Biocompatible titanate nanotubes with high loading capacity of genistein: cytotoxicity study and anti-migratory effect on U87-MG cancer cell lines

RSC Advances ◽  
2016 ◽  
Vol 6 (103) ◽  
pp. 101688-101696 ◽  
Author(s):  
Tarek Baati ◽  
Bochra Bejaoui Kefi ◽  
Aicha Aouane ◽  
Leila Njim ◽  
Florence Chaspoul ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cellular Uptake ◽  
Direct Consequence ◽  
Titanate Nanotubes ◽  
Loading Capacity ◽  
Potential Candidate ◽  
Surface Change ◽  
Tubular Shape

Titanate nanotubes (Ti-Nts) have proved to be a potential candidate for drug delivery due to their large surface change and higher cellular uptake as a direct consequence of their tubular shape.

scholarly journals Super Magnetic Niosomal Nanocarrier as a New Approach for Treatment of Breast Cancer: A Case Study on SK-BR-3 and MDA-MB-231 Cell Lines

2021 ◽  
Vol 22 (15) ◽  
pp. 7948
Author(s):  
Elham Jamshidifar ◽  
Faten Eshrati Yeganeh ◽  
Mona Shayan ◽  
Mohammad Tavakkoli Yaraki ◽  
Mahsa Bourbour ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cellular Uptake ◽  
Targeted Delivery ◽  
Breast Cancer Cells ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Silica Layer

In the present study, a magnetic niosomal nanocarrier for co-delivery of curcumin and letrozole into breast cancer cells has been designed. The magnetic NiCoFe2O4 core was coated by a thin layer of silica, followed by a niosomal structure, allowing us to load letrozole and curcumin into the silica layer and niosomal layer, respectively, and investigate their synergic effects on breast cancer cells. Furthermore, the nanocarriers demonstrated a pH-dependent release due to the niosomal structure at their outer layer, which is a promising behavior for cancer treatment. Additionally, cellular assays revealed that the nanocarriers had low cellular uptake in the case of non-tumorigenic cells (i.e., MCF-10A) and related high viability but high cellular uptake in cancer cell lines (i.e., MDA-MB-231 and SK-BR-3) and related low viability, which is evidenced in their high cytotoxicity against different breast cancer cell lines. The cytotoxicity of the letrozole/curcumin co-loaded nanocarrier is higher than that of the aqueous solutions of both drugs, indicating their enhanced cellular uptake in their encapsulated states. In particular, NiCoFe2O4@L-Silica-L@C-Niosome showed the highest cytotoxicity effects on MDA-MB-231 and SK-BR-3 breast cancer cells. The observed cytotoxicity was due to regulation of the expression levels of the studied genes in breast cancer cells, where downregulation was observed for the Bcl-2, MMP 2, MMP 9, cyclin D, and cyclin E genes while upregulation of the expression of the Bax, caspase-3, and caspase-9 genes was observed. The flow cytometry results also revealed that NiCoFe2O4@L-Silica-L@C-Niosome enhanced the apoptosis rate in both MDA-MB-231 and SK-BR-3 cells compared to the control samples. The findings of our research show the potential of designing magnetic niosomal formulations for simultaneous targeted delivery of both hydrophobic and hydrophilic drugs into cancer cells in order to enhance their synergic chemotherapeutic effects. These results could open new avenues into the future of nanomedicine and the development of theranostic agents.

scholarly journals Ultra Flexible Nanocarrier for Enhanced the Ocular Delivery of Quercetin in Management of Macular Edema

2021 ◽  
Vol 3 (1) ◽  
pp. p9
Author(s):  
Sahu Seema ◽  
Shah Gulab Chand ◽  
Manigauha Ashish ◽  
Gupta Vandana
Keyword(s):  
Drug Delivery ◽  
Wound Healing ◽  
Macular Edema ◽  
Cell Lines ◽  
Cancer Cell ◽  
Drug Delivery Systems ◽  
Present Review ◽  
Plant Parts ◽  
Related Drug ◽  
Therapeutic Properties

Quercetin (Que) and its derivatives are naturally taking place phytochemicals with promising bioactive belongings. The antidiabetic, anti-inflammatory, antioxidant, antimicrobial, anti-Alzheimer’s, antiarthritic, cardiovascular, and wound-healing possessions of Que have been extensively investigated, as well as its anticancer commotion against different cancer cell lines has been newly reported. Que and its derivatives are found predominantly in the Western starve yourself, and people might benefit from their defensive effect just by taking them via diets or as a food enhancement. Bioavailability-related drug-delivery systems of Que have also been markedly exploited, and Que nanoparticles become visible as a promising proposal to enhance their bioavailability. The present review aims to make available a brief overview of the therapeutic things, new insights, and forthcoming perspectives of Que. Plants and plant parts are used for its aroma, flavor, or therapeutic properties. There are a number of recompense associated with using plants and plant phytoconstituents as contrasting to pharmaceutical merchandise.

scholarly journals CaCO3 as an Environmentally Friendly Renewable Material for Drug Delivery Systems: Uptake of HSA-CaCO3 Nanocrystals Conjugates in Cancer Cell Lines

Materials ◽  
2019 ◽  
Vol 12 (9) ◽  
pp. 1481 ◽  
Author(s):  
Viviana Vergaro ◽  
Isabella Pisano ◽  
Roberto Grisorio ◽  
Francesca Baldassarre ◽  
Rosanna Mallamaci ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cellular Uptake ◽  
Fluorescein Isothiocyanate ◽  
Protein Corona ◽  
Cancer Cell Lines ◽  
Human Model ◽  
Scanning Calorimetry ◽  
Alcohol Dehydration ◽  
Biological Responses

Chemical and biochemical functionalization of nanoparticles (NPs) can lead to an active cellular uptake enhancing their efficacy thanks to the targeted localization in tumors. In the present study calcium carbonate nano-crystals (CCNs), stabilized by an alcohol dehydration method, were successfully modified by grafting human serum albumin (HSA) on the surface to obtain a pure protein corona. Two types of CCNs were used: naked CaCO3 and the (3-aminopropyl)triethoxysilane (APTES) modified CaCO3-NH2. The HSA conjugation with naked CCN and amino-functionalized CCN (CCN-NH2) was established through the investigation of modification in size, zeta potential, and morphology by Transmission Electron Microscopy (TEM). The amount of HSA coating on the CCNs surface was assessed by spectrophotometry. Thermogravimetric analysis (TGA) and Differential scanning calorimetry (DSC) confirmed the grafting of APTES to the surface and successive adsorption of HSA. Furthermore, to evaluate the effect of protein complexation of CCNs on cellular behavior, bioavailability, and biological responses, three human model cancer cell lines, breast cancer (MCF7), cervical cancer (HeLa), and colon carcinoma (Caco-2) were selected to characterize the internalization kinetics, localization, and bio-interaction of the protein-enclosed CCNs. To monitor internalization of the various conjugates, chemical modification with fluorescein-isothiocyanate (FITC) was performed, and their stability over time was measured. Confocal microscopy was used to probe the uptake and confirm localization in the perinuclear region of the cancer cells. Flow cytometry assays confirmed that the bio-functionalization influence cellular uptake and the CCNs behavior depends on both cell line and surface features.

scholarly journals 3508 Ciclopirox Olamine Demonstrates Inhibitory Effects on Esophageal Tumor Cells

2019 ◽  
Vol 3 (s1) ◽  
pp. 5-5
Author(s):  
Randi Ryan ◽  
Shrikant Anant ◽  
Prabhu Ramamoorthy ◽  
Dharmalingam Subramaniam ◽  
Scott Weir
Keyword(s):  
Cell Cycle ◽  
Esophageal Cancer ◽  
Tumor Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Esophageal Tumor ◽  
Potential Candidate ◽  
Ciclopirox Olamine ◽  
Esophageal Cancer Cell

OBJECTIVES/SPECIFIC AIMS: Drug repositioning has the potential to accelerate translation of novel cancer chemotherapeutics from bench to bedside. The goal of this study was to determine the effects of ciclopirox olamine (CPX) on esophageal tumor cells. METHODS/STUDY POPULATION: We tested the effect of CPX on four esophageal cancer cell lines, assessing cell proliferation and viability by hexosaminidase and clonogenicity assay, respectively. We analyzed the effects of CPX on three-dimensional (3D) esophageal tumor cell spheroids. We also analyzed effects on cell cycle by flow cytometry. For mechanism, we performed western blots for proteins involved in cell cycle regulation, apoptosis and the Wnt/β-catenin pathway. For in vivo effects, we performed a murine xenograft model with intraperitoneal administration of CPX (100 mg/Kg body weight daily). RESULTS/ANTICIPATED RESULTS: CPX inhibited growth of all cell lines in a time and concentration-dependent manner. CPX also inhibited growth of esophageal spheroids. Cell cycle analysis demonstrated G0/G1 arrest in cells treated with CPX. Western blot analyses demonstrated decreased expression of cyclinD1, CDK4, CDK6, and transcriptionally active β-catenin, supporting the role of CPX in cell cycle inhibition and decreased β-catenin activity. Finally, treatment of nude mice with CPX significantly decreased tumor xenograft volume. DISCUSSION/SIGNIFICANCE OF IMPACT: CPX demonstrates anti-tumor properties in esophageal cancer cell lines. The current results justify further research into the mechanism of this inhibition. Additionally, given its established safety in humans, CPX is a potential candidate for repositioning as an adjunct treatment for esophageal cancer.

CYTOTOXICITY AND CELLULAR UPTAKE OF CELLULOSE NANOCRYSTALS

Nano LIFE ◽  
2012 ◽  
Vol 02 (03) ◽  
pp. 1241006 ◽  
Author(s):  
SHUPING DONG ◽  
ANJALI A. HIRANI ◽  
KATELYN R. COLACINO ◽  
YONG WOO LEE ◽  
MAREN ROMAN
Keyword(s):  
Drug Delivery ◽  
Cell Lines ◽  
Cellular Uptake ◽  
Exposure Time ◽  
Cellulose Nanocrystals ◽  
Drug Carriers ◽  
Raw 264.7 ◽  
Cytotoxic Effects ◽  
Microplate Reader ◽  
Ldh Assay

There is growing evidence that filamentous nanoparticles offer advantages over spherical ones in drug delivery applications. The purpose of this study was to assess the potential of rod-like, plant-derived cellulose nanocrystals (CNCs) for nanomedical uses. Besides a nonspherical morphology, their facile bioconjugation, surface hydrophilicity and small size render CNCs promising drug carriers. The cytotoxicity of CNCs against nine different cell lines (HBMEC, bEnd.3, RAW 264.7, MCF-10A, MDA-MB-231, MDA-MB-468, KB, PC-3 and C6) was determined by MTT and LDH assay. CNCs showed no cytotoxic effects against any of these cell lines in the concentration range and exposure time studied (0–50 μg/mL and 48 h, respectively). Cellular uptake of fluorescein-5′-isothiocyanate-labeled CNCs by these cell lines, quantified with a fluorescence microplate reader, was minimal. The lack of cytotoxicity and the low nonspecific cellular uptake support our hypothesis that CNCs are good candidates for nanomedical applications.

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