High mobility group box 1-immobilized nanofibrous scaffold enhances vascularization, osteogenesis and stem cell recruitment

2016 ◽  
Vol 4 (29) ◽  
pp. 5002-5014 ◽  
Author(s):  
Yonggang Lv ◽  
Chongwen Lin
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
High Mobility ◽  
Nanofibrous Scaffold ◽  
High Mobility Group ◽  
Bone Scaffold ◽  
Cell Recruitment ◽  
Trigger Signal

An engineered, multi-functional HMGB1-immobilized scaffold is developed. HMGB1 is immobilized on the surface of electrospinning poly-l-lactide (PLLA)/polycaprolactone (PCL) nanofibers via heparin, which is used as a “trigger” signal to make the bone scaffold capable of enhancing vascularization, inducing osteogenesis and recruiting stem cells.

High mobility group box 1 protein, a cue for stem cell recruitment

2004 ◽  
Vol 68 (6) ◽  
pp. 1165-1170 ◽  
Author(s):  
Roberta Palumbo ◽  
Marco E Bianchi
Keyword(s):  
Stem Cell ◽  
Protein A ◽  
High Mobility ◽  
High Mobility Group ◽  
Cell Recruitment

Stem cell recruitment based on scaffold features for bone tissue engineering

2021 ◽  
Author(s):  
Bin Xia ◽  
Yaxin Deng ◽  
Yonggang Lv ◽  
Guobao Chen
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Bone Formation ◽  
Chemical Modification ◽  
Bone Tissue Engineering ◽  
Bone Scaffold ◽  
New Bone Formation ◽  
Cell Recruitment ◽  
Physical And Chemical

Proper physical and chemical modification of a bone scaffold can effectively recruit endogenous stem cells to participate in the new bone formation.

scholarly journals The Mammalian High Mobility Group Protein AT-Hook 2 (HMGA2): Biochemical and Biophysical Properties, and Its Association with Adipogenesis

2020 ◽  
Vol 21 (10) ◽  
pp. 3710 ◽  
Author(s):  
Linjia Su ◽  
Zifang Deng ◽  
Fenfei Leng
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Dna Binding ◽  
High Mobility ◽  
High Mobility Group ◽  
Current Evidence ◽  
High Mobility Group Protein ◽  
Binding Motifs ◽  
Dna Binding Motifs ◽  
Group Protein

The mammalian high-mobility-group protein AT-hook 2 (HMGA2) is a small DNA-binding protein and consists of three “AT-hook” DNA-binding motifs and a negatively charged C-terminal motif. It is a multifunctional nuclear protein directly linked to obesity, human height, stem cell youth, human intelligence, and tumorigenesis. Biochemical and biophysical studies showed that HMGA2 is an intrinsically disordered protein (IDP) and could form homodimers in aqueous buffer solution. The “AT-hook” DNA-binding motifs specifically bind to the minor groove of AT-rich DNA sequences and induce DNA-bending. HMGA2 plays an important role in adipogenesis most likely through stimulating the proliferative expansion of preadipocytes and also through regulating the expression of transcriptional factor Peroxisome proliferator-activated receptor γ (PPARγ) at the clonal expansion step from preadipocytes to adipocytes. Current evidence suggests that a main function of HMGA2 is to maintain stemness and renewal capacity of stem cells by which HMGA2 binds to chromosome and lock chromosome into a specific state, to allow the human embryonic stem cells to maintain their stem cell potency. Due to the importance of HMGA2 in adipogenesis and tumorigenesis, HMGA2 is considered a potential therapeutic target for anticancer and anti-obesity drugs. Efforts are taken to identify inhibitors targeting HMGA2.

Regrow or Repair: An Update on Potential Regenerative Therapies for the Kidney

2021 ◽  
pp. ASN.2021081073
Author(s):  
Melissa Little ◽  
Benjamin Humphreys
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Kidney Development ◽  
Transcriptional Analysis ◽  
Cell Recruitment ◽  
Renal Stem Cells ◽  
Induced Pluripotent ◽  
Stem Cell Populations ◽  
A Site ◽  
Regenerative Therapies

Fifteen years ago, this journal published a review outlining future options for regenerating the kidney. At that time, stem cell populations were being identified in multiple tissues, the concept of stem cell recruitment to a site of injury was of great interest, and the possibility of postnatal renal stem cells was growing in momentum. Since that time, we have seen the advent of human induced pluripotent stem cells, substantial advances in our capacity to both sequence and edit the genome, global and spatial transcriptional analysis down to the single-cell level, and a pandemic that has challenged our delivery of health care to all. This article will look back over this period of time to see how our view of kidney development, disease, repair, and regeneration has changed and envision a future for kidney regeneration and repair over the next 15 years.

