scholarly journals MnO2nanosheet mediated “DD–A” FRET binary probes for sensitive detection of intracellular mRNA

2017 ◽  
Vol 8 (1) ◽  
pp. 668-673 ◽  
Author(s):  
Min Ou ◽  
Jin Huang ◽  
Xiaohai Yang ◽  
Ke Quan ◽  
Yanjing Yang ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Donor Acceptor ◽  
Fret Efficiency

The donor donor–acceptor (DD–A) FRET model has proven to have a higher FRET efficiency than donor–acceptor acceptor (D–AA), donor–acceptor (D–A), and donor donor–acceptor acceptor (DD–AA) FRET models.

A FRET aptasensor for sensitive detection of aflatoxin B1 based on a novel donor-acceptor pair between ZnS quantum dots and Ag nanocubes

2020 ◽  
Author(s):  
Chengquan Wang ◽  
Wuhao Zhang ◽  
Jing Qian ◽  
Li Wang ◽  
Yi Ren ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Aflatoxin B1 ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Sensitive Detection ◽  
Acceptor Pair ◽  
Donor Acceptor ◽  
Donor Acceptor Pair

Aflatoxin B1 (AFB1) is one of the most carcinogenic chemicals. We proposed a novel fluorescence resonance energy transfer (FRET) sensor based on aptamer recognition technology for the sensitive detection of...

scholarly journals Polymer-mediated ternary supramolecular interactions for sensitive detection of peptides

The Analyst ◽  
2017 ◽  
Vol 142 (1) ◽  
pp. 118-122 ◽  
Author(s):  
Mahalia A. C. Serrano ◽  
Huan He ◽  
Bo Zhao ◽  
Rajasekhar R. Ramireddy ◽  
Richard W. Vachet ◽  
...  
Keyword(s):  
Electrostatic Interactions ◽  
Sensitive Detection ◽  
Supramolecular Interactions ◽  
Supramolecular System ◽  
Donor Acceptor

A combination of donor–acceptor and electrostatic interactions in a three-component supramolecular system has been shown to form the basis for selective and sensitive detection of peptides.

scholarly journals Protein interaction quantified in vivo by spectrally resolved fluorescence resonance energy transfer

2004 ◽  
Vol 385 (1) ◽  
pp. 265-277 ◽  
Author(s):  
Valerică RAICU ◽  
David B. JANSMA ◽  
R. J. Dwayne MILLER ◽  
James D. FRIESEN
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Receptor Protein ◽  
Acceptor Pair ◽  
Donor Acceptor ◽  
Fret Efficiency ◽  
Fluorescence Resonance

We describe a fluorescence resonance energy transfer (FRET)-based method for finding in living cells the fraction of a protein population (αT) forming complexes, and the average number (n) of those protein molecules in each complex. The method relies both on sensitized acceptor emission and on donor de-quenching (by photobleaching of the acceptor molecules), coupled with full spectral analysis of the differential fluorescence signature, in order to quantify the donor/acceptor energy transfer. The approach and sensitivity limits are well suited for in vivo microscopic investigations. This is demonstrated using a scanning laser confocal microscope to study complex formation of the sterile 2 α-factor receptor protein (Ste2p), labelled with green, cyan, and yellow fluorescent proteins (GFP, CFP, and YFP respectively), in budding yeast Saccharomyces cerevisiae. A theoretical model is presented that relates the efficiency of energy transfer in protein populations (the apparent FRET efficiency, Eapp) to the energy transferred in a single donor/acceptor pair (E, the true FRET efficiency). We determined E by using a new method that relies on Eapp measurements for two donor/acceptor pairs, Ste2p–CFP/Ste2p–YFP and Ste2p–GFP/Ste2p–YFP. From Eapp and E we determined αT≈1 and n≈2 for Ste2 proteins. Since the Ste2p complexes are formed in the absence of the ligand in our experiments, we conclude that the α-factor pheromone is not necessary for dimerization.

scholarly journals Energy Transfer in Dendritic Systems Having Pyrene Peripheral Groups as Donors and Different Acceptor Groups

Polymers ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1062 ◽  
Author(s):  
Pasquale Porcu ◽  
Mireille Vonlanthen ◽  
Andrea Ruiu ◽  
Israel González-Méndez ◽  
Ernesto Rivera
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fullerene C60 ◽  
Energy Transfer Efficiency ◽  
Transfer Efficiency ◽  
Feature Article ◽  
Donor Acceptor ◽  
Fret Efficiency

In this feature article, a specific overview of resonance energy transfer (FRET) in dendritic molecules was performed. We focused mainly on constructs bearing peripheral pyrene groups as donor moieties using different acceptor groups, such as porphyrin, fullerene C60, ruthenium-bipyridine complexes, and cyclen-core. We have studied the effect of all the different donor-acceptor pairs in the energy transfer efficiency (FRET). In all cases, high FRET efficiency values were observed.

Water-Soluble Conjugated Polyelectrolytes with Molecular Bumper for Efficient FRET Biosensor

2006 ◽  
Vol 965 ◽  
Author(s):  
Han Young Woo ◽  
Doojin Vak ◽  
Guillermo C Bazan
Keyword(s):  
Photophysical Properties ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Structural Difference ◽  
Water Soluble ◽  
Conjugated Polyelectrolytes ◽  
Design Synthesis ◽  
Donor Acceptor ◽  
Thermodynamic Driving Force ◽  
Fret Efficiency

ABSTRACTWe report the design, synthesis and photophysical properties of two new water-soluble conjugated polyelectrolytes, poly(9,9'-bis(6-N,N,N-trimethylammoniumhexyl)fluorene-alt-1,4-(2,5-bis(6-N,N,N-trimethylammoniumhexyloxy))phenylene) tetrabromide (P1i) and poly((10,10'-bis(6-N,N,N-trimethylammoniumhexyl)-10H-spiro(anthracene-9,9'-fluorene))-alt-1,4-(2,5-bis(6-N,N,N-trimethylammoniumhexyloxy))phenylene) tetrabromide (P2i). They have same electronic conjugation but the main structural difference is the presence of the anthracenyl substituent orthogonal to the polymer main backbone in P2i. Fluorescence resonance energy transfer (FRET) from the two polymers as FRET donors to fluorescein-labeled single stranded DNA (ssDNA-Fl) was studied. It is observed the emission from ssDNA-Fl via FRET by excitation of P2i with a FRET efficiency of ∼60%. Fluorescein is not emissive within the ssDNA-Fl/P1i complex. We also observed clear PL quenching (Φ = 0.8 → Φ = 0.27) of fluorescein for P2i after electrostatic complexation with ssDNA-Fl. It suggests a quenching pathway of Fl emission through photo-induced charge transfer (PCT) in the electrostatic complex. Both P1i and P2i have same HOMO-LUMO electronic structures and a similar thermodynamic driving force for either FRET or PCT. It appears that PCT operates to a larger extent with P1i, proposing that the presence of the “molecular bumper” in P2i increases Fl emission by increasing the donor-acceptor distance, which decreases more acutely PCT quenching, relative to FRET.

Phenothiazine Based Donor-Acceptor Compounds with Solid-State Emission in the Yellow to NIR Region and Their Highly Selective and Sensitive Detection of Cyanide Ion in ppb Level

2018 ◽  
Vol 24 (43) ◽  
pp. 11042-11050 ◽  
Author(s):  
Elumalai Ramachandran ◽  
Somasundaram A. A. Vandarkuzhali ◽  
Gandhi Sivaraman ◽  
Raghavachari Dhamodharan
Keyword(s):  
Solid State ◽  
Sensitive Detection ◽  
Cyanide Ion ◽  
Donor Acceptor

Tuning of donor-acceptor linker in rhodamine-coumarin conjugates leads remarkable solvent dependent FRET efficiency for Al3+ imaging in HeLa cells

2016 ◽  
Vol 234 ◽  
pp. 222-230 ◽  
Author(s):  
Susanta Adhikari ◽  
Sandip Mandal ◽  
Avijit Ghosh ◽  
Subhajit Guria ◽  
Debasis Das
Keyword(s):  
Hela Cells ◽  
Donor Acceptor ◽  
Fret Efficiency
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