scholarly journals SBA-15 mesoporous silica particles loaded with cisplatin induce senescence in B16F10 cells

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 111031-111040 ◽  
Author(s):  
David Edeler ◽  
Milena R. Kaluđerović ◽  
Biljana Dojčinović ◽  
Harry Schmidt ◽  
Goran N. Kaluđerović
Keyword(s):  
Mesoporous Silica ◽  
Melanoma Cells ◽  
Silica Particles ◽  
B16f10 Melanoma ◽  
B16f10 Cells ◽  
B16f10 Melanoma Cells

Nanoparticles obtained by loading of cisplatin into mesoporous silica SBA-15 (SBA-15|CP) change the phenotype of surviving B16F10 melanoma cells from malignant to senescent.

scholarly journals Melanogenesis Effect of 7-acetoxy-4-methylcoumarin in B16F10 Melanoma Cells

Cosmetics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 94
Author(s):  
Ji-Han Sim ◽  
Sung-Chan Jang ◽  
Tae-Jin Park ◽  
Won-Jae Chi ◽  
Seung-Young Kim
Keyword(s):  
Melanoma Cells ◽  
Hair Follicles ◽  
Dependent Manner ◽  
B16f10 Melanoma ◽  
Melanin Production ◽  
B16f10 Cells ◽  
B16f10 Melanoma Cells ◽  
Diphenyl Tetrazolium Bromide ◽  
Synthetic Derivatives ◽  
Dose Dependent

The increased interest in anti-whitening dyes has enhanced the research interest to identify efficient melanogenic activators. Melanogenesis is the process of melanin production by melanocytes in the hair follicles and skin, which is mediated by several enzymes, such as microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), tyrosinase-related protein (TRP)-1, and TRP-2. This study investigated the melanogenesis-stimulating effect of 4-Methylumbelliferone (4MUMB) and its synthetic derivatives, 7-acetoxy-4-methylcoumarin (7A4MC) and 4-methylheriniarin (4MH) in B16F10 melanoma cells. The cytotoxicity of these compounds was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, followed by the assessment of the melanin content and the intracellular TYR activity. Finally, the expression levels of the key enzymes involved in melanogenesis were investigated. 7A4MC increased melanin production in B16F10 cells relative to that by 4MUMB and 4MH treated cells in a dose-dependent manner without significant cytotoxicity. Concomitantly, 7A4MC significantly increased TYR activity and enhanced the expression of MITF, which significantly induced the expression of TRP-1, TRP-2, and TYR. Furthermore, 7A4MC stimulated melanogenesis via increased phosphorylation of c-Jun N-terminal kinases (JNK) and reduced phosphorylation of protein kinase B (AKT). These results confirmed the melanogenesis-inducing effects of 7A4MC and indicated its potential use as an anti-hair bleaching agent in cosmetics industries.

scholarly journals Tobramycin Promotes Melanogenesis by Upregulating p38 MAPK Protein Phosphorylation in B16F10 Melanoma Cells

Antibiotics ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 140 ◽  
Author(s):  
Seung-Hyun Moon ◽  
You Chul Chung ◽  
Chang-Gu Hyun
Keyword(s):  
Protein Phosphorylation ◽  
Melanoma Cells ◽  
Clinical Use ◽  
Melanin Content ◽  
B16f10 Melanoma ◽  
Melanin Production ◽  
B16f10 Cells ◽  
Infection Treatment ◽  
B16f10 Melanoma Cells ◽  
Long Time

Tobramycin is an aminoglycoside-based natural antibiotic derived from Streptomyces tenebrarius, which is primarily used for Gram-negative bacterial infection treatment. Although tobramycin has been utilized in clinical practice for a long time, it has exhibited several side effects, leading to the introduction of more effective antibiotics. Therefore, we conducted our experiments focusing on new possibilities for the clinical use of tobramycin. How tobramycin affects skin melanin formation is unknown. This study used B16F10 melanoma cells to assess the effect of tobramycin on melanin production. After cytotoxicity was assessed by MTT assay, melanin content and tyrosinase activity analyses revealed that tobramycin induces melanin synthesis in B16F10 cells. Next, Western blot analyses were performed to elucidate the mechanism by which tobramycin increases melanin production; phosphorylated p38 protein expression was upregulated. Protein inhibitors have been used to elucidate the mechanism of tobramycin. Kanamycin A and B are structurally similar to tobramycin, and 2-DOS represents the central structure of these antibiotics. The effects of these substances on melanogenesis were evaluated. Kanamycin A reduced melanin production, whereas kanamycin B and 2-DOS had no effect. Overall, our data indicated that tobramycin increases melanin production by promoting p38 protein phosphorylation in B16F10 melanoma cells.

Inhibitory Effects of Myelophycus simplex Papenfuss Methanol Extract on Melanogenesis in B16F10 Melanoma Cells

2017 ◽  
Vol 46 (1) ◽  
pp. 34-38
Author(s):  
Hyang Suk Kim ◽  
Ji Min Cheon ◽  
Da Hye Kwon ◽  
Eun Ok Choi ◽  
Min Ju Kim ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Melanoma Cells ◽  
Inhibitory Effects ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

Influence of host defenses on the hepatic colonization of B16F10 melanoma cells

1988 ◽  
Vol 6 (2) ◽  
pp. 153-169 ◽  
Author(s):  
E. Barberá-Guillem ◽  
M. L. Cañavate ◽  
I. Lopez De Tejada ◽  
F. Vidal-Vanaclocha
Keyword(s):  
Melanoma Cells ◽  
Host Defenses ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells

Inhibition of melanogenesis by Erigeron canadensis via down-regulating melanogenic enzymes in B16F10 melanoma cells

2008 ◽  
Vol 25 (6) ◽  
pp. 1463-1466 ◽  
Author(s):  
Eun-Suk Hong ◽  
Duc Thi Minh Nguyen ◽  
Dung Hoang Nguyen ◽  
Eun-Ki Kim
Keyword(s):  
Melanoma Cells ◽  
B16f10 Melanoma ◽  
Erigeron Canadensis ◽  
B16f10 Melanoma Cells

Antimelanogenic Effects ofRaphanus sativusL. var.nigerRoots on α-MSH Stimulated B16F10 Melanoma Cells

2018 ◽  
Vol 39 (11) ◽  
pp. 1254-1258
Author(s):  
Ha Na Ko ◽  
Jung Eun Kim ◽  
Yeon Jeong Jo ◽  
Seung Hyun Hong ◽  
Da Wun Yang ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
B16f10 Melanoma ◽  
B16f10 Melanoma Cells
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