scholarly journals Sciatic nerve regeneration induced by glycosaminoglycan and laminin mimetic peptide nanofiber gels

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 110535-110547 ◽  
Author(s):  
Busra Mammadov ◽  
Melike Sever ◽  
Mevhibe Gecer ◽  
Fatih Zor ◽  
Sinan Ozturk ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Bioactive Peptide ◽  
Peripheral Nerve Injuries ◽  
Nerve Injuries ◽  
Mimetic Peptide ◽  
The Usa ◽  
Sciatic Nerve Regeneration

Bioactive peptide gels enhance the regeneration of peripheral nerve injuries, which affect 20 million patients in the USA.

scholarly journals Recovery of Peripheral Nerve with Massive Loss Defect by Tissue Engineered Guiding Regenerative Gel

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Shimon Rochkind ◽  
Zvi Nevo
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Nerve Injury ◽  
Peripheral Nerve Injury ◽  
Preclinical Study ◽  
Significant Loss ◽  
Peripheral Nerve Injuries ◽  
Nerve Injuries ◽  
Reconstructive Procedures ◽  
Nerve Conduits

Objective. Guiding Regeneration Gel (GRG) was developed in response to the clinical need of improving treatment for peripheral nerve injuries and helping patients regenerate massive regional losses in peripheral nerves. The efficacy of GRG based on tissue engineering technology for the treatment of complete peripheral nerve injury with significant loss defect was investigated.Background. Many severe peripheral nerve injuries can only be treated through surgical reconstructive procedures. Such procedures are challenging, since functional recovery is slow and can be unsatisfactory. One of the most promising solutions already in clinical practice is synthetic nerve conduits connecting the ends of damaged nerve supporting nerve regeneration. However, this solution still does not enable recovery of massive nerve loss defect.The proposed technologyis a biocompatible and biodegradable gel enhancing axonal growth and nerve regeneration. It is composed of a complex of substances comprising transparent, highly viscous gel resembling the extracellular matrix that is almost impermeable to liquids and gasses, flexible, elastic, malleable, and adaptable to various shapes and formats.Preclinical studyon rat model of peripheral nerve injury showed that GRG enhanced nerve regeneration when placed in nerve conduits, enabling recovery of massive nerve loss, previously unbridgeable, and enabled nerve regeneration at least as good as with autologous nerve graft “gold standard” treatment.

scholarly journals Stabilization, Rolling, and Addition of Other Extracellular Matrix Proteins to Collagen Hydrogels Improve Regeneration in Chitosan Guides for Long Peripheral Nerve Gaps in Rats

Neurosurgery ◽  
2017 ◽  
Vol 80 (3) ◽  
pp. 465-474 ◽  
Author(s):  
Francisco Gonzalez-Perez ◽  
Stefano Cobianchi ◽  
Claudia Heimann ◽  
James B. Phillips ◽  
Esther Udina ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Standard Technique ◽  
Biologically Active ◽  
Peripheral Nerve Injuries ◽  
Nerve Injuries ◽  
Critical Gap ◽  
The Right

Abstract BACKGROUND: Autograft is still the gold standard technique for the repair of long peripheral nerve injuries. The addition of biologically active scaffolds into the lumen of conduits to mimic the endoneurium of peripheral nerves may increase the final outcome of artificial nerve devices. Furthermore, the control of the orientation of the collagen fibers may provide some longitudinal guidance architecture providing a higher level of mesoscale tissue structure. OBJECTIVE: To evaluate the regenerative capabilities of chitosan conduits enriched with extracellular matrix-based scaffolds to bridge a critical gap of 15 mm in the rat sciatic nerve. METHODS: The right sciatic nerve of female Wistar Hannover rats was repaired with chitosan tubes functionalized with extracellular matrix-based scaffolds fully hydrated or stabilized and rolled to bridge a 15 mm nerve gap. Recovery was evaluated by means of electrophysiology and algesimetry tests and histological analysis 4 months after injury. RESULTS: Stabilized constructs enhanced the success of regeneration compared with fully hydrated scaffolds. Moreover, fibronectin-enriched scaffolds increased muscle reinnervation and number of myelinated fibers compared with laminin-enriched constructs. CONCLUSION: A mixed combination of collagen and fibronectin may be a promising internal filler for neural conduits for the repair of peripheral nerve injuries, and their stabilization may increase the quality of regeneration over long gaps.

