scholarly journals Synthesis, characterization and programmable toxicity of iron oxide nanoparticles conjugated withd-amino acid oxidase

RSC Advances ◽  
2017 ◽  
Vol 7 (3) ◽  
pp. 1439-1442 ◽  
Author(s):  
Riccardo Balzaretti ◽  
Fabian Meder ◽  
Marco P. Monopoli ◽  
Luca Boselli ◽  
Ilaria Armenia ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Amino Acid ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Acid Oxidase ◽  
Ros Production ◽  
Amino Acid Oxidase ◽  
Low Toxic

RgDAAO conjugated to γ-Fe2O3NPs generates a low toxic NP-DAAO system, which kills cancer cells through ROS production.

Polymer Coated Iron Nanoparticles: Radiolabeling & In Vitro Studies

2020 ◽  
Vol 13 ◽  
Author(s):  
Selin Yılmaz ◽  
Çiğdem İçhedef ◽  
Kadriye Buşra Karatay ◽  
Serap Teksöz
Keyword(s):  
Breast Cancer ◽  
Drug Delivery ◽  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Breast Cancer Cells ◽  
Cancer Drug ◽  
Oxide Nanoparticles ◽  
Sem Images

Backgorund: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it’s aimed to develop a novel targeted 99mTc radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with 99mTc. SPIONs were synthesized by reduction–coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were caharacterized by dynamic light scattering(DLS)and scanning electron microscopy(SEM), respectively. Radiolabeling yield of SPION-PLGAGEM nanoparticles were determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles were determined as 366.6 nm by DLS, while zeta potential was found as-29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4±0.16 % by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles were determined as 97.8±1.75 % via TLRC. Cytotoxicity of GEM loaded SPION-PLGA were investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, whilst incorporation rate was increased for both cell lines which external magnetic field application. Conclusion: 99mTc labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and supermagnetic characteristics.

Evaluation of Surface-modified Superparamagnetic Iron Oxide Nanoparticles to Optimize Bacterial Immobilization for Bio-separation with the Least Inhibitory Effect on Microorganism Activity

2020 ◽  
Vol 10 (2) ◽  
pp. 166-174
Author(s):  
Mehdi Khoshneviszadeh ◽  
Sarah Zargarnezhad ◽  
Younes Ghasemi ◽  
Ahmad Gholami
Keyword(s):  
Iron Oxide ◽  
Amino Acid ◽  
Magnetic Nanoparticles ◽  
Iron Oxide Nanoparticles ◽  
Surface Coating ◽  
Superparamagnetic Iron Oxide ◽  
Superparamagnetic Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Surface Charges ◽  
Surface Modified

Background: Magnetic cell immobilization has been introduced as a novel, facile and highly efficient approach for cell separation. A stable attachment between bacterial cell wall with superparamagnetic iron oxide nanoparticles (SPIONs) would enable the microorganisms to be affected by an outer magnetic field. At high concentrations, SPIONs produce reactive oxygen species in cytoplasm, which induce apoptosis or necrosis in microorganisms. Choosing a proper surface coating could cover the defects and increase the efficiency. Methods: In this study, asparagine, APTES, lipo-amino acid and PEG surface modified SPIONs was synthesized by co-precipitation method and characterized by FTIR, TEM, VSM, XRD, DLS techniques. Then, their protective effects against four Gram-positive and Gram-negative bacterial strains including Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were examined through microdilution broth and compared to naked SPION. Results: The evaluation of characterization results showed that functionalization of magnetic nanoparticles could change their MS value, size and surface charges. Also, the microbial analysis revealed that lipo-amino acid coated magnetic nanoparticles has the least adverse effect on microbial strain among tested SPIONs. Conclusion: This study showed lipo-amino acid could be considered as the most protective and even promotive surface coating, which is explained by its optimizing effect on cell penetration and negligible reductive effects on magnetic properties of SPIONs. lipo-amino acid coated magnetic nanoparticles could be used in microbial biotechnology and industrial microbiology.

scholarly journals The anti-ovarian carcinoma activity of L-amino Acid Oxidase from Crotalus adamanteus venom in vivo and in vitro

2021 ◽  
Author(s):  
Li Bo ◽  
Yan Xiong ◽  
Qiyi He ◽  
Xiaodong Yu ◽  
Bo Li ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Amino Acid ◽  
Cancer Cells ◽  
Morphological Changes ◽  
Complex Mixture ◽  
Ovarian Cancer Cells ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Apoptosis Pathway

Abstract The anti-tumor potential of animal toxins has fully attracted the attention of researchers. Snake venoms is a complex mixture of different components and has revealed high toxicity on normal and tumoral tissues or cells. The snake venom L-Amino-acid oxidase (svLAAO) has grown up to be a critical research target in molecular biology sciences and medicine sciences since widespread presence and various biological roles, including antitumor application. We found that Crotalus adamanteus (C. adamanteus) venom LAAO significantly decreased the viability of ovarian cancer cells and caused morphological changes preceded cell death. Cell experiments confirmed that C. adamanteus venom LAAO caused alterations of intrinsic or extrinsic apoptosis pathway-related genes in ovarian cancer cells. Animal experiments and histological analysis also proved that C. adamanteus venom LAAO could effectively inhibit the damage of ovarian cancer to tissues. The major apoptosis induction of C. adamanteus venom LAAO on ovarian cancer cells can be blocked by catalase, suggesting that the cytotoxicity of C. adamanteus venom LAAO on ovarian cancer cells was mainly mediated by H2O2. Our preliminary results revealed that C. adamanteus venom LAAO may induce apoptosis of ovarian cancer cells through the death receptor pathway and mitochondrial pathway. It is inferred that C. adamanteus venom LAAO will be some advantages in New Drug Research and Development of antitumor drugs in the future. Nevertheless, extra studies on the pharmacological actions and molecular mechanism of svLAAO in anti-cancer are necessary in order to better promote its application.

scholarly journals Size-Dependent Thermotherapy of Iron Oxide Nanoparticles on Human Breast Adenocarcinoma Cancer Cells

2013 ◽  
Vol 19 (S2) ◽  
pp. 218-219
Author(s):  
T. Mustafa ◽  
Y. Xu ◽  
F. Watanabe ◽  
Y. Zhang ◽  
M. Asar ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Breast Adenocarcinoma ◽  
Oxide Nanoparticles ◽  
Human Breast ◽  
Size Dependent ◽  
Human Breast Adenocarcinoma

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.

scholarly journals Dendrimer-Functionalized Iron Oxide Nanoparticles for Specific Targeting and Imaging of Cancer Cells

2007 ◽  
Vol 17 (16) ◽  
pp. 3043-3050 ◽  
Author(s):  
S. H. Wang ◽  
X. Shi ◽  
M. Van Antwerp ◽  
Z. Cao ◽  
S. D. Swanson ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Specific Targeting

Preparation of epidermal growth factor (EGF) conjugated iron oxide nanoparticles and their internalization into colon cancer cells

2010 ◽  
Vol 322 (15) ◽  
pp. 2244-2250 ◽  
Author(s):  
Mar Creixell ◽  
Adriana P. Herrera ◽  
Vanessa Ayala ◽  
Magda Latorre-Esteves ◽  
Marianela Pérez-Torres ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Iron Oxide ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cancer Cells ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Colon Cancer Cells ◽  
Epidermal Growth

Ferritin up-regulation and transient ROS production in cultured brain astrocytes after loading with iron oxide nanoparticles

2012 ◽  
Vol 8 (10) ◽  
pp. 3832-3839 ◽  
Author(s):  
Mark Geppert ◽  
Michaela C. Hohnholt ◽  
Sylvia Nürnberger ◽  
Ralf Dringen
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Ros Production
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