scholarly journals Dual-channel NIR activatable theranostic prodrug for in vivo spatiotemporal tracking thiol-triggered chemotherapy

2016 ◽  
Vol 7 (8) ◽  
pp. 4958-4965 ◽  
Author(s):  
Mingzhou Ye ◽  
Xiaohang Wang ◽  
Jianbin Tang ◽  
Zhiqian Guo ◽  
Youqing Shen ◽  
...  
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Release Profile ◽  
Fluorescent Imaging ◽  
Disulfide Linkage ◽  
Dual Channel ◽  
Nir Fluorescence ◽  
Real Time Tracking ◽  
First Time

Real-time tracking of where, when, and how prodrugs are established. A novel theranostic prodrug based on the disulfide linkage with two distinct switchable near-infrared (NIR) fluorescence can precisely extract the prodrug release profilein vivothrough dual-channel fluorescent imaging for the first time.

scholarly journals Dual-channel near-infrared fluorescent thiol-activatable theranostic prodrug for in vivo real-time tracking chemotherapy

2016 ◽  
Vol 4 ◽  
Author(s):  
Tang Jianbin ◽  
Ye Minghzou ◽  
Wang Xiaohang ◽  
Guo Zhiqian ◽  
Shen Youqing ◽  
...  
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Dual Channel ◽  
Real Time Tracking

A selective and sensitive near-infrared fluorescent probe for in vivo real time tracking of exogenous and metabolized hydrazine, a genotoxic impurity

2020 ◽  
Vol 8 (45) ◽  
pp. 10353-10359
Author(s):  
Shun Wang ◽  
Jian Liu ◽  
Linjiang Song ◽  
Qingrong Qi ◽  
Zicheng Li ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Real Time Tracking

The hydrazine level in the liver and kidneys of mice after administration of isoniazid was monitored by using probe Hcy-DB.

Photocaged prodrug under NIR light-triggering with dual-channel fluorescence: in vivo real-time tracking for precise drug delivery

2018 ◽  
Vol 61 (10) ◽  
pp. 1293-1300 ◽  
Author(s):  
Zhiqian Guo ◽  
Yaguang Ma ◽  
Yajing Liu ◽  
Chenxu Yan ◽  
Ping Shi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Real Time ◽  
Dual Channel ◽  
Nir Light ◽  
Real Time Tracking

scholarly journals Shortwave infrared fluorescence imaging with the clinically approved near-infrared dye indocyanine green

2018 ◽  
Vol 115 (17) ◽  
pp. 4465-4470 ◽  
Author(s):  
Jessica A. Carr ◽  
Daniel Franke ◽  
Justin R. Caram ◽  
Collin F. Perkinson ◽  
Mari Saif ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Emission Spectra ◽  
Nir Fluorescence ◽  
Near Infrared Dye ◽  
Silicon Based ◽  
Shortwave Infrared

Fluorescence imaging is a method of real-time molecular tracking in vivo that has enabled many clinical technologies. Imaging in the shortwave IR (SWIR; 1,000–2,000 nm) promises higher contrast, sensitivity, and penetration depths compared with conventional visible and near-IR (NIR) fluorescence imaging. However, adoption of SWIR imaging in clinical settings has been limited, partially due to the absence of US Food and Drug Administration (FDA)-approved fluorophores with peak emission in the SWIR. Here, we show that commercially available NIR dyes, including the FDA-approved contrast agent indocyanine green (ICG), exhibit optical properties suitable for in vivo SWIR fluorescence imaging. Even though their emission spectra peak in the NIR, these dyes outperform commercial SWIR fluorophores and can be imaged in the SWIR, even beyond 1,500 nm. We show real-time fluorescence imaging using ICG at clinically relevant doses, including intravital microscopy, noninvasive imaging in blood and lymph vessels, and imaging of hepatobiliary clearance, and show increased contrast compared with NIR fluorescence imaging. Furthermore, we show tumor-targeted SWIR imaging with IRDye 800CW-labeled trastuzumab, an NIR dye being tested in multiple clinical trials. Our findings suggest that high-contrast SWIR fluorescence imaging can be implemented alongside existing imaging modalities by switching the detection of conventional NIR fluorescence systems from silicon-based NIR cameras to emerging indium gallium arsenide-based SWIR cameras. Using ICG in particular opens the possibility of translating SWIR fluorescence imaging to human clinical applications. Indeed, our findings suggest that emerging SWIR-fluorescent in vivo contrast agents should be benchmarked against the SWIR emission of ICG in blood.