The therapeutic potential of ex vivo expanded CD133+ cells derived from human peripheral blood for peripheral nerve injuries

2012 ◽  
Vol 117 (4) ◽  
pp. 787-794 ◽  
Author(s):  
Shin Ohtsubo ◽  
Masakazu Ishikawa ◽  
Naosuke Kamei ◽  
Yasumu Kijima ◽  
Osami Suzuki ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Peripheral Blood ◽  
Therapeutic Potential ◽  
Ex Vivo ◽  
Human Peripheral Blood ◽  
Peripheral Nerve Injuries ◽  
Nerve Injuries ◽  
Group 4 ◽  
Group 1

Object CD133+ cells have the potential to enhance histological and functional recovery from peripheral nerve injury. However, the number of CD133+ cells safely obtained from human peripheral blood is extremely limited. To address this issue, the authors expanded CD133+ cells derived from human peripheral blood using the serum-free expansion culture method and transplanted these ex vivo expanded cells into a model of sciatic nerve defect in rats. The purpose of this study was to determine the potential of ex vivo expanded CD133+ cells to induce or enhance the repair of injured peripheral nerves. Methods Phosphate-buffered saline (PBS group [Group 1]), 105 fresh CD133+ cells (fresh group [Group 2]), 105 ex vivo expanded CD133+ cells (expansion group [Group 3]), or 104 fresh CD133+ cells (low-dose group [Group 4]) embedded in atelocollagen gel were transplanted into a silicone tube that was then used to bridge a 15-mm defect in the sciatic nerve of athymic rats (10 animals per group). At 8 weeks postsurgery, histological and functional evaluations of the regenerated tissues were performed. Results After 1 week of expansion culture, the number of cells increased 9.6 ± 3.3–fold. Based on the fluorescence-activated cell sorting analysis, it was demonstrated that the initial freshly isolated CD133+ cell population contained 93.22% ± 0.30% CD133+ cells and further confirmed that the expanded cells had a purity of 59.02% ± 1.58% CD133+ cells. However, the histologically and functionally regenerated nerves bridging the defects were recognized in all rats in Groups 2 and 3 and in 6 of 10 rats in Group 4. The nerves did not regenerate to bridge the defect in any of the rats in Group 1. Conclusions The authors' results show that ex vivo expanded CD133+ cells derived from human peripheral blood have a therapeutic potential similar to fresh CD133+ cells for peripheral nerve injuries. The ex vivo procedure that can be used to expand CD133+ cells without reducing their function represents a novel method for developing cell therapy for nerve defects in a clinical setting.

Electroactive nanomaterials in the peripheral nerve regeneration

2021 ◽  
Author(s):  
Xiangyun Yao ◽  
Yun Qian ◽  
Cunyi Fan
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Regeneration ◽  
Life Quality ◽  
Peripheral Nerve Injuries ◽  
Human Beings ◽  
Nerve Injuries

Severe peripheral nerve injuries are threatening the life quality of human beings.

A PERIPHERAL NERVE INJURY MODEL UTILIZING A SIMPLE TISSUE SPARING APPROACH TO THE RABBIT SCIATIC NERVE

2018 ◽  
Vol 21 (01) ◽  
pp. 1850002
Author(s):  
Andrew J. Miller ◽  
Jacob Tulipan ◽  
Mark Wang ◽  
Pedro Beredjiklian ◽  
Andrzej Fertala ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Injury ◽  
Peripheral Nerve Injury ◽  
Rabbit Model ◽  
Peripheral Nerve Injuries ◽  
Nerve Injuries ◽  
Muscle Sparing ◽  
Tissue Sparing ◽  
Injury Models

Background & Aims: Peripheral nerve injury models require a reproducible surgical technique that provides a simple and extensile peripheral nerve exposure. We present a muscle sparing apprach to the sciatic nerve in a rabbit model. A combination of general anestheia, sedative and perioperative analgesics was given to minimize animal discomfort. Methods: A posterior lateral dissection of the rabbit hind leg was performed to expose the proximal sciatic nerve. This facilitated an extensile dissection of both the proximal and distal sciatic nerve. Results: The described dissection is atraumatic, bloodless, and yields minimal postoperative morbidity on the rabbit. All rabbits received standard postoperative care in compliance with all regulatory agencies. A full vetinarian staff was available to manage the animals postoperatively. Conclusion: We found that our technique is a reproducible and easy to perform surgical approach with basic surgical setup. Our animal model provides an opportunity to evaluate and study peripheral nerve injuries peripheral nerve injuries.

Use of electrospinning to construct biomaterials for peripheral nerve regeneration

2016 ◽  
Vol 27 (7) ◽  
pp. 761-768 ◽  
Author(s):  
Qi Quan ◽  
Biao Chang ◽  
Hao Ye Meng ◽  
Ruo Xi Liu ◽  
Yu Wang ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Functional Recovery ◽  
Nerve Repair ◽  
Peripheral Nerve Regeneration ◽  
Peripheral Nerve Injuries ◽  
Nerve Injuries ◽  
New Process ◽  
Recent Developments ◽  
Innovative Techniques

AbstractA number of limitations associated with the use of hollow nerve guidance conduits (NGCs) require further discussion. Most importantly, the functional recovery outcomes after the placement of hollow NGCs are poor even after the successful bridging of peripheral nerve injuries. However, nerve regeneration scaffolds built using electric spinning have several advantages that may improve functional recovery. Thus, the present study summarizes recent developments in this area, including the key cells that are combined with the scaffold and associated with nerve regeneration, the structure and configuration of the electrospinning design (which determines the performance of the electrospinning scaffold), the materials the electrospinning fibers are composed of, and the methods used to control the morphology of a single fiber. Additionally, this study also discusses the processes underlying peripheral nerve regeneration. The primary goals of the present review were to evaluate and consolidate the findings of studies that used scaffolding biomaterials built by electrospinning used for peripheral nerve regeneration support. It is amazing that the field of peripheral nerve regeneration continues to consistently produce such a wide variety of innovative techniques and novel types of equipment, because the introduction of every new process creates an opportunity for advances in materials for nerve repair.

Effects of topically administered FK506 on sciatic nerve regeneration and reinnervation after vein graft repair of short nerve gaps

2012 ◽  
Vol 32 (5) ◽  
pp. E5 ◽  
Author(s):  
Saeed Azizi ◽  
Rahim Mohammadi ◽  
Keyvan Amini ◽  
Roza Fallah
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Vein Graft ◽  
S100 Protein ◽  
Functional Study ◽  
Control Group ◽  
Significant Difference ◽  
Regenerated Fibers ◽  
Sciatic Nerve Regeneration

Object Despite the development of various nerve coaptation materials and techniques, achievement of desired functional peripheral nerve regeneration is still inadequate, and repair of peripheral nerve injuries is still one of the most challenging tasks and concerns in neurosurgery. The effect of an FK506-loaded vein graft as an in situ delivery system for FK506 in bridging the defects was studied using a rat sciatic nerve regeneration model. Methods A 10-mm sciatic nerve defect was bridged using an inside-out vein graft (IOVG) filled with 10 μl of a carrier-drug dilution (10 ng/ml FK506) in the IOVG/FK506 group. In the IOVG control group, the vein was filled with the same volume of carrier dilution alone. The regenerated fibers were studied 4, 8, and 12 weeks after surgery. Results Functional study confirmed faster recovery of the regenerated axons in the IOVG/FK506 group than in the IOVG group (p < 0.05). There was a statistically significant difference between the mean gastrocnemius muscle weight ratios of the IOVG/FK506 and IOVG control groups (p < 0.05). Morphometric indices of regenerated fibers showed that the number and diameter of the myelinated fibers were significantly higher in the IOVG/FK506 group than in the IOVG control group. Immunohistochemical analysis showed more positive immunoreactivity to S100 protein in the IOVG/FK506 group than in the IOVG control group. Conclusions When loaded in a vein graft, FK506 resulted in improvement of functional recovery and quantitative morphometric indices of sciatic nerve. Topical application of this readily available agent offers the benefit of cost savings as well as avoiding the complications associated with systemic administration.

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