scholarly journals Catalytic activity of catalase–silica nanoparticle hybrids: from ensemble to individual entity activity

2017 ◽  
Vol 8 (3) ◽  
pp. 2303-2308 ◽  
Author(s):  
Crystal Chan ◽  
Lior Sepunaru ◽  
Stanislav V. Sokolov ◽  
Enno Kätelhön ◽  
Neil P. Young ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Electrochemical Detection ◽  
Silica Nanoparticle ◽  
Individual Entity

We demonstrate the electrochemical detection and characterization of individual nanoparticle–enzyme hybrids.

scholarly journals Microscopic Image Segmentation and Morphological Characterization of Novel Chitosan/Silica Nanoparticle/Nisin Films Using Antimicrobial Technique for Blueberry Preservation

Membranes ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 303
Author(s):  
Rokayya Sami ◽  
Schahrazad Soltane ◽  
Mahmoud Helal
Keyword(s):  
Ph Value ◽  
Silica Nanoparticle ◽  
Morphological Characterization ◽  
Aerobic Bacteria ◽  
Nano Materials ◽  
Elongation At Break ◽  
Stable Suspension ◽  
Average Size ◽  
Material Ductility

In the current work, the characterization of novel chitosan/silica nanoparticle/nisin films with the addition of nisin as an antimicrobial technique for blueberry preservation during storage is investigated. Chitosan/Silica Nanoparticle/N (CH-SN-N) films presented a stable suspension as the surface loads (45.9 mV) and the distribution was considered broad (0.62). The result shows that the pH value was increased gradually with the addition of nisin to 4.12, while the turbidity was the highest at 0.39. The content of the insoluble matter and contact angle were the highest for the Chitosan/Silica Nanoparticle (CH-SN) film at 5.68%. The use of nano-materials in chitosan films decreased the material ductility, reduced the tensile strength and elongation-at-break of the membrane. The coated blueberries with Chitosan/Silica Nanoparticle/N films reported the lowest microbial contamination counts at 2.82 log CFU/g followed by Chitosan/Silica Nanoparticle at 3.73 and 3.58 log CFU/g for the aerobic bacteria, molds, and yeasts population, respectively. It was observed that (CH) film extracted 94 regions with an average size of 449.10, at the same time (CH-SN) film extracted 169 regions with an average size of 130.53. The (CH-SN-N) film presented the best result at 5.19%. It could be observed that the size of the total region of the fruit for the (CH) case was the smallest (1663 pixels), which implied that the fruit lost moisture content. As a conclusion, (CH-SN-N) film is recommended for blueberry preservation to prolong the shelf-life during storage.

scholarly journals Synthesis, Characterization of Chitosan-Aluminum Oxide Nanocomposite for Green Synthesis of Annulated Imidazopyrazol Thione Derivatives

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1160
Author(s):  
Abir S. Abdel-Naby ◽  
Sara Nabil ◽  
Sarah Aldulaijan ◽  
Ibtisam M. Ababutain ◽  
Azzah I. Alghamdi ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Aluminum Oxide ◽  
Reaction Medium ◽  
Thiol Group ◽  
Docking Studies ◽  
Significant Loss ◽  
Gram Negative Bacteria ◽  
Organic Catalyst ◽  
Group 1

Chitosan-aluminum oxide nanocomposite was synthesized, characterized, and used as a green heterogeneous catalyst to synthesize novel imidazopyrazolylthione derivatives. Nanocomposite polymeric material was characterized by EDS-SEM and XRD. The powerful catalytic activity, and its base character of the nanocomposite, was used to synthesize imidazopyrazolylthione (1) in a good yield compared to traditional cyclocondensation synthesis. Using the nanocomposite catalyst, substitution of the thiol group (1) afforded the corresponding thiourea (2) and the corresponding ester (3). The efficiency of the nanocomposite over the traditional base organic catalyst, Et3N and NaOH, makes it an effective, economic, and reproducible nontoxic catalyst. Moreover, the heterogeneous nanocomposite polymeric film was easily isolated from the reaction medium, and recycled up to four times, without a significant loss of its catalytic activity. The newly synthesized derivatives were screened as antibacterial agents and showed high potency. Molecular docking was also performed for a more in-depth investigation. The results of the docking studies have demonstrated that the docked compounds have strong interaction energies with both Gram-positive and Gram-negative bacteria.

scholarly journals Tyrosinase Nanoparticles: Understanding the Melanogenesis Pathway by Isolating the Products of Tyrosinase Enzymatic Reaction

2021 ◽  
Vol 22 (2) ◽  
pp. 734
Author(s):  
Paul K. Varghese ◽  
Mones Abu-Asab ◽  
Emilios K. Dimitriadis ◽  
Monika B. Dolinska ◽  
George P. Morcos ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Large Scale ◽  
Magnetic Beads ◽  
Enzymatic Reaction ◽  
Oculocutaneous Albinism ◽  
Transmission Electron ◽  
Hill Kinetics ◽  
Rate Limiting ◽  
Human Tyrosinase

