The impact of doped silicon quantum dots on human osteoblasts

RSC Advances ◽  
2016 ◽  
Vol 6 (68) ◽  
pp. 63403-63413 ◽  
Author(s):  
Lucie Ostrovska ◽  
Antonin Broz ◽  
Anna Fucikova ◽  
Tereza Belinova ◽  
Hiroshi Sugimoto ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Particle Size ◽  
Fetal Bovine Serum ◽  
Human Osteoblasts ◽  
Doped Silicon ◽  
Fetal Bovine ◽  
Biological Environment ◽  
The Impact ◽  
Si Quantum Dots ◽  
Co Doped

Interaction of red/infrared fluorescent (B, P co-doped) Si quantum dots with biological environment (medium and cells) is sensitive to the presence of fetal bovine serum, to the particle size and surface potential and influenced by particle ageing.

Effect of Cu on the Tribocorrosion Behavior of Antibacterial CoCrMo Alloys in Simulated Biological Environment

2020 ◽  
Vol 143 (7) ◽  
Author(s):  
Xuefeng Song ◽  
Ying Liu ◽  
Xiaoyan Wang ◽  
Erlin Zhang
Keyword(s):  
Antibacterial Property ◽  
Fetal Bovine Serum ◽  
Corrosion And Wear ◽  
Minimum Essential Medium ◽  
Cocrmo Alloy ◽  
Metal Implants ◽  
Cu Alloy ◽  
Fetal Bovine ◽  
Biological Environment ◽  
Cocrmo Alloys

Abstract CoCrMo alloy containing copper element can significantly improve the antibacterial property of the alloy. However, most metal implants often fail due to the presence of corrosion and wear. In this work, the electrochemical and tribocorrosion properties of CoCrMo, CoCrMo–2Cu, and CoCrMo–4Cu alloys at a potential of −0.45 V and 0 V condition in Hank’s/Saliva/α-minimum essential medium (MEM) containing 10% FBS solutions were studied to reveal the effect of Cu addition on the tribocorrosion properties of Co-based alloys. The results showed that the addition of copper element reduced the corrosion resistance and wear resistance of Co-based alloy. As for solutions, α-MEM containing 10% fetal bovine serum shows lubrication function. Based on the calculated results, it was pointed out that the tribocorrosion behavior of CoCrMo and CoCrMo–Cu alloy was mainly controlled by the mechanical wear.

Investigating the Impact of Serum on the Growth of TM7x and Host XH001

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Alice Khomski ◽  
Megan Pugach-Gordon
Keyword(s):  
Periodontal Disease ◽  
Culture Medium ◽  
Considerable Increase ◽  
Gingival Crevicular Fluid ◽  
Fetal Bovine Serum ◽  
Colony Forming Units ◽  
Fetal Bovine ◽  
Crevicular Fluid ◽  
Potential Pathogenicity ◽  
The Impact

Nanosynbacter lyticus strain TM7x is an ultra-small, parasitic bacterium that lives on the surface of host, Actinomyces odontolyticus strain XH001. Studies have reported a considerable increase in TM7x abundance in patients with periodontal disease, and this association warrants further investigation. Interestingly, gingival crevicular fluid (GCF) flow also increases during periodontitis. The prevalence of TM7x during periodontal disease may thus result from attraction to GCF. However, GCF is not commercially available for testing. Since its composition is similar to that of fetal bovine serum (FBS), FBS was tested instead. The objectives of this project are to determine whether FBS promotes the growth of TM7x and XH001 and to develop the optimal culture medium for analysis of these microbes. TM7x/XH001 co-culture and XH001 monoculture were separately cultivated in media (RPMI and FMC) supplemented with FBS. Cultures grown in the same, non-supplemented media served as control samples. Serial dilutions were performed and spotted onto plates at several time points, and samples were quantified in colony-forming units (CFU)/mL. 2% FBS FMC induced a significant increase in the growth of culture samples, as compared to control FMC (p = 0.0140 and p = 0.0424 for the co-culture and monoculture, respectively). Furthermore, microscopy revealed a heightened presence of TM7x in FBS-supplemented media in comparison to control media. These findings signify that FBS is an effective supplement to RPMI and FMC, both of which support TM7x/XH001 growth. Overall, the utilization of these media with FBS can ensure optimal cultivation of TM7x for investigation of its potential pathogenicity.

76 Tetrahydrofuran does not have Embryo Toxic Effects on In Vitro-Produced Bovine Embryos

2018 ◽  
Vol 30 (1) ◽  
pp. 176
Author(s):  
M. M. R. Chowdhury ◽  
I. Khan ◽  
A. Mesalam ◽  
K.-L. Lee ◽  
J.-Y. Hwang ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Bovine Serum ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Control Group ◽  
Bovine Embryos ◽  
Sodium Pyruvate ◽  
Fetal Bovine ◽  
The Impact

In vitro embryo developmental potentials are still suboptimal compared with in vivo potential due to the challenge of various unknown stressors that must be overcome by in vitro-cultured oocytes. To improve existing embryo developmental potentials, many chemicals have been treated in maturation media by dissolving in toxic substances such as dimethyl sulfoxide (DMSO) or other carrier molecule. The foremost effort of this study was to investigate the impact of the solvent tetrahydrofuran (THF) on the cytotoxicity of in vitro embryo production (IVP). The experiment was completed within 8 replicates. Statistical analyses were performed using SPSS version 22.0 (IBM/SPSS, Armonk, NY, USA), a one-way ANOVA followed by multiple pairwise comparisons (Tukey’s test), and Duncan’s multiple range post hoc test. The level of statistical significance was considered P < 0.05. Oocytes were cultured in vitro maturation media (IVM) followed by in vitro fertilization (IVF), in vitro culture media 1 (IVC1), and in vitro culture media 2 (IVC2). Composition of the media was as follows: IVM medium was TCM-199 supplemented with 10% (v/v) fetal bovine serum, 1 µg mL−1 oestradiol-17β, 10 µg mL−1 FSH, 0.6 mM cysteine, and 0.2 mM sodium pyruvate. The IVC1 medium consisted of CR1-aa supplemented with 44 µg mL−1 sodium pyruvate, 14.6 µg mL−1 glutamine, 10 IU mL−1 penicillin, 0.1 mg mL−1 streptomycin, 3 mg mL−1 BSA, and 310 µg mL−1 glutathione. The IVC2 medium was the same composition as IVC1 except that BSA was replaced with 10% (v/v) fetal bovine serum. The final concentration of the optimized (0.5 µM) THF in culture medium was 0.4%. When coculturing with 0.5 µM THF in the IVM stage, the cleavage rate (58.65 ± 1.90% v. 56.87 ± 1.68%) was not significantly different, but the blastocyst rate (35.21 ± 1.44% v. 28.34 ± 2.11%) was significantly higher compared with the control group. The TUNEL assay confirmed that apoptotic nuclei in THF group were significantly reduced compared with the control group (2.32 ± 0.14 v. 5.65 ± 0.12). The total cell number of trophectoderm (TE) in control and THF groups was 115.34 ± 0.98 and 132.13 ± 1.55, and that of the inner cell mass (ICM) was 29.67 ± 0.40 and 39.94 ± 0.44, respectively. However, the ICM:TE ratio in control and treated blastocysts was 1:3.34 and 1:3.9, which was not statistically significant. Immunocytochemistry analysis (using antibodies to IKBKB, NFkB, COX2, CASP9, and CASP3) demonstrated that THF supplementation significantly attenuated expression of these proteins. The quantitative recerse transcription PCR data established that relative mRNA expression level of the anti-apoptotic gene BCL2 was up-regulated, whereas that of COX2, iNOS, BAX, IKBKB, NFkB, CASP9, and CASP3 were significantly down-regulated in the THF treated group compared with the control. In conclusion, 0.5 µM THF supplement in the IVM media did not have injurious effects on in vitro-cultured bovine embryos. This work was supported by grant from the Next-Generation BiogGeen21 (No. PJ01107703), IPET (No. 315017-5 and 117029-3), Allergy free cat (Co.. Felix Pets) and BK21plus.

scholarly journals A Systematic Comparative Study of the Toxicity of Semiconductor and Graphitic Carbon-Based Quantum Dots Using In Vitro Cell Models

2020 ◽  
Vol 10 (24) ◽  
pp. 8845
Author(s):  
Maria Carmen Navarro-Ruiz ◽  
Angelina Cayuela ◽  
María Laura Soriano ◽  
Rocio Guzmán-Ruiz ◽  
Maria M. Malagón ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cell Viability ◽  
Serum Proteins ◽  
Fetal Bovine Serum ◽  
Carbon Quantum Dots ◽  
Graphitic Carbon ◽  
Cell Models ◽  
Minimal Concentration ◽  
Fetal Bovine

A comparative, fully parallel study of nanoparticles (NPs) toxicity by in vitro cell viability is shown looking for reliable comparability of nanotoxicological results, a well-recognized bottleneck in the context. This procedure is suitable to compare toxicity of similar NPs, as well as the influence on toxicity of the size, surface, and other characteristics. As a case of study, semiconductor (SQDs) and graphitic-carbon quantum dots (CQDs) with identical surface groups and size were evaluated. All experiments were conducted at same conditions, involving two types of cells (mouse fibroblasts (3T3-L1) and carcinoma human hepatocellular cells (HepG2)) and different extracellular components (in the absence or presence of fetal bovine serum (FBS)). Cell viability demonstrated the excellent biocompatibility of CQDs compared to SQDs, which caused higher percentage of cell death at lower concentrations, as predicted but never clearly demonstrated. However, our comparative studies established that the toxicity of SQDs and CQDs are cellular type-dependent, and the absence or presence of serum proteins reduces the minimal concentration necessary of NPs to produce toxicity.

Fetal Bovine Serum: The Impact of Geography

2013 ◽  
Vol 12 (3) ◽  
pp. 28-30 ◽  
Author(s):  
William Siegel ◽  
Leland Foster
Keyword(s):  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine ◽  
The Impact

Addressing the impact of different fetal bovine serum percentages on mesenchymal stem cells biological performance

2019 ◽  
Vol 46 (4) ◽  
pp. 4437-4441 ◽  
Author(s):  
Ramada R. Khasawneh ◽  
Ahmed Hesham Al Sharie ◽  
Ejlal Abu-El Rub ◽  
Abdullah Omar Serhan ◽  
Hayam Nizar Obeidat
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine ◽  
Biological Performance ◽  
The Impact

Involvement of nucleus in the maturation of dengue-2 virus in C6/36 cells

1990 ◽  
Vol 48 (3) ◽  
pp. 912-913
Author(s):  
Jaang J. Wang ◽  
Cheng C. Chen ◽  
Men F. Shaio ◽  
Chia T. Liu ◽  
Chung S. Lee ◽  
...  
Keyword(s):  
Culture Medium ◽  
Cytopathic Effect ◽  
Fetal Bovine Serum ◽  
Virus Particles ◽  
Electron Microscopies ◽  
Embedding Technique ◽  
Fetal Bovine ◽  
Flat Embedding ◽  
20 Nm ◽  
Labeling Method

The involvement of nucleus in the maturation processes of Dengue-2 virus in a mosquito cell line, C6/36 cells, has been identified by the electron microscopy and immunocytochemistry. The C6/36 cells were obtained from ATCC and maintained in MEM culture medium containing 10% fetal bovine serum at 28°C. The cell suspensions or cells grown on teflon-coated coverslips were infected with Dengue-2 virus (107/ml) for various time periods of 2 hours, 3, 6, 8, and 10 days. The cells were then fixed in buffered 1.5% glutaraldehyde, and washed in acetone before immunolabeled with monoclonal antibody. An indirect immunocytochemical labeling method of avidin-biotin complex (ABC) conjugated with peroxidase or gold particles (20 nm in diameter) and a flat embedding technique were used to localize the virus particles.At early stages of infections (before 3 days), there were no virion particles detected. After 6 days and on of infections, cytopathic effect (CPE) was observed and showed positive immuno-peroxidase reactions under the light and electron microscopies.

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