The impact of zinc oxide nanoparticles on phosphorus removal and the microbial community in activated sludge in an SBR

RSC Advances ◽  
2016 ◽  
Vol 6 (99) ◽  
pp. 96706-96713 ◽  
Author(s):  
S. T. Wang ◽  
W. Q. Wang ◽  
Z. R. Zhang ◽  
H. You
Keyword(s):  
Zinc Oxide ◽  
Microbial Community ◽  
Activated Sludge ◽  
Phosphorus Removal ◽  
Zinc Oxide Nanoparticles ◽  
Cell Membranes ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
The Impact

10 and 20 mg L−1 ZnO NPs damaged the integrity of cell membranes and the microbial community and affected phosphorus removal.

The impact of zinc oxide nanoparticles on nitrification and the bacterial community in activated sludge in an SBR

RSC Advances ◽  
2015 ◽  
Vol 5 (82) ◽  
pp. 67335-67342 ◽  
Author(s):  
S. T. Wang ◽  
S. P. Li ◽  
W. Q. Wang ◽  
H. You
Keyword(s):  
Zinc Oxide ◽  
Bacterial Community ◽  
Activated Sludge ◽  
Zinc Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
The Impact

20 and 50 mg L−1 of ZnO NPs affect transformation of NH4+–N to NO2−–N and NO2−–N to NO3−–N.

scholarly journals The Impact of Zinc Oxide Nanoparticles on Male (In)Fertility

Materials ◽  
2020 ◽  
Vol 13 (4) ◽  
pp. 849 ◽  
Author(s):  
Ana Rita Pinho ◽  
Sandra Rebelo ◽  
Maria de Lourdes Pereira
Keyword(s):  
Zinc Oxide ◽  
Reproductive System ◽  
Male Fertility ◽  
Zinc Oxide Nanoparticles ◽  
Engineered Nanoparticles ◽  
Male Reproductive System ◽  
Oxide Nanoparticles ◽  
Exceptional Set ◽  
Zno Nps ◽  
The Impact

Zinc oxide nanoparticles (ZnO NPs) are among nanoscale materials, attracting increasing attention owing to their exceptional set of characteristics, which makes these engineered nanoparticles a great option for improving the quality and effectiveness of diagnosis and treatment. The capacity of ZnO NPs to induce reactive oxygen species (ROS) production, DNA damage, and apoptosis represents a promise for their use in both cancer therapy and microbial treatment. However, their intrinsic toxicity together with their easy entrance and accumulation in organism have raised some concerns regarding the biomedical use of these NPs. Several studies have reported that ZnO NPs might induce cytotoxic effects on the male reproductive system, compromising male fertility. Despite some advances in this area, the knowledge of the effects of ZnO NPs on male fertility is still scarce. Overall, a brief outline of the major ZnO NPs biomedical applications and promises in terms of diagnostic and therapeutic use will also be explored. Further, this review intends to discuss the effect of ZnO NPs exposure on the male reproductive system and speculate their effects on male (in)fertility.

scholarly journals Long-Term Impact of Zinc Oxide Nanoparticles on Differentiation and Cytokine Secretion of Human Adipose-Derived Stromal Cells

Materials ◽  
2019 ◽  
Vol 12 (11) ◽  
pp. 1823 ◽  
Author(s):  
Katrin Radeloff ◽  
Andreas Radeloff ◽  
Mario Ramos Tirado ◽  
Agmal Scherzad ◽  
Rudolf Hagen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Zinc Oxide ◽  
Stromal Cells ◽  
Zinc Oxide Nanoparticles ◽  
Caspase 3 ◽  
Cytokine Secretion ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
The Impact

Zinc oxide nanoparticles (ZnO-NPs) are widely utilized, for example in manufacturing paints and in the cosmetic industry. In addition, there is raising interest in the application of NPs in stem cell research. However, cytotoxic, genotoxic and pro-inflammatory effects were shown for NPs. The aim of this study was to evaluate the impact of ZnO-NPs on cytokine secretion and differentiation properties of human adipose tissue-derived stromal cells (ASCs). Human ASCs were exposed to the subtoxic concentration of 0.2 µg/mL ZnO-NPs for 24 h. After four weeks of cultivation, adipogenic and osteogenic differentiation procedures were performed. The multi-differentiation potential was confirmed histologically and using polymerase chain reaction (PCR). In addition, the gene expression of IL-6, IL-8, vascular endothelial growth factor (VEGF) and caspase 3 was analyzed. Over the course of four weeks after ZnO-NPs exposure, no significant differences were detected in the gene expression of IL-6, IL-8, VEGF and caspase 3 compared to non-exposed cells. The differentiation was also not affected by the ZnO-NPs. These findings underline the fact, that functionality of ASCs is likely to be unaffected by ZnO-NPs, despite a long-term disposition of NPs in the cells, supposing that the starting concentration was safely in the non-toxic range. This might provide important information for single-use nanomedical applications of ZnO-NPs.

scholarly journals Iron-doped zinc oxide nanoparticles-triggered elicitation of important phenolic compounds in cell cultures of Fagonia indica.

2021 ◽  
Author(s):  
Atta Ullah Khan ◽  
Tariq Khan ◽  
Mubarak Ali Khan ◽  
Akhtar Nadhman ◽  
Muhammad Aasim ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Secondary Metabolism ◽  
Zinc Oxide Nanoparticles ◽  
Biomass Accumulation ◽  
Callus Cultures ◽  
Oxide Nanoparticles ◽  
Stem Explants ◽  
Zno Nps ◽  
Iron Doped ◽  
The Impact

Abstract Fagonia indica is an important medicinal plant species used traditionally against a variety of diseases. In this study, we initiated callus cultures from healthy stem explants. We observed maximum callus induction frequency (88%) on MS media supplemented with Thidiazuron (1.0 mg/mL). We also examined the callus cultures to determine the impact of iron-doped zinc oxide nanoparticles (Fe-ZnO-NPs) in concentrations (15.62 to 250 µg/mL) on biomass accumulation, secondary metabolism, and antioxidative potential in callus cultures of F. indica. Our results showed that maximum callus biomass (FW = 13.6 g and DW = 0.58 ± 0.01) was produced on day 40 when the media was supplemented with 250 µg/mL Fe-ZnO-NPs. Similarly, maximum TPC (268.36 µg GAE/g of DW) was detected in 40 days old callus added with 125 µg/mL Fe-ZnO-NPs. Maximum TFC (78.56 µg QE/g of DW) was observed in 20 days old callus grown in 62.5 µg/mL Fe-ZnO-NPs containing media. Maximum total antioxidant capacity (390.74 µg AAE/g of DW) was observed in 40 days old callus with 125 µg/mL Fe-ZnO-NPs treated cultures, respectively. The antioxidant potential was in correlation with the TPC. These results showed that Fe-ZnO-NPs elicitors can increase the biomass and activate secondary metabolism in F. indica cells.

Biosynthesis of ZnO Nanostructures Using Azadirachta indica Leaf Extract and Their Effect on Seed Germination and Seedling Growth of Tomato: An Eco-Friendly Approach

2020 ◽  
Vol 15 (11) ◽  
pp. 1412-1422
Author(s):  
Nishat Arshi ◽  
Y. Prashanthi ◽  
Tentu Nageswara Rao ◽  
Faheem Ahmed ◽  
Shalendra Kumar ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Seed Germination ◽  
Azadirachta Indica ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
X Ray ◽  
The Impact

In this study, we report synthesis of Zinc oxide nanoparticles using simple chemical and green methods. The ZnO nanoparticles were synthesized using leaf extract of Azadirachta indica (neem) as reducing agent. The as obtained product was characterized by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Energy dispersive X-ray analysis (EDAX) and TEM techniques. XRD analysis confirms that ZnO nanoparticles were crystalline having hexagonal Wurtzite structure with (1 0 0), (0 0 2), (1 0 1), (1 0 2), (1 1 0) and (1 1 2) planes. SEM analyses show that the as synthesized ZnO NPs were in the form of agglomerates and no other impurity peak was found in the EDS. TEM analyses confirm that the size of the nanoparticle was approx. 50 nm. Here in, we investigate the effect of chemical and green synthesized zinc oxide nanoparticles on germination and growth of lycopersicum esculentus (tomato) using petri plate seed germination method in loamy sand soil. The impact of concentration of applied ZnO nanoparticles via green synthesis and chemical methods were analyzed. Results revealed that green synthesized Zinc oxide nanoparticles showed maximum growth of seedling as compared to chemically synthesized Zinc oxide nanoparticles, bulk ZnO and control. After 50 days of tomato growth analysis, it was recognized that ZnO NPs can be a good green synthetic fertilizer by increasing shoot length, wet weight, dry weight and yield over conventional control. Hence, green method is found to be more effective.

scholarly journals Zinc Oxide Nanoparticles Cytotoxicity and Release from Newly Formed PMMA–ZnO Nanocomposites Designed for Denture Bases

Nanomaterials ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 1318 ◽  
Author(s):  
Mariusz Cierech ◽  
Jacek Wojnarowicz ◽  
Adam Kolenda ◽  
Agata Krawczyk-Balska ◽  
Emilia Prochwicz ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zno Nanoparticles ◽  
Zinc Oxide Nanoparticles ◽  
Host Cells ◽  
Emission Spectrometry ◽  
Oxide Nanoparticles ◽  
Pure Pmma ◽  
Zno Nps ◽  
Cytotoxicity Studies ◽  
The Impact

The goal of the study was to investigate the level of zinc oxide nanoparticles (ZnO NPs) release from polymethyl methacrylate (PMMA)–ZnO nanocomposites (2.5%, 5%, and 7.5% w/w), as well as from the ZnO NPs layer produced on pure PMMA, and the impact of the achieved final ZnO NPs concentration on cytotoxicity, before the potential use as an alternative material for denture bases. The concentration of ZnO nanoparticles released to the aqueous solution of Zn2+ ions was assessed using optical emission spectrometry with inductively coupled plasma (ICP-OES). In the control group (pure PMMA), the released mean for ZnO was 0.074 mg/L and for individual nanocomposites at concentrations of 2.5%, 5%, and 7.5% was 2.281 mg/L, 2.143 mg/L, and 3.512 mg/L, respectively. The median for the ZnO NPs layer produced on PMMA was 4.878 mg/L. In addition, in vitro cytotoxicity of ZnO NPs against the human HeLa cell line was determined through the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye. The cytotoxicity studies demonstrate that ZnO nanoparticles in the concentrations up to 20 mg/L have no adverse effect on HeLa cells. When compared with the released and cytotoxic concentrations of ZnO NPs, it can be expected that ZnO released from dental prostheses to the oral cavity environment will have no cytotoxic effect on host cells.

scholarly journals In Vitro Cytotoxicity Effects of Zinc Oxide Nanoparticles on Spermatogonia Cells

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1081
Author(s):  
Ana Rita Pinho ◽  
Filipa Martins ◽  
M. Elisabete V. Costa ◽  
Ana M. R. Senos ◽  
Odete A. B. da Cruz e Silva ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
The Body ◽  
Oxide Nanoparticles ◽  
Dependent Manner ◽  
Zno Nps ◽  
Cytotoxic Effects ◽  
Spermatogonia Cells ◽  
The Impact

Zinc Oxide Nanoparticles (ZnO NPs) are a type of metal oxide nanoparticle with an extensive use in biomedicine. Several studies have focused on the biosafety of ZnO NPs, since their size and surface area favor entrance and accumulation in the body, which can induce toxic effects. In previous studies, ZnO NPs have been identified as a dose- and time-dependent cytotoxic inducer in testis and male germ cells. However, the consequences for the first cell stage of spermatogenesis, spermatogonia, have never been evaluated. Therefore, the aim of the present work is to evaluate in vitro the cytotoxic effects of ZnO NPs in spermatogonia cells, focusing on changes in cytoskeleton and nucleoskeleton. For that purpose, GC-1 cell line derived from mouse testes was selected as a model of spermatogenesis. These cells were treated with different doses of ZnO NPs for 6 h and 12 h. The impact of GC-1 cells exposure to ZnO NPs on cell viability, cell damage, and cytoskeleton and nucleoskeleton dynamics was assessed. Our results clearly indicate that higher concentrations of ZnO NPs have a cytotoxic effect in GC-1 cells, leading to an increase of intracellular Reactive Oxygen Species (ROS) levels, DNA damage, cytoskeleton and nucleoskeleton dynamics alterations, and consequently cell death. In conclusion, it is here reported for the first time that ZnO NPs induce cytotoxic effects, including changes in cytoskeleton and nucleoskeleton in mouse spermatogonia cells, which may compromise the progression of spermatogenesis in a time- and dose-dependent manner.

scholarly journals Influence of zinc oxide nanoparticles on anaerobic digestion of waste activated sludge and microbial communities

RSC Advances ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 5580-5589
Author(s):  
Shutao Wang ◽  
Lingbo Chen ◽  
Hao Yang ◽  
Zhisheng Liu
Keyword(s):  
Zinc Oxide ◽  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Microbial Communities ◽  
Zinc Oxide Nanoparticles ◽  
Waste Activated Sludge ◽  
Oxide Nanoparticles ◽  
Zno Nps

The influence of long-term exposure of zinc oxide nanoparticles (ZnO NPs) to waste activated sludge on anaerobic digestion and microbial communities was studied.

scholarly journals Zinc Oxide Nanoparticles Improve Testicular Steroidogenesis Machinery Dysfunction In Benzo [α] Pyrene Challenged Rats

2021 ◽  
Author(s):  
Niveen M. Daoud ◽  
S Aly Mohamed ◽  
Omaima H. Ezzo ◽  
Naglaa A. Ali
Keyword(s):  
Oxidative Stress ◽  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Male Reproduction ◽  
Male Rats ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Positive Effects ◽  
Defensive Role ◽  
The Impact

Abstract Although Zinc oxide nanoparticles (ZnO NPs) in low doses have potentially positive effects on reproduction by their antioxidant effects, the defensive role of Zinc nanomaterials against environmental pollutants that affect male reproduction has not been adequately studied. We designed our study to assess the impact of ZnO NPs towards reproductive dysfunction induced by Benzo[α]Pyrene (B[a]P). Forty-eight mature male rats were randomly distributed into six equal groups: G1; negative control, G2&3- positive control I &II (either 10 or 30 mg ZnO NPs / kg BW); G4. (150 mg Bap / kg BW), G 5 & 6 (Co- administrated B[a]P with different concentrations of ZnO NPs). Oxidative stress biomarkers, semiquantitative real-time PCR for steroidogenic enzymes (CY11A1, StAR, and 3β- HSD), testosterone levels and histopathology in the liver, kidney, and testicles were examined for this investigation. B[a] P treated group showed significant deterioration in all reproductive parameters and induced oxidative stress. Co-administration ZnO NPs eased oxidative stress and effectively increased the expression of CY11A1, StAR, and 3β- HSD and improved histopathological changes in the examined organs. Our results using the selected doses and with Nano particle properties confirm that ZnO NPs have an obvious ameliorative effect against B[a] P.

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