A label-free kissing complex-induced fluorescence sensor for DNA and RNA detection by using DNA-templated silver nanoclusters as a signal transducer

RSC Advances ◽  
2016 ◽  
Vol 6 (101) ◽  
pp. 99269-99273 ◽  
Author(s):  
Kai Zhang ◽  
Ke Wang ◽  
Xue Zhu ◽  
Minhao Xie ◽  
Fei Xu
Keyword(s):  
Fluorescence Sensor ◽  
Silver Nanoclusters ◽  
Signal Transducer ◽  
Label Free ◽  
Rna Detection ◽  
Dna And Rna ◽  
New Strategy ◽  
Kissing Complex

A new strategy integrate silver nanoclusters (AgNCs) and riboswitches for the expanding of the application of a kissing complexes-induced sensor (KCIS) for the assay of DNA and RNA was reported.

scholarly journals Fast Detection of Chloramphenicol in Raw Milk Using a Hairpin Aptamer-templated Silver Nanoclusters

2021 ◽  
pp. 8-21
Author(s):  
Hengchao Li ◽  
Bin Hu ◽  
Yuliang Cheng ◽  
Hang Yu ◽  
Yunfei Xie ◽  
...  
Keyword(s):  
Detection Limit ◽  
Raw Milk ◽  
Hairpin Structure ◽  
Silver Nanoclusters ◽  
Structure Transformation ◽  
Label Free ◽  
Fast Detection ◽  
New Strategy ◽  
Potential Applicability ◽  
Induced Structure

In this presented work, a facile and efficient method was established for the detection of chloramphenicol (CAP) based on target-induced structure transformation of aptamer. This aptamer DNA with a hairpin structure can coincidentally serve as a template for the synthesis of bright silver nanoclusters (quantum yield 16.36%). The binding of CAP with aptamer DNA could cause the destruction of the hairpin structure, resulting in the quenching of the fluorescence of silver nanoclusters (AgNCs). It costs less than 10 minutes to complete the assay, and excellent sensitivity was achieved with detection limit of 0.052 nmol/L. The selectivity and recovery experiments also demonstrated satisfactory results of this proposed protocol. The method has potential applicability, and provides a new strategy for the development of label-free sensors based on aptamer and AgNCs.

scholarly journals G-quadruplexes: a promising target for cancer therapy

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Nils Kosiol ◽  
Stefan Juranek ◽  
Peter Brossart ◽  
Annkristin Heine ◽  
Katrin Paeschke
Keyword(s):  
Breast Cancer ◽  
Structure Formation ◽  
Genome Instability ◽  
Treatment Options ◽  
Personalized Treatment ◽  
Cancer Development ◽  
Intratumor Heterogeneity ◽  
Dna And Rna ◽  
New Strategy ◽  
The Stability

AbstractDNA and RNA can fold into a variety of alternative conformations. In recent years, a particular nucleic acid structure was discussed to play a role in malignant transformation and cancer development. This structure is called a G-quadruplex (G4). G4 structure formation can drive genome instability by creating mutations, deletions and stimulating recombination events. The importance of G4 structures in the characterization of malignant cells was currently demonstrated in breast cancer samples. In this analysis a correlation between G4 structure formation and an increased intratumor heterogeneity was identified. This suggests that G4 structures might allow breast cancer stratification and supports the identification of new personalized treatment options. Because of the stability of G4 structures and their presence within most human oncogenic promoters and at telomeres, G4 structures are currently tested as a therapeutic target to downregulate transcription or to block telomere elongation in cancer cells. To date, different chemical molecules (G4 ligands) have been developed that aim to target G4 structures. In this review we discuss and compare G4 function and relevance for therapeutic approaches and their impact on cancer development for three cancer entities, which differ significantly in their amount and type of mutations: pancreatic cancer, leukemia and malignant melanoma. G4 structures might present a promising new strategy to individually target tumor cells and could support personalized treatment approaches in the future.

Silver nanocluster-lightened hybridization chain reaction

RSC Advances ◽  
2016 ◽  
Vol 6 (62) ◽  
pp. 57502-57506 ◽  
Author(s):  
Lin Liu ◽  
Qing Li ◽  
Li-Juan Tang ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Nucleic Acid ◽  
Silver Nanoclusters ◽  
Hybridization Chain Reaction ◽  
Label Free ◽  
Chain Reaction ◽  
Silver Nanocluster ◽  
Turn On ◽  
Nucleic Acid Assays

A hybridization chain reaction (HCR) lightened by DNA-stabilized silver nanoclusters (AgNCs) as a label-free and turn on fluorescence platform for nucleic acid assays.

Label-free DNA detection based on oligonucleotide-stabilized silver nanoclusters and exonuclease III-catalyzed target recycling amplification

2014 ◽  
Vol 6 (15) ◽  
pp. 6082-6087 ◽  
Author(s):  
Hui Ma ◽  
Wei Wei ◽  
Qian Lu ◽  
Zhixin Zhou ◽  
Henan Li ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Silver Nanoclusters ◽  
Label Free ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Free Dna ◽  
Sensitivity And Selectivity ◽  
Exo Iii ◽  
Ag Ncs ◽  
Recycling Amplification

A label-free DNA biosensor with high sensitivity and selectivity is constructed by using DNA–Ag NCs and Exo III-catalyzed target recycling amplification.

scholarly journals DNA/RNA Detection Using DNA-Templated Few-Atom Silver Nanoclusters

Biosensors ◽  
2013 ◽  
Vol 3 (2) ◽  
pp. 185-200 ◽  
Author(s):  
Judy Obliosca ◽  
Cong Liu ◽  
Robert Batson ◽  
Mark Babin ◽  
James Werner ◽  
...  
Keyword(s):  
Silver Nanoclusters ◽  
Rna Detection

A Fluorescence Sensor for Lead (II) Ions Determination Based on Label-Free Gold Nanoparticles (GNPs)-DNAzyme Using Time-Gated Mode in Aqueous Solution

2016 ◽  
Vol 27 (2) ◽  
pp. 643-649 ◽  
Author(s):  
Xiao-Yu Wang ◽  
Cheng-Gang Niu ◽  
Li-Juan Guo ◽  
Liu-Yin Hu ◽  
Sheng-Quan Wu ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Gold Nanoparticles ◽  
Fluorescence Sensor ◽  
Label Free ◽  
Free Gold

scholarly journals A Combined ELONA-(RT)qPCR Approach for Characterizing DNA and RNA Aptamers Selected against PCBP-2

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1213 ◽  
Author(s):  
Miguel Moreno ◽  
María Fernández-Algar ◽  
Javier Fernández-Chamorro ◽  
Jorge Ramajo ◽  
Encarnación Martínez-Salas ◽  
...  
Keyword(s):  
Binding Capacity ◽  
Rna Aptamers ◽  
Dna Aptamers ◽  
General Strategy ◽  
Label Free ◽  
Mobility Shift ◽  
Real Time Quantitative Pcr ◽  
High Affinity ◽  
Dna And Rna ◽  
Electrophoretic Mobility Shift Assays

Improvements in Systematic Evolution of Ligands by EXponential enrichment (SELEX) technology and DNA sequencing methods have led to the identification of a large number of active nucleic acid molecules after any aptamer selection experiment. As a result, the search for the fittest aptamers has become a laborious and time-consuming task. Herein, we present an optimized approach for the label-free characterization of DNA and RNA aptamers in parallel. The developed method consists in an Enzyme-Linked OligoNucleotide Assay (ELONA) coupled to either real-time quantitative PCR (qPCR, for DNA aptamers) or reverse transcription qPCR (RTqPCR, for RNA aptamers), which allows the detection of aptamer-target interactions in the high femtomolar range. We have applied this methodology to the affinity analysis of DNA and RNA aptamers selected against the poly(C)-binding protein 2 (PCBP-2). In addition, we have used ELONA-(RT)qPCR to quantify the dissociation constant (Kd) and maximum binding capacity (Bmax) of 16 high affinity DNA and RNA aptamers. The Kd values of the high affinity DNA aptamers were compared to those derived from colorimetric ELONA performed in parallel. Additionally, Electrophoretic Mobility Shift Assays (EMSA) were used to confirm the binding of representative PCBP-2-specific RNA aptamers in solution. We propose this ELONA-(RT)qPCR approach as a general strategy for aptamer characterization, with a broad applicability in biotechnology and biomedicine.

scholarly journals Flavylium Dye as pH-Tunable Fluorescent and CD Probe for Double-Stranded DNA and RNA

Chemosensors ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 129
Author(s):  
Ivo Crnolatac ◽  
Letícia Giestas ◽  
Gordan Horvat ◽  
António Jorge Parola ◽  
Ivo Piantanida
Keyword(s):  
Steady State ◽  
Detection Limit ◽  
Rna Secondary Structure ◽  
Binding Affinities ◽  
Cationic Form ◽  
Rna Detection ◽  
Time Resolved ◽  
Dna And Rna ◽  
Double Stranded Dna ◽  
Acidic Conditions

The interaction of 4′-(N,N-dimethylamino)-6-hydroxyflavylium cation with double stranded (ds-) DNA/RNA was studied by UV/Vis spectrophotometry, circular dichroism (CD), and also steady-state and time-resolved emission spectroscopies at neutral and weakly acidic conditions. At pH 5, the studied molecule, in its flavylium cationic form, showed considerable binding affinities (5 < logKs < 6) for all ds-DNA/RNA, contrary to chalcones forms (dominant at pH 7), which did not show binding to polynucleotides. Flavylium cation intercalated into ds-DNAs at variance to dominant groove aggregation within ds-RNA, which was reported by RNA-specific bisignate induced CD spectrum (ICD) bands. The intrinsically negligible fluorescence of flavylium was strongly increased upon the addition of DNA or RNA, whereby both the fluorescence intensity and emission lifetimes of complexes differed considerably: the strongest emission increase was observed for AU-RNA (detection limit estimated to 10 nM) followed by AT-DNAs and the much weaker effect of GC-DNAs. Both fluorescence sensitivity on the ds-DNA/RNA secondary structure and sequence-selective ICD bands make the flavylium–chalcones system an intriguing pH-switchable new probe for distinguishing between various polynucleotide sequences.

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