scholarly journals Specific protein labeling with caged fluorophores for dual-color imaging and super-resolution microscopy in living cells

2017 ◽  
Vol 8 (1) ◽  
pp. 559-566 ◽  
Author(s):  
Sebastian Hauke ◽  
Alexander von Appen ◽  
Tooba Quidwai ◽  
Jonas Ries ◽  
Richard Wombacher
Keyword(s):  
Mammalian Cells ◽  
Super Resolution ◽  
Specific Protein ◽  
Living Cells ◽  
Protein Labeling ◽  
Color Imaging ◽  
Dual Color ◽  
Resolution Imaging ◽  
Super Resolution Microscopy

We present new fluorophore-conjugates for dual-color photoactivation and super-resolution imaging inside live mammalian cells.

scholarly journals Super-resolution microscopy can identify specific protein distribution patterns in platelets incubated with cancer cells

Nanoscale ◽  
2019 ◽  
Vol 11 (20) ◽  
pp. 10023-10033 ◽  
Author(s):  
Jan Bergstrand ◽  
Lei Xu ◽  
Xinyan Miao ◽  
Nailin Li ◽  
Ozan Öktem ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Dictionary Learning ◽  
Super Resolution ◽  
Distribution Patterns ◽  
Specific Protein ◽  
Protein Distribution ◽  
Activated Platelets ◽  
Resolution Imaging ◽  
Super Resolution Microscopy

Super-resolution imaging of P-selectin in platelets together with dictionary learning allow specifically activated platelets to be identified in an automatic objective manner.

scholarly journals Cell‐Permeable Nanobodies Allow Dual‐Color Super‐Resolution Microscopy in Untransfected Living Cells

2021 ◽  
Author(s):  
Anselm Fabian Lowell Schneider ◽  
Laila Benz ◽  
Martin Lehmann ◽  
Christian Hackenberger
Keyword(s):  
Super Resolution ◽  
Living Cells ◽  
Dual Color ◽  
Super Resolution Microscopy

scholarly journals 3D super-resolved imaging in live cells using sub-diffractive plasmonic localization of hybrid nanopillar arrays

Nanophotonics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2847-2859
Author(s):  
Soojung Kim ◽  
Hyerin Song ◽  
Heesang Ahn ◽  
Seung Won Jun ◽  
Seungchul Kim ◽  
...  
Keyword(s):  
Signal To Noise Ratio ◽  
Imaging Techniques ◽  
Optical Loss ◽  
Super Resolution ◽  
Living Cells ◽  
Live Cells ◽  
Complex Biological System ◽  
Observation Volume ◽  
Resolution Imaging ◽  
Nanopillar Arrays

AbstractAnalysing dynamics of a single biomolecule using high-resolution imaging techniques has been had significant attentions to understand complex biological system. Among the many approaches, vertical nanopillar arrays in contact with the inside of cells have been reported as a one of useful imaging applications since an observation volume can be confined down to few-tens nanometre theoretically. However, the nanopillars experimentally are not able to obtain super-resolution imaging because their evanescent waves generate a high optical loss and a low signal-to-noise ratio. Also, conventional nanopillars have a limitation to yield 3D information because they do not concern field localization in z-axis. Here, we developed novel hybrid nanopillar arrays (HNPs) that consist of SiO2 nanopillars terminated with gold nanodisks, allowing extreme light localization. The electromagnetic field profiles of HNPs are obtained through simulations and imaging resolution of cell membrane and biomolecules in living cells are tested using one-photon and 3D multiphoton fluorescence microscopy, respectively. Consequently, HNPs present approximately 25 times enhanced intensity compared to controls and obtained an axial and lateral resolution of 110 and 210 nm of the intensities of fluorophores conjugated with biomolecules transported in living cells. These structures can be a great platform to analyse complex intracellular environment.

De Novo-Designed Landmine Warfare Strategy Luminophore for Super-resolution Imaging Reveal ONOO- evolution in Living Cells

2021 ◽  
pp. 130151
Author(s):  
Yuanyuan Liu ◽  
Chengying Zhang ◽  
Yongchun Wei ◽  
Huimin Chen ◽  
Lingxiu Kong ◽  
...  
Keyword(s):  
De Novo ◽  
Super Resolution ◽  
Living Cells ◽  
Resolution Imaging

scholarly journals Combined Use of Unnatural Amino Acids Enables Dual-Color Super-Resolution Imaging of Proteins via Click Chemistry

ACS Nano ◽  
2018 ◽  
Vol 12 (12) ◽  
pp. 12247-12254 ◽  
Author(s):  
Kim-A. Saal ◽  
Frank Richter ◽  
Peter Rehling ◽  
Silvio O. Rizzoli
Keyword(s):  
Amino Acids ◽  
Click Chemistry ◽  
Unnatural Amino Acids ◽  
Super Resolution ◽  
Dual Color ◽  
Combined Use ◽  
Resolution Imaging

scholarly journals Super-Resolution Imaging by Dual Iterative Structured Illumination Microscopy

2021 ◽  
Author(s):  
Anna Loeschberger ◽  
Yauheni Novikau ◽  
Ralf Netz ◽  
Marie-Christin Spindler ◽  
Ricardo Benavente ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Brain Slices ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
Living Cells ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Spatiotemporal Resolution ◽  
Coated Pits ◽  
Resolution Imaging

Three-dimensional (3D) multicolor super-resolution imaging in the 50-100 nm range in fixed and living cells remains challenging. We extend the resolution of structured illumination microscopy (SIM) by an improved nonlinear iterative reconstruction algorithm that enables 3D multicolor imaging with improved spatiotemporal resolution at low illumination intensities. We demonstrate the performance of dual iterative SIM (diSIM) imaging cellular structures in fixed cells including synaptonemal complexes, clathrin coated pits and the actin cytoskeleton with lateral resolutions of 60-100 nm with standard fluorophores. Furthermore, we visualize dendritic spines in 70 micrometer thick brain slices with an axial resolution < 200 nm. Finally, we image dynamics of the endoplasmatic reticulum and microtubules in living cells with up to 255 frames/s.

Dual-color fluorescence emission difference super-resolution microscopy

2018 ◽  
Vol 11 (3) ◽  
pp. 329-336
Author(s):  
张智敏 ZHANG Zhi-min ◽  
匡翠方 KUANG Cui-fang ◽  
王子昂 WANG Zi-ang ◽  
朱大钊 ZHU Da-zhao ◽  
陈友华 CHEN You-hua ◽  
...  
Keyword(s):  
Fluorescence Emission ◽  
Super Resolution ◽  
Dual Color ◽  
Super Resolution Microscopy
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