Human Tyrosinase (Tyr) is the rate-limiting enzyme of the melanogenesis pathway. Tyr catalyzes the oxidation of the substrate L-DOPA into dopachrome and melanin. Currently, the characterization of dopachrome-related products is difficult due to the absence of a simple way to partition dopachrome from protein fraction. Here, we immobilize catalytically pure recombinant human Tyr domain (residues 19–469) containing 6xHis tag to Ni-loaded magnetic beads (MB). Transmission electron microscopy revealed Tyr-MB were within limits of 168.2 ± 24.4 nm while the dark-brown melanin images showed single and polymerized melanin with a diameter of 121.4 ± 18.1 nm. Using Hill kinetics, we show that Tyr-MB has a catalytic activity similar to that of intact Tyr. The diphenol oxidase reactions of L-DOPA show an increase of dopachrome formation with the number of MB and with temperature. At 50 °C, Tyr-MB shows some residual catalytic activity suggesting that the immobilized Tyr has increased protein stability. In contrast, under 37 °C, the dopachrome product, which is isolated from Tyr-MB particles, shows that dopachrome has an orange-brown color that is different from the color of the mixture of L-DOPA, Tyr, and dopachrome. In the future, Tyr-MB could be used for large-scale productions of dopachrome and melanin-related products and finding a treatment for oculocutaneous albinism-inherited diseases.

Acoustic characterization of silica nanoparticle-impregnated Kevlar fabric

2021 ◽  
pp. 004051752110238
Author(s):  
Oluwafemi P Akinmolayan ◽  
James M Manimala
Keyword(s):  
Transmission Loss ◽  
Silica Nanoparticle ◽  
Air Gap ◽  
Thin Plates ◽  
Residual Porosity ◽  
Ballistic Performance ◽  
Number Of Layers ◽  
Structural Applications ◽  
Acoustic Characterization

Silica nanoparticle-impregnated Kevlar (SNK) fabric has better specific ballistic performance in comparison to its neat counterparts. For multifunctional structural applications using lightweight composites, combining this improved ballistic functionality with an acoustic functionality is desirable. In this study, acoustic characterization of neat and SNK samples is conducted using the normal-incidence impedance tube method. Both the absorption coefficient and transmission loss (TL) are measured in the 60–6000 Hz frequency range. The influence of parameters such as number of layers of neat or treated fabric, percentage by weight of nanoparticle addition, spacing between fabric layers, and residual porosity is examined. It is found that while absorption decreases with an increase in nanoparticle addition for frequencies above about 2500 Hz, increasing the number of layers shifts peak absorption to lower frequencies. By introducing an air-gap behind the fabric layer, dominant low-frequency (1000–3000 Hz) absorption peaks are obtained that correlate well with natural modes of mass-equivalent thin plates. Examining the influence of residual porosity by laminating the SNK samples reveals that it contributes to about 30–50% of the total absorption. Above about 1500 Hz, 3–5 dB of TL increase is obtained for SNK samples vis-à-vis the neat samples. TL is found to increase beyond that of the neat sample above a threshold frequency when an air-gap is introduced between two SNK layers. With an increase in the weight of nanoparticle addition, measured TL tends to be closer to mass law predictions. This study demonstrates that SNK fabric could provide improved acoustic performance in addition to its ballistic capabilities, making it suitable for multifunctional applications and could form the basis for the development of simplified models to predict the structural acoustic response of such nanoparticle–fabric composites.

Characterization of a novel carboxylesterase with catalytic activity toward di(2-ethylhexyl) phthalate from a soil metagenomic library

2021 ◽  
Vol 785 ◽  
pp. 147260
Author(s):  
Zhenzhen Yan ◽  
Liping Ding ◽  
Dandan Zou ◽  
Jiarong Qiu ◽  
Yuting Shao ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Metagenomic Library ◽  
Ethylhexyl Phthalate

Synthesis and characterization of highly ordered MCM-41 in an alkali-free system and its catalytic activity

1996 ◽  
Vol 38 (1-2) ◽  
pp. 33-37 ◽  
Author(s):  
Xiu S. Zhao ◽  
Gao Q. Lu ◽  
Graeme J. Millar ◽  
Xin S. Li
Keyword(s):  
Catalytic Activity ◽  
Synthesis And Characterization ◽  
Free System ◽  
Mcm 41

Synthesis and characterization of magnetically recyclable Ag nanoparticles immobilized on Fe3O4@C nanospheres with catalytic activity

2015 ◽  
Vol 335 ◽  
pp. 23-28 ◽  
Author(s):  
Wei-hong Li ◽  
Xiu-ping Yue ◽  
Chang-sheng Guo ◽  
Jia-pei Lv ◽  
Si-si Liu ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Ag Nanoparticles ◽  
Synthesis And Characterization
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