The High Mobility Group Protein HMGA2: A Co-Regulator of Chromatin Structure and Pluripotency in Stem Cells?

2009 ◽  
Vol 5 (3) ◽  
pp. 224-230 ◽  
Author(s):  
Kurt Pfannkuche ◽  
Heike Summer ◽  
Ou Li ◽  
Jürgen Hescheler ◽  
Peter Dröge
Keyword(s):  
Stem Cells ◽  
Chromatin Structure ◽  
High Mobility ◽  
High Mobility Group ◽  
High Mobility Group Protein ◽  
Group Protein

scholarly journals Fully reduced HMGB1 accelerates the regeneration of multiple tissues by transitioning stem cells to GAlert

2018 ◽  
Vol 115 (19) ◽  
pp. E4463-E4472 ◽  
Author(s):  
Geoffrey Lee ◽  
Ana Isabel Espirito Santo ◽  
Stefan Zwingenberger ◽  
Lawrence Cai ◽  
Thomas Vogl ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Cell Function ◽  
Elective Surgery ◽  
High Mobility ◽  
Underlying Mechanism ◽  
Clinical Scenarios ◽  
Stem And Progenitor Cells

A major discovery of recent decades has been the existence of stem cells and their potential to repair many, if not most, tissues. With the aging population, many attempts have been made to use exogenous stem cells to promote tissue repair, so far with limited success. An alternative approach, which may be more effective and far less costly, is to promote tissue regeneration by targeting endogenous stem cells. However, ways of enhancing endogenous stem cell function remain poorly defined. Injury leads to the release of danger signals which are known to modulate the immune response, but their role in stem cell-mediated repair in vivo remains to be clarified. Here we show that high mobility group box 1 (HMGB1) is released following fracture in both humans and mice, forms a heterocomplex with CXCL12, and acts via CXCR4 to accelerate skeletal, hematopoietic, and muscle regeneration in vivo. Pretreatment with HMGB1 2 wk before injury also accelerated tissue regeneration, indicating an acquired proregenerative signature. HMGB1 led to sustained increase in cell cycling in vivo, and using Hmgb1−/− mice we identified the underlying mechanism as the transition of multiple quiescent stem cells from G0 to GAlert. HMGB1 also transitions human stem and progenitor cells to GAlert. Therefore, exogenous HMGB1 may benefit patients in many clinical scenarios, including trauma, chemotherapy, and elective surgery.

scholarly journals Epigenetic Contribution of High-Mobility Group A Proteins to Stem Cell Properties

2018 ◽  
Vol 2018 ◽  
pp. 1-20 ◽  
Author(s):  
Vincenzo Giancotti ◽  
Natascha Bergamin ◽  
Palmina Cataldi ◽  
Claudio Rizzi
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Embryonic Stem ◽  
High Mobility ◽  
High Mobility Group ◽  
Dna Modification ◽  
Epigenetic Factors ◽  
Group A ◽  
Induced Pluripotent

High-mobility group A (HMGA) proteins have been examined to understand their participation as structural epigenetic chromatin factors that confer stem-like properties to embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and cancer stem cells (CSCs). The function of HMGA was evaluated in conjunction with that of other epigenetic factors such as histones and microRNAs (miRs), taking into consideration the posttranscriptional modifications (PTMs) of histones (acetylation and methylation) and DNA methylation. HMGA proteins were coordinated or associated with histone and DNA modification and the expression of the factors related to pluripotency. CSCs showed remarkable differences compared with ESCs and iPSCs.

scholarly journals Natural Killer (NK)/melanoma cell interaction induces NK-mediated release of chemotactic High Mobility Group Box-1 (HMGB1) capable of amplifying NK cell recruitment

2015 ◽  
Vol 4 (12) ◽  
pp. e1052353 ◽  
Author(s):  
Monica Parodi ◽  
Marco Pedrazzi ◽  
Claudia Cantoni ◽  
Monica Averna ◽  
Mauro Patrone ◽  
...  
Keyword(s):  
Natural Killer ◽  
Melanoma Cell ◽  
Nk Cell ◽  
High Mobility ◽  
Cell Interaction ◽  
High Mobility Group ◽  
Cell Recruitment
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