scholarly journals Design of a synthetic luminescent probe from a biomolecule binding domain: selective detection of AU-rich mRNA sequences

2017 ◽  
Vol 8 (2) ◽  
pp. 1658-1664 ◽  
Author(s):  
Laurent Raibaut ◽  
William Vasseur ◽  
Geoffrey D. Shimberg ◽  
Christine Saint-Pierre ◽  
Jean-Luc Ravanat ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Messenger Rna ◽  
Untranslated Region ◽  
Zinc Finger Protein ◽  
Binding Domain ◽  
Selective Detection ◽  
Luminescent Probe ◽  
Time Resolved ◽  
Luminescent Sensor ◽  
Finger Protein

We report the design of a luminescent sensor based upon the zinc finger protein TIS11d, that allows for the selective time-resolved detection of the UUAUUUAUU sequence of the 3′-untranslated region of messenger RNA.

scholarly journals A conserved CCCH-type zinc finger protein regulates mRNA nuclear adenylation and export

2009 ◽  
Vol 185 (2) ◽  
pp. 265-277 ◽  
Author(s):  
Jessica A. Hurt ◽  
Robert A. Obar ◽  
Bo Zhai ◽  
Natalie G. Farny ◽  
Steven P. Gygi ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Nuclear Export ◽  
Messenger Rna ◽  
Zinc Finger Protein ◽  
Liquid Chromatography Mass Spectrometry ◽  
Functional Conservation ◽  
Finger Protein ◽  
Interfering Rna ◽  
Processing Steps ◽  
Prior Processing

Coupling of messenger RNA (mRNA) nuclear export with prior processing steps aids in the fidelity and efficiency of mRNA transport to the cytoplasm. In this study, we show that the processes of export and polyadenylation are coupled via the Drosophila melanogaster CCCH-type zinc finger protein CG6694/dZC3H3 through both physical and functional interactions. We show that depletion of dZC3H3 from S2R+ cells results in transcript hyperadenylation. Using targeted coimmunoprecipitation and liquid chromatography mass spectrometry (MS)/MS techniques, we characterize interactions of known components of the mRNA nuclear export and polyadenylation machineries with dZC3H3. Furthermore, we demonstrate the functional conservation of this factor, as depletion of its human homologue ZC3H3 by small interfering RNA results in an mRNA export defect in human cells as well. Nuclear polyadenylated (poly(A)) RNA in ZC3H3-depleted cells is sequestered in foci removed from SC35-containing speckles, indicating a shift from the normal subnuclear distribution of poly(A) RNA. Our data suggest a model wherein ZC3H3 interfaces between the polyadenylation machinery, newly poly(A) mRNAs, and factors for transcript export.

scholarly journals Identification of Two Novel ACTH-Responsive Genes Encoding Manganese-Dependent Superoxide Dismutase (SOD2) and the Zinc Finger Protein TIS11b [Tetradecanoyl Phorbol Acetate (TPA)-Inducible Sequence 11b]

2002 ◽  
Vol 16 (6) ◽  
pp. 1417-1427 ◽  
Author(s):  
Anna M. Chinn ◽  
Delphine Ciais ◽  
Sabine Bailly ◽  
Edmond Chambaz ◽  
Jonathan LaMarre ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Zinc Finger ◽  
Untranslated Region ◽  
Zinc Finger Protein ◽  
Primary Cultures ◽  
Repetitive Sequences ◽  
Adrenocortical Function ◽  
Vegf Expression ◽  
Adrenocortical Cells ◽  
Finger Protein

Abstract ACTH is the major trophic factor regulating and maintaining adrenocortical function, affecting such diverse processes as steroidogenesis, cell proliferation, cell migration, and cell survival. We used differential display RT-PCR to identify genes that are rapidly induced by ACTH in the bovine adrenal cortex. Of 42 PCR products differentially amplified from primary cultures of bovine adrenocortical cells treated with 10 nm ACTH, six identified mRNAs that were confirmed by Northern blot analysis to be induced by ACTH. Four of these amplicons encoded noninformative repetitive sequences. Of the other two sequenced amplicons, one encoded a partial sequence for mitochondrial manganese-dependent superoxide dismutase (SOD2), an enzyme that is likely to protect adrenocortical cells from the cytotoxic effects of radical oxygen species generated during steroid biosynthesis. The second was identified as TIS11b (phorbol-12-myristate-13-acetate-inducible sequence 11b)/ERF-1/cMG, a member of the CCCH double-zinc finger protein family. SOD2 induction by ACTH was independent of extracellular steroid concentration or oxidative stress. SOD2 and TIS11b mRNA expressions were rapidly induced by ACTH, reaching a maximal level after 8 h and 3 h of treatment, respectively. These ACTH effects were mimicked by forskolin but appeared independent of cortisol secretion. Upon ACTH treatment, induction of TIS11b expression closely followed the previously characterized peak of vascular endothelial growth factor (VEGF) expression. Transfection of a TIS11b expression plasmid into 3T3 fibroblasts induced a decrease in the expression of a reporter gene placed upstream of the VEGF 3′-untranslated region, indicating that TIS11b may be an important regulator of VEGF expression through interaction with its 3′-untranslated region.

scholarly journals Characterization of the target DNA sequence for the DNA-binding domain of zinc finger protein 191

2008 ◽  
Vol 40 (8) ◽  
pp. 704-710 ◽  
Author(s):  
H. Wang ◽  
R. Sun ◽  
G. Liu ◽  
M. Yao ◽  
J. Fei ◽  
...  
Keyword(s):  
Dna Binding ◽  
Dna Sequence ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Finger Protein ◽  
Target Dna

scholarly journals Directing an artificial zinc finger protein to new targets by fusion to a non-DNA-binding domain

2015 ◽  
Vol 44 (7) ◽  
pp. 3118-3130 ◽  
Author(s):  
Wooi F. Lim ◽  
Jon Burdach ◽  
Alister P.W. Funnell ◽  
Richard C.M. Pearson ◽  
Kate G.R. Quinlan ◽  
...  
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
New Targets ◽  
Finger Protein ◽  
Artificial Zinc Finger Protein

scholarly journals Isolation of a cDNA clone encoding a zinc finger protein highly expressed in T-leukemia lines

Blood ◽  
1992 ◽  
Vol 80 (10) ◽  
pp. 2571-2576 ◽  
Author(s):  
BY Wu ◽  
EW Hanley ◽  
LA Turka ◽  
GJ Nabel
Keyword(s):  
Zinc Finger ◽  
Cdna Clone ◽  
Messenger Rna ◽  
Zinc Finger Protein ◽  
Leukemia Cell ◽  
Lymphoid Cells ◽  
Gap Gene ◽  
Finger Protein ◽  
Leukemia Cell Lines

Abstract A cDNA clone encoding a novel zinc finger protein expressed in lymphoid cells has been isolated. This protein contains 5 repeats of the C2H2 motif previously described in the Drosophila gap gene, Kruppel, which is involved in embryo segmentation. Northern blot analysis showed that the messenger RNA (mRNA) encoding this protein is expressed at high levels in a variety of T-leukemia cell lines, at lower levels in some B cells, but is not observed in nonlymphoid cells. Within the T lineage, the mRNA is found at high levels in both alpha beta and gamma delta T cells. These data suggest that this cDNA, designated Hkr-T1, represents a gene that may contribute to the determination of the differentiation and the specificity within lymphoid cells.

scholarly journals Persistent repression of tau in the brain using engineered zinc finger protein transcription factors

2021 ◽  
Vol 7 (12) ◽  
pp. eabe1611
Author(s):  
Susanne Wegmann ◽  
Sarah L. DeVos ◽  
Bryan Zeitler ◽  
Kimberly Marlen ◽  
Rachel E. Bennett ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Finger ◽  
Messenger Rna ◽  
Zinc Finger Protein ◽  
Neuronal Damage ◽  
Brain Diseases ◽  
Transcriptional Level ◽  
Finger Protein ◽  
Model Of Alzheimer’S Disease ◽  
Protein Transcription

Neuronal tau reduction confers resilience against β-amyloid and tau-related neurotoxicity in vitro and in vivo. Here, we introduce a novel translational approach to lower expression of the tau gene MAPT at the transcriptional level using gene-silencing zinc finger protein transcription factors (ZFP-TFs). Following a single administration of adeno-associated virus (AAV), either locally into the hippocampus or intravenously to enable whole-brain transduction, we selectively reduced tau messenger RNA and protein by 50 to 80% out to 11 months, the longest time point studied. Sustained tau lowering was achieved without detectable off-target effects, overt histopathological changes, or molecular alterations. Tau reduction with AAV ZFP-TFs was able to rescue neuronal damage around amyloid plaques in a mouse model of Alzheimer’s disease (APP/PS1 line). The highly specific, durable, and controlled knockdown of endogenous tau makes AAV-delivered ZFP-TFs a promising approach for the treatment of tau-related human brain diseases.

scholarly journals The MEP-1 zinc-finger protein acts with MOG DEAH box proteins to control gene expression via the fem-3 3??? untranslated region in Caenorhabditis elegans

RNA ◽  
2002 ◽  
Vol 8 (6) ◽  
pp. 725-739 ◽  
Author(s):  
MARCO BELFIORE ◽  
LAURA D. MATHIES ◽  
PAOLO PUGNALE ◽  
GARY MOULDER ◽  
ROBERT BARSTEAD ◽  
...  
Keyword(s):  
Gene Expression ◽  
Caenorhabditis Elegans ◽  
Zinc Finger ◽  
Untranslated Region ◽  
Zinc Finger Protein ◽  
Control Gene ◽  
Finger Protein ◽  
Control Gene Expression

scholarly journals Isolation of a cDNA clone encoding a zinc finger protein highly expressed in T-leukemia lines

Blood ◽  
1992 ◽  
Vol 80 (10) ◽  
pp. 2571-2576
Author(s):  
BY Wu ◽  
EW Hanley ◽  
LA Turka ◽  
GJ Nabel
Keyword(s):  
Zinc Finger ◽  
Cdna Clone ◽  
Messenger Rna ◽  
Zinc Finger Protein ◽  
Leukemia Cell ◽  
Lymphoid Cells ◽  
Gap Gene ◽  
Finger Protein ◽  
Leukemia Cell Lines

A cDNA clone encoding a novel zinc finger protein expressed in lymphoid cells has been isolated. This protein contains 5 repeats of the C2H2 motif previously described in the Drosophila gap gene, Kruppel, which is involved in embryo segmentation. Northern blot analysis showed that the messenger RNA (mRNA) encoding this protein is expressed at high levels in a variety of T-leukemia cell lines, at lower levels in some B cells, but is not observed in nonlymphoid cells. Within the T lineage, the mRNA is found at high levels in both alpha beta and gamma delta T cells. These data suggest that this cDNA, designated Hkr-T1, represents a gene that may contribute to the determination of the differentiation and the specificity within lymphoid cells.

A novel EVI1 gene family, MEL1, lacking a PR domain (MEL1S) is expressed mainly in t(1;3)(p36;q21)-positive AML and blocks G-CSF–induced myeloid differentiation

Blood ◽  
2003 ◽  
Vol 102 (9) ◽  
pp. 3323-3332 ◽  
Author(s):  
Ichiro Nishikata ◽  
Hidenori Sasaki ◽  
Mutsunori Iga ◽  
Yoko Tateno ◽  
Suzuko Imayoshi ◽  
...  
Keyword(s):  
Dna Binding ◽  
Zinc Finger ◽  
Myeloid Leukemia ◽  
Zinc Finger Protein ◽  
Binding Domain ◽  
Leukemia Cells ◽  
Dna Binding Domain ◽  
Consensus Sequences ◽  
Finger Protein ◽  
Pr Domain

Abstract We have identified a novel gene MEL1 (MDS1/EVI1-like gene 1) encoding a zinc finger protein near the breakpoint of t(1; 3)(p36;q21)-positive human acute myeloid leukemia (AML) cells. Here, we studied the structure, expression pattern, and function of MEL1 in leukemia cells. In this study, we have identified 3 transcription start sites, 1 in exon 1 and 2 in exon 2, and 2 kinds of translation products, 170 kDa (MEL1) and 150 kDa (MEL1S). Notably, the 150-kDa band of MEL1S was detected mainly in the t(1;3)(p36;q21)-positive AML cells. By immunoblot analysis and proteolytic mapping, it is suggested that the 150-kDa band of MEL1S in the leukemia cells is translated from the internal initiation codon ATG597 in exon 4 and is mostly lacking the amino-terminal PR domain of MEL1. By the cyclic amplification and selection of targets (CASTing) method for identifying consensus sequences, it was shown that the consensus sequences of MEL1 were included in 2 different consensus sequences for DNA-binding domain 1 and 2 (D1-CONS and D2-CONS) of EVI1. In reporter gene assays, MEL1S activated transcription via binding to D2-CONS; however, the fusion of MEL1 or MEL1S to GAL4 DNA-binding domain (DBD) made them GAL4 binding site–dependent transcriptional repressors. Moreover, overexpression of MEL1S blocked granulocytic differentiation induced by granulocyte colony-stimulating factor (G-CSF) in interleukin-3 (IL-3)–dependent murine myeloid L-G3 cells, while MEL1 could not block the differentiation. Thus, it is likely that overexpression of the zinc finger protein lacking the PR domain (EVI1 and MEL1S) in the leukemia cells is one of the causative factors in the pathogenesis of myeloid leukemia.

Sign in / Sign up

Export Citation Format

Share Document