to that of other previously reported probes. Moreover, probe 1 was less cytotoxic during the cancer cell imaging and readily provided a strong fluorescence in hNQO1-overexpressed cancer cells (A549). We proposed that probe 1 can be used to detect hNQO1 expression in live cells and it will be applied to develop the diagnosis and customized treatment of hNQO1-related disease.

scholarly journals Fluorescent Probes for Live Cell Thiol Detection

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3575
Author(s):  
Shenggang Wang ◽  
Yue Huang ◽  
Xiangming Guan
Keyword(s):  
Fluorescent Probes ◽  
Biological System ◽  
High Sensitivity ◽  
Intracellular Distribution ◽  
Live Cell ◽  
Live Cells ◽  
High Demand ◽  
Non Invasive

Thiols play vital and irreplaceable roles in the biological system. Abnormality of thiol levels has been linked with various diseases and biological disorders. Thiols are known to distribute unevenly and change dynamically in the biological system. Methods that can determine thiols’ concentration and distribution in live cells are in high demand. In the last two decades, fluorescent probes have emerged as a powerful tool for achieving that goal for the simplicity, high sensitivity, and capability of visualizing the analytes in live cells in a non-invasive way. They also enable the determination of intracellular distribution and dynamitic movement of thiols in the intact native environments. This review focuses on some of the major strategies/mechanisms being used for detecting GSH, Cys/Hcy, and other thiols in live cells via fluorescent probes, and how they are applied at the cellular and subcellular levels. The sensing mechanisms (for GSH and Cys/Hcy) and bio-applications of the probes are illustrated followed by a summary of probes for selectively detecting cellular and subcellular thiols.

scholarly journals Serial profiling of cell-free DNA and nucleosome histone modifications in cell cultures

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Vida Ungerer ◽  
Abel J. Bronkhorst ◽  
Priscilla Van den Ackerveken ◽  
Marielle Herzog ◽  
Stefan Holdenrieder
Keyword(s):  
Cancer Cells ◽  
Histone Modifications ◽  
Chemical Properties ◽  
Basic Research ◽  
Live Cells ◽  
Cycle Phase ◽  
Cell Free Dna ◽  
Free Dna ◽  
High Resolution Analysis ◽  
Active Release

AbstractRecent advances in basic research have unveiled several strategies for improving the sensitivity and specificity of cell-free DNA (cfDNA) based assays, which is a prerequisite for broadening its clinical use. Included among these strategies is leveraging knowledge of both the biogenesis and physico-chemical properties of cfDNA towards the identification of better disease-defining features and optimization of methods. While good progress has been made on this front, much of cfDNA biology remains uncharted. Here, we correlated serial measurements of cfDNA size, concentration and nucleosome histone modifications with various cellular parameters, including cell growth rate, viability, apoptosis, necrosis, and cell cycle phase in three different cell lines. Collectively, the picture emerged that temporal changes in cfDNA levels are rather irregular and not the result of constitutive release from live cells. Instead, changes in cfDNA levels correlated with intermittent cell death events, wherein apoptosis contributed more to cfDNA release in non-cancer cells and necrosis more in cancer cells. Interestingly, the presence of a ~ 3 kbp cfDNA population, which is often deemed to originate from accidental cell lysis or active release, was found to originate from necrosis. High-resolution analysis of this cfDNA population revealed an underlying DNA laddering pattern consisting of several oligo-nucleosomes, identical to those generated by apoptosis. This suggests that necrosis may contribute significantly to the pool of mono-nucleosomal cfDNA fragments that are generally interrogated for cancer mutational profiling. Furthermore, since active steps are often taken to exclude longer oligo-nucleosomes from clinical biospecimens and subsequent assays this raises the question of whether important pathological information is lost.

A ratiometric fluorogenic probe for the real-time detection of SO32−in aqueous medium: application in a cellulose paper based device and potential to sense SO32−in mitochondria

The Analyst ◽  
2018 ◽  
Vol 143 (1) ◽  
pp. 250-257 ◽  
Author(s):  
Soham Samanta ◽  
Senjuti Halder ◽  
Poulomi Dey ◽  
Utsab Manna ◽  
Aiyagari Ramesh ◽  
...  
Keyword(s):  
Aqueous Medium ◽  
Real Time ◽  
Water Soluble ◽  
Live Cells ◽  
The Real ◽  
Cellulose Paper ◽  
Fluorogenic Probe ◽  
Ratiometric Detection ◽  
Real Time Detection

A new water soluble and fluorogenic probe (L) that can demonstrate the specific ratiometric detection of a SO2derivative (SO32−) in 100% aqueous medium and live cells has been designed and synthesized.

scholarly journals Overexpressed Pituitary Tumor-Transforming Gene Causes Aneuploidy in Live Human Cells

Endocrinology ◽  
2003 ◽  
Vol 144 (11) ◽  
pp. 4991-4998 ◽  
Author(s):  
Run Yu ◽  
Wenge Lu ◽  
Jiandong Chen ◽  
Chris J. McCabe ◽  
Shlomo Melmed
Keyword(s):  
Cancer Cells ◽  
Chromosome Segregation ◽  
Pituitary Tumor ◽  
Fluorescent Protein ◽  
Human Cancer ◽  
Live Cells ◽  
Pituitary Tumor Transforming Gene ◽  
Transforming Gene

Abstract The mammalian securin, pituitary tumor-transforming gene (PTTG), is overexpressed in several tumors and transforms cells in vitro and in vivo. To test the hypothesis that PTTG overexpression causes aneuploidy, enhanced green fluorescent protein (EGFP)-tagged PTTG (PTTG-EGFP) was expressed in human H1299 cancer cells (with undetectable endogenous PTTG expression) and mitosis of individual live cells observed. Untransfected cells and cells expressing EGFP alone exhibited appropriate mitosis. PTTG-EGFP markedly prolonged prophase and metaphase, indicating that PTTG blocks progression of mitosis to anaphase. In cells that underwent apparently normal mitosis (35 of 65 cells), PTTG-EGFP was degraded about 1 min before anaphase onset. Cells that failed to degrade PTTG-EGFP exhibited asymmetrical cytokinesis without chromosome segregation (18 of 65 cells) or chromosome decondensation without cytokinesis (9 of 65 cells), resulting in appearance of a macronucleus. Fifty-one of 55 cells expressing a nondegradable mutant PTTG exhibited asymmetrical cytokinesis without chromosome segregation, and some (4 of 55) decondensed chromosomes, both resulting in macronuclear formation. During this abnormal cytokinesis, all chromosomes and spindles and both centrosomes moved to one daughter cell, suggesting potential chaos in the subsequent mitosis. In conclusion, failure of PTTG degradation or enhanced PTTG accumulation, as a consequence of overexpression, inhibits mitosis progression and chromosome segregation but does not directly affect cytokinesis, resulting in aneuploidy. These results demonstrate that PTTG induces aneuploidy in single, live, human cancer cells.

scholarly journals Fluorescent Probes with Unnatural Amino Acids to Monitor Proteasome Activity in Real-Time

2020 ◽  
Author(s):  
Breanna L. Zerfas ◽  
Rachel A. Coleman ◽  
Andres Salazar Chaparro ◽  
Nathaniel J. Macatangay ◽  
Darci Trader
Keyword(s):  
Amino Acids ◽  
Small Molecule ◽  
Fluorescent Probes ◽  
Unnatural Amino Acids ◽  
Cell Types ◽  
Proteasome Activity ◽  
Live Cells ◽  
Fluorescent Substrate ◽  
The Individual ◽  
Small Molecule Modulators

<div> <div> <div> <p>The proteasome is an essential protein complex that, when dysregulated, can result in various diseases in eukaryotic cells. As such, understanding the enzymatic activity of the proteasome and what can alter it is crucial to elucidating its roles in these diseases. This can be done effectively by using activity-based fluorescent substrate probes, of which there are many commercially available that target the individual protease-like subunits in the 20S CP of the proteasome. Unfortunately, these probes have not displayed appropriate characteristics for their use in live cell-based assays. In the work presented here, we have developed a set of probes which have shown improved fluorescence properties and selectivity towards the proteasome compared to other cellular proteases. By including unnatural amino acids, we have found probes which can be utilized in various applications, including monitoring the effects of small molecule stimulators of the proteasome in live cells and comparing the relative proteasome activity across different cancer cell types. In future studies, we expect the fluorescent probes presented here will serve as tools to support the discovery and characterization of small molecule modulators of proteasome activity. </p> </div> </div> </div>

Harnessing Se=N to develop novel fluorescent probes for visualizing the variation of endogenous hypobromous acid (HOBr) during the administration of immunotherapeutic agent

2021 ◽  
Author(s):  
Jian Zhang ◽  
kaiqiang Liu ◽  
Jingwen Li ◽  
Yingying Xie ◽  
Yong Li ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Live Cells ◽  
Immunotherapeutic Agent ◽  
Hypobromous Acid ◽  
In Virtue Of

In virtue of the formation of Se=N, ABT-Se and NDI-Se were developed to detect and visualize endogenous HOBr in live cells. Specifically, the upregulation of HOBr was monitored by NDI-Se...

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