Combined pretreatment of lignocellulosic biomass by solid base (calcined Na2SiO3) and ionic liquid for enhanced enzymatic saccharification

RSC Advances ◽  
2016 ◽  
Vol 6 (101) ◽  
pp. 99455-99466 ◽  
Author(s):  
Xiyan Sun ◽  
Xitong Sun ◽  
Fan Zhang

Combined pretreatment of lignocellulose by [BMIm]Cl and solid base Na2SiO3 enhances the enzymatic hydrolysis of willow and soybean straw.

RSC Advances ◽  
2018 ◽  
Vol 8 (19) ◽  
pp. 10207-10214 ◽  
Author(s):  
Wei Liu ◽  
Wei Chen ◽  
Qingxi Hou ◽  
Si Wang ◽  
Fang Liu

The combined pretreatment of dilute acid pre-extraction and mechanical refining significantly improved the enzymatic hydrolysis performance of lignocellulosic biomass.


2016 ◽  
Vol 109 ◽  
pp. 252-267 ◽  
Author(s):  
Amal A. Elgharbawy ◽  
Md Zahangir Alam ◽  
Muhammad Moniruzzaman ◽  
Masahiro Goto

2012 ◽  
Vol 43 (4) ◽  
pp. 573-577 ◽  
Author(s):  
Chia-Hung Su ◽  
Ming-Hua Chung ◽  
Hsin-Ju Hsieh ◽  
Yu-Kaung Chang ◽  
Jin-Chau Ding ◽  
...  

BioResources ◽  
2017 ◽  
Vol 12 (4) ◽  
pp. 7834-7840 ◽  
Author(s):  
Jungwoo Yang ◽  
Ji Eun Kim ◽  
Jae Kyun Kim ◽  
Sung ho Lee ◽  
Ju-Hyun Yu ◽  
...  

The performance of cellulase in the enzymatic saccharification of lignocellulose depends on the characteristics of lignocellulosic biomass feedstocks and the pretreatment method used. Efficient hydrolysis of specifically pretreated lignocellulose necessitates the knowledge of the characteristics of the optimal commercial cellulases. In this study, commercial cellulase preparations (Accellerase™ 1000, Accellerase® 1500, and Spezyme® CP from DuPont and Cellic® CTec2 from Novozymes) were evaluated for their hydrolysis efficiency of hydrothermally pretreated empty fruit bunches (EFBs). The highest glucose yields of 91.3% and 84.7% were achieved for 30 FPU of Cellic® CTec2/g glucan with and without Cellic® HTec2, respectively. Of the four cellulases tested, Cellic® CTec2, which showed the highest cellobiohydrolase, xylanase, and β-glucosidase activities, showed the highest glucose yield in the enzymatic hydrolysis of hydrothermally pretreated EFBs. The results of this study are valuable for those who plan to enzymatically hydrolyze hydrothermally pretreated EFBs.


2020 ◽  
Vol 54 (3) ◽  
pp. 599-613
Author(s):  
Song-Yi Han ◽  
Chan-Woo Park ◽  
Takashi Endo ◽  
Fauzi Febrianto ◽  
Nam-Hun Kim ◽  
...  

2014 ◽  
Vol 98 (12) ◽  
pp. 5765-5774 ◽  
Author(s):  
Yaping Shang ◽  
Rongxin Su ◽  
Renliang Huang ◽  
Yang Yang ◽  
Wei Qi ◽  
...  

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1360
Author(s):  
Ekaterina Budenkova ◽  
Stanislav Sukhikh ◽  
Svetlana Ivanova ◽  
Olga Babich ◽  
Vyacheslav Dolganyuk ◽  
...  

Enzymatic hydrolysis of cellulose-containing raw materials, using Aspergillus niger, were studied. Filter paper, secondary cellulose-containing or starch-containing raw materials, miscanthus cellulose after alkaline or acid pretreatment, and wood chip cellulose, were used as substrates. The study focused on a wild A. niger strain, treated, or not (control), by ultraviolet (UV) irradiations for 45, 60, or 120 min (UV45, UV60, or UV120), or by UV irradiation for 120 min followed by a chemical treatment with NaN3 + ItBr for 30 min or 80 min (UV120 + CH30 or UV120 + CH80). A mixture of all the A. niger strains (MIX) was also tested. A citrate buffer, at 50 mM, wasthe most suitable for enzymatic hydrolysis. As the UV exposure time increased to 2 h, the cellulase activity of the surviving culturewas increased (r = 0.706; p < 0.05). The enzymatic activities of the obtained strains, towards miscanthus cellulose, wood chips, and filter paper, were inferior to those obtained with commercial enzymes (8.6 versus 9.1 IU), in some cases. Under stationary hydrolysis at 37 °C, pH = 4.7, the enzymatic activity of A. niger UV120 + CH30 was 24.9 IU. The enzymatic hydrolysis of secondary raw materials, using treated A. niger strains, was themost effective at 37 °C. Similarly, the most effective treatment of miscanthus cellulose and wood chips occurred at 50 °C. The maximum conversion of cellulose to glucose was observed using miscanthus cellulose (with alkaline pretreatment), and the minimum conversion was observed when using wood chips. The greatest value of cellulase activity was evidenced in the starch-containing raw materials, indicating that A. niger can ferment not only through cellulase activity, but also via an amylolytic one.


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