NMR based metabolomic approach revealed cyclophosphamide-induced systematic alterations in a rat model

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 111020-111030 ◽  
Author(s):  
Tingli Qu ◽  
Erbing Wang ◽  
Aiping Li ◽  
GuanHua Du ◽  
Zhenyu Li ◽  
...  
Keyword(s):  
Rat Model ◽  
Biochemical Assay ◽  
H Nmr ◽  
Metabolomic Approach

A 1H NMR based metabolomics approach combined with biochemical assay had been employed to study the toxicity of CY.

scholarly journals Metabolomics reveal the protective effect of Farfarae Flos against asthma using an OVA-induced rat model

RSC Advances ◽  
2017 ◽  
Vol 7 (63) ◽  
pp. 39929-39939 ◽  
Author(s):  
Jing Li ◽  
Wei Gao ◽  
Jining Gao ◽  
Hong Li ◽  
Xiang Zhang ◽  
...  
Keyword(s):  
Protective Effect ◽  
Rat Model ◽  
Biochemical Assay ◽  
H Nmr

A 1H NMR based metabolomics approach combined with biochemical assay and histopathological inspection has been employed to study the protective effect of PEFF against asthma on a rat model.

scholarly journals Effect of Xueniao Capsule on Escherichia coli-Induced Acute Pyelonephritis Rats by 1H NMR-Based Metabolomic Approach

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Xuliang Hao ◽  
Pan He ◽  
Yan Ni ◽  
Jing Yao ◽  
Yankun Yang ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Acute Pyelonephritis ◽  
Bacterial Count ◽  
Action Mechanism ◽  
Chemical Difference ◽  
Future Studies ◽  
H Nmr ◽  
Multiple Components ◽  
Endogenous Metabolites ◽  
Metabolomic Approach

Xueniao capsule, one of the famous traditional Chinese medicine (TCM) formulas, has been proved to be effective for treating acute pyelonephritis (APN) in the clinic. However, the probable mechanisms are still unclear. This study was aimed at investigating the therapeutic effect and action mechanism of Xueniao capsule on acute pyelonephritis rats. Chemical analysis of Xueniao capsule and four different extracts was conducted by HPLC and GC-MS. 21 compounds were identified in the Xueniao capsule, and obvious chemical difference was also revealed among the different extracts by chemical analysis. Metabolomics, combined with bacteriological examination, traditional histopathology, and biochemical parameters, was used to evaluate the effects of Xueniao capsule and four different extracts. After treatment with Xueniao capsule, the bacterial count of urine was decreased and the renal lesions of APN rats were ameliorated by histopathology inspection. Levels of Scr and Ucr, IL-1α, IL-1β, IL-6, IL-10, CXCL-2, and MCP-1 were decreased significantly, and the reserving effect of Xueniao capsule was superior to the different extracts and norfloxacin. 16 endogenous metabolites related to APN model were revealed, and 12 of them could be reversed by the Xueniao capsule. 1H NMR metabolomic results demonstrated that the formula of Xueniao capsule played the best therapeutic role on APN through regulating energy metabolism and alterations of osmotic pressure. The effect of Xueniao capsule on the APN was the synergistic actions of multiple components, which need to be further investigated in future studies.

scholarly journals Infant urinary metabolomic profile in a fatal acute methadone intoxication

2022 ◽  
Author(s):  
Alberto Chighine ◽  
Michele Porcu ◽  
Giulio Ferino ◽  
Nicola Lenigno ◽  
Claudia Trignano ◽  
...  
Keyword(s):  
Perinatal Asphyxia ◽  
Sudden Infant Death ◽  
Sudden Infant ◽  
Metabolomic Profile ◽  
Metabolic Derangement ◽  
Additional Information ◽  
H Nmr ◽  
Healthy Control ◽  
Urinary Sample ◽  
Metabolomic Approach

AbstractA case report suspicious for a Sudden Infant Death Syndrome is here described. Pathological findings were consistent with an acute respiratory failure while toxicological analysis revealed an elevated blood methadone concentration. Death was then ascribed to an acute methadone intoxication. In addition to the routinary approach, the urinary sample collected at autopsy was investigated with a 1H NMR metabolomic approach and the identified metabolomic profile was challenged with the urinary metabolomic profiles previously obtained from 10 newborns who experienced perinatal asphyxia and 16 healthy control newborns. Intriguingly, the urinary profile of the methadone intoxicated infant was very similar to those belonging to the perinatal asphyxia newborns, especially to those belonging to the newborns characterised by the worst outcome. The results offer several hints on a shared metabolic derangement between different mechanisms of asphyxia/hypoxia. To the best of the authors’ knowledge, this is the first report of the use of a metabolomic approach in a pathological case, in which metabolomics offers useful additional information regarding the mechanism and the cause of death.

scholarly journals Monitoring the Modifications of the Vitreous Humor Metabolite Profile after Death: An Animal Model

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Maria Francesca Rosa ◽  
Paola Scano ◽  
Antonio Noto ◽  
Matteo Nioi ◽  
Roberta Sanna ◽  
...  
Keyword(s):  
Principal Component ◽  
Metabolite Profile ◽  
Vitreous Humor ◽  
Multivariate Statistical ◽  
H Nmr ◽  
Multivariate Statistical Approach ◽  
Compositional Changes ◽  
Latent Structures ◽  
Metabolomic Approach

We applied a metabolomic approach to monitor the modifications occurring in goat vitreous humor (VH) metabolite composition at different times (0, 6, 12, 18, and 24 hours) after death. The1H-NMR analysis of the VH samples was performed for the simultaneous determination of several metabolites (i.e., the metabolite profile) representative of the VHstatusat different times. Spectral data were analyzed by Principal Component Analysis (PCA) and by Orthogonal Projection to Latent Structures (OPLS) regression technique. PCA and OPLS suggested that different spectral regions were involved in time-related changes. The major time-related compositional changes, here detected, were the increase of lactate, hypoxanthine, alanine, total glutathione, choline/phosphocholine, creatine, andmyo-inositol and the decrease of glucose and 3-hydroxybutyrate. We attempted a speculative interpretation of the biological mechanisms underlying these changes. These results show that multivariate statistical approach, based on1H NMR metabolite profiling, is a powerful tool for detecting ongoing differences in VH composition and may be applied to investigate several physiological and pathological conditions.

scholarly journals Synthesis and Anti-Inflammatory Activity of a Novel Series of Diphenyl-1,2,4-triazoles and Related Derivatives

2012 ◽  
Vol 9 (4) ◽  
pp. 2510-2515 ◽  
Author(s):  
Swapna Cherala ◽  
Harikiran Lingabathula ◽  
Rama Ganta ◽  
Srinivas Ampati ◽  
Sarangapani Manda
Keyword(s):  
Spectral Analysis ◽  
Rat Model ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Mass Spectral Analysis ◽  
Thiourea Derivatives ◽  
Mass Spectral ◽  
H Nmr ◽  
Anti Inflammatory

In the present investigation we have synthesized a series of new 1-[3-(4-substitutedphenyl)-5-phenyl-4H-1,2,4-triazol-4-yl]urea and 1-[3-(4-substitutedphenyl)-5-phenyl-4H-1,2,4-triazol-4-yl]thiourea derivatives (4Ia - 4IId). The newly synthesised derivatives were characterized by using the data of IR,1H NMR and Mass Spectral analysis. Thus synthesised and characterized targetted compounds were further screened for their anti-inflammatory activity by using Carrageenan – induced paw edema rat model. Among all the newly synthesized derivatives, Compounds 4Ia-4Ic and Compounds 4IIa-4IId were reduced the inflammation very significantly (p<0.0001), thus these compounds showed promising anti-inflammatory activity and only one compound (4Id) showed moderate anti-inflammatory activity (p<0.05).

Soy protein modification of rat polycystic kidney disease

1998 ◽  
Vol 274 (3) ◽  
pp. F541-F549 ◽  
Author(s):  
Malcolm R. Ogborn ◽  
Neda Bankovic-Calic ◽  
Christen Shoesmith ◽  
Richard Buist ◽  
James Peeling
Keyword(s):  
Kidney Disease ◽  
Polycystic Kidney Disease ◽  
Rat Model ◽  
Soy Protein ◽  
Proton Nuclear Magnetic Resonance ◽  
Renal Tubular Cell ◽  
Ammonium Excretion ◽  
Polycystic Kidney ◽  
Nmr Studies ◽  
H Nmr

We undertook a study to determine whether soy protein feeding would ameliorate renal injury in the Han:SPRD- cy rat model of polycystic kidney disease (PKD). Male offspring of Han:SPRD- cy heterozygotes received isocaloric diets based on 20% casein or 20% heat-treated soy protein at weaning ad libitum for 8 wk. Soy-fed animals demonstrated lower serum creatinine (66 vs. 125 μmol/l; P = 0.002), lower urinary ammonium excretion (0.080 vs. 0.173 mmol/kg; P= 0.01), reduced renal cysts (0.98 vs. 4.92 ml/kg body wt, P < 0.0001), renal fibrosis (0.79 vs. 1.4 ml/kg; P = 0.016), macrophage infiltration, renal tubular cell proliferation, and apoptosis. Proton nuclear magnetic resonance (1H-NMR) studies of urine demonstrated that soy diet was associated with increased losses of citric acid cycle organic anions.1H-NMR of perchloric acid-extracted tissue found that levels of succinate were not depleted in soy-fed animals, despite increased urinary losses. Soy-fed animals had marked elevation of tissue betaine ( P < 0.001), with reduced taurine and cholines, compared with casein-fed animals ( P < 0.001). Soy feeding dramatically reduces both tubular and interstitial pathology in the Han:SPRD- cy rat model of PKD, through mechanisms that remain to be determined.

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