Dual-channel near-infrared fluorescent probe for real-time tracking of endogenous γ-glutamyl transpeptidase activity

2018 ◽  
Vol 54 (87) ◽  
pp. 12393-12396 ◽  
Author(s):  
Bing Bai ◽  
Chenxu Yan ◽  
Yutao Zhang ◽  
Zhiqian Guo ◽  
Wei-Hong Zhu
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Dual Channel ◽  
Nir Imaging ◽  
Real Time Tracking ◽  
Glutamyl Transpeptidase

We developed a curcuminoid difluoroboron-based fluorescent probe for tracking endogenous GGT activity with dual-channel light-up near-infrared (NIR) imaging.

scholarly journals Differential responses of transplanted stem cells to the diseased environment unveiled by a single molecular NIR II cell tracker

2020 ◽  
Author(s):  
Hao Chen ◽  
Huaxiao Yang ◽  
Chen Zhang ◽  
Si Chen ◽  
Xin Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Therapy ◽  
Single Cell ◽  
Real Time ◽  
Cell Cluster ◽  
Real Time Tracking ◽  
Cluster Resolution ◽  
First Time

AbstractStem cell therapy holds high promises in regenerative medicine. The major challenge of clinical translation is to precisely and quantitatively evaluate the in vivo cell distribution, migration, and engraftment, which cannot be easily achieved by current techniques. To address this issue, for the first time, we have developed a single molecular cell tracker with a strong fluorescence signal in the second near-infrared (NIR-II) window (1000-1700 nm) for real-time monitoring of in vivo cell behaviors in both healthy and diseased animal models. The NIR-II tracker (CelTrac1000) has shown complete cell labeling with low cytotoxicity and profound long-term tracking ability for 30 days in high temporospatial resolution for semi-quantification of the biodistribution of primary mesenchymal stem cell and induced pluripotent stem cell-derived endothelial cells. Taking advantage of the unique merits of CelTrac1000, the responses of transplanted stem cells to different diseased environments have been discriminated and unveiled. Furthermore, we also demonstrate CelTrac1000 as a universal and effective technique for ultrafast real-time tracking of the cellular migration and distribution in a single cell cluster resolution, along with the lung contraction and heart beating. As such, this single molecular NIR-II tracker will shift the optical cell tracking into a single cell cluster and millisecond temporospatial resolution for better evaluating and understanding stem cell therapy, affording optimal doses and efficacy.Significance StatementFor the first time, we synthesized a NIR-II tracker (CelTrac1000) for ultrafast real-time tracking of the migration trajectory of transplanted mesenchymal stem cells in the circulatory system with a single cell cluster resolution. Taking advantage of the merits of CelTrac1000, the responses of transplanted stem cells to different diseased environments, including acute lung injury, myocardial infarction, and middle cerebral artery occlusion, have been discriminated and unveiled in mice models. As such, our approach can help correlate critical biomedical information in stem cell therapies, such as stem cell dosing and engraftment and their relationships with efficacy, providing more accurate therapeutic treatment and outcomes in certain diseases during a long evaluation period (>30 days) in comparison with the commercial Qtracker (7-10 days).

scholarly journals Molecularly precise self-assembly of theranostic nanoprobes within a single-molecular framework for in vivo tracking of tumor-specific chemotherapy

2018 ◽  
Vol 9 (22) ◽  
pp. 4959-4969 ◽  
Author(s):  
Chenxu Yan ◽  
Zhiqian Guo ◽  
Yanyan Shen ◽  
Yi Chen ◽  
He Tian ◽  
...  
Keyword(s):  
Real Time ◽  
Self Assembly ◽  
Real Time Tracking ◽  
First Time ◽  
Molecular Framework

The strategy of molecularly precise self-assembly of theranostic nanoprobes within a single-molecular framework is used to avoid batch-to-batch variability, and concurrently achieving real-time tracking of the in vivo behaviour of prodrugs for the first time.

In vivo ratiometric tracking of endogenous β-galactosidase activity using an activatable near-infrared fluorescent probe

2019 ◽  
Vol 55 (82) ◽  
pp. 12308-12311 ◽  
Author(s):  
Limin Shi ◽  
Chenxu Yan ◽  
Yiyu Ma ◽  
Ting Wang ◽  
Zhiqian Guo ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Nude Mice ◽  
Near Infrared ◽  
Living Cells ◽  
Galactosidase Activity ◽  
Tumor Bearing ◽  
Real Time Tracking

Herein, we exemplify BODIPY-βgal as a ratiometric light-up NIR probe for the quantitative sensing of endogenous β-gal distribution in living cells, and real-time tracking of β-gal activity in tumor-bearing nude mice.

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