Rapid detection of Mycobacterium tuberculosis cultures by direct ambient corona discharge ionization mass spectrometry of volatile metabolites

RSC Advances ◽  
2016 ◽  
Vol 6 (64) ◽  
pp. 59749-59752 ◽  
Author(s):  
Konstantin Chingin ◽  
Juchao Liang ◽  
Yanling Liu ◽  
Linfei Chen ◽  
Xiaoping Wu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mycobacterium Tuberculosis ◽  
Corona Discharge ◽  
High Throughput ◽  
Rapid Detection ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
High Chemical ◽  
Volatile Metabolites ◽  
Ms Analysis

High-throughput TB screening with high chemical specificity is achieved using direct ambient corona discharge ionization MS analysis of volatile metabolites.

scholarly journals Rapid detection of adulterated drugs in herbal dietary supplements by wooden-tip electrospray ionization mass spectrometry

2016 ◽  
Vol 8 (38) ◽  
pp. 6840-6846 ◽  
Author(s):  
Bin Hu ◽  
Yingyan Huang ◽  
Guo Yin ◽  
Gaofei Zhang ◽  
Liyang Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Dietary Supplements ◽  
Electrospray Ionization ◽  
High Throughