scholarly journals A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva

2017 ◽  
Vol 8 (1) ◽  
pp. 131-141 ◽  
Author(s):  
Haixiang Yu ◽  
Juan Canoura ◽  
Bhargav Guntupalli ◽  
Xinhui Lou ◽  
Yi Xiao
Keyword(s):  
Fluorescence Detection ◽  
Signal Amplification ◽  
Rapid Detection ◽  
Fluorescence Assay ◽  
Cooperative Binding ◽  
Binding Mechanism ◽  
One Step ◽  
Aptamer Assay ◽  
Target Binding

A fluorescence assay based on a split aptamer featuring a cooperative-target-binding mechanism performs one-step, rapid detection of as low as 50 nM in 10% saliva without signal amplification.

A light-up fluorescence assay for tumor cell detection based on bifunctional split aptamers

The Analyst ◽  
2018 ◽  
Vol 143 (15) ◽  
pp. 3579-3585 ◽  
Author(s):  
Yuqiong Sun ◽  
Baoyin Yuan ◽  
Meitao Deng ◽  
Qing Wang ◽  
Jin Huang ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Fluorescence Detection ◽  
Fluorescence Assay ◽  
Cell Detection ◽  
Label Free ◽  
Tumor Cell Detection ◽  
One Step

Truncating, splitting and fusing of two aptamers for label-free and one-step fluorescence detection of tumor cells.

A dual signal amplification strategy for the highly sensitive fluorescence detection of nucleic acids

The Analyst ◽  
2020 ◽  
Vol 145 (4) ◽  
pp. 1219-1226 ◽  
Author(s):  
Jingjing Zhang ◽  
Chunyuan Song ◽  
Huiling Zhou ◽  
Juan Jia ◽  
Yinna Dai ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Fluorescence Detection ◽  
Signal Amplification ◽  
Fluorescence Assay ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Dual Signal Amplification

A dual signal amplification strategy comprising target-triggered recycling and DSN-mediated amplifications was designed and proposed for a highly sensitive fluorescence assay of nucleic acids.

scholarly journals Development and validation of a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of ZIKV in patient samples from Brazil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Severino Jefferson Ribeiro da Silva ◽  
Keith Pardee ◽  
Udeni B. R. Balasuriya ◽  
Lindomar Pena
Keyword(s):  
Reverse Transcription ◽  
Rapid Detection ◽  
Quantitative Polymerase Chain Reaction ◽  
Rna Extraction ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Serum Samples ◽  
Low Resource ◽  
Loop Mediated Isothermal Amplification ◽  
One Step

AbstractWe have previously developed and validated a one-step assay based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of the Zika virus (ZIKV) from mosquito samples. Patient diagnosis of ZIKV is currently carried out in centralized laboratories using the reverse transcription-quantitative polymerase chain reaction (RT-qPCR), which, while the gold standard molecular method, has several drawbacks for use in remote and low-resource settings, such as high cost and the need of specialized equipment. Point-of-care (POC) diagnostic platforms have the potential to overcome these limitations, especially in low-resource countries where ZIKV is endemic. With this in mind, here we optimized and validated our RT-LAMP assay for rapid detection of ZIKV from patient samples. We found that the assay detected ZIKV from diverse sample types (serum, urine, saliva, and semen) in as little as 20 min, without RNA extraction. The RT-LAMP assay was highly specific and up to 100 times more sensitive than RT-qPCR. We then validated the assay using 100 patient serum samples collected from suspected cases of arbovirus infection in the state of Pernambuco, which was at the epicenter of the last Zika epidemic. Analysis of the results, in comparison to RT-qPCR, found that the ZIKV RT-LAMP assay provided sensitivity of 100%, specificity of 93.75%, and an overall accuracy of 95.00%. Taken together, the RT-LAMP assay provides a straightforward and inexpensive alternative for the diagnosis of ZIKV from patients and has the potential to increase diagnostic capacity in ZIKV-affected areas, particularly in low and middle-income countries.

MGB-based one-step multiplex real-time PCR method for rapid detection of HPV

2013 ◽  
Vol 12 (3) ◽  
pp. 107-113 ◽  
Author(s):  
Jie Huang ◽  
Chun Gao ◽  
Xilai Ding ◽  
Shoufang Qu ◽  
Licheng Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
One Step ◽  
Pcr Method

scholarly journals A One-Step, Real-Time PCR Assay for Rapid Detection of Rhinovirus

2010 ◽  
Vol 12 (1) ◽  
pp. 102-108 ◽  
Author(s):  
Duc H. Do ◽  
Stella Laus ◽  
Amy Leber ◽  
Mario J. Marcon ◽  
Jeanne A. Jordan ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
One Step

Target-activated cascaded digestion amplification of exonuclease III aided signal-on and ultrasensitive fluorescence detection of ATP

2018 ◽  
Vol 42 (5) ◽  
pp. 3534-3540 ◽  
Author(s):  
Xueqi Leng ◽  
Rongguo Li ◽  
Yu Wang ◽  
Yunping Wu ◽  
Yuqin Tu ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Fluorescence Detection ◽  
Fluorescence Sensing ◽  
Exonuclease Iii ◽  
One Step ◽  
Exo Iii

In this work, a rapid, one-step and ultrasensitive signal-on fluorescence sensing for the detection of adenosine triphosphate (ATP) based on target-activated cascaded digestion amplification with Exo III aid was developed.

Rapid detection and cellular fluorescence imaging of the TBI biomarker Let-7i using a DNA–AgNC nanoprobe

2019 ◽  
Vol 43 (21) ◽  
pp. 7997-8004 ◽  
Author(s):  
Xingmei Li ◽  
Leiming Han ◽  
Yadong Guo ◽  
Yunfeng Chang ◽  
Jie Yan ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescence Detection ◽  
Rapid Detection ◽  
Intracellular Imaging

Rapid fluorescence detection of Let-7i for TBI diagnosis and intracellular imaging have been studied using the multifunctional DNA–AgNCs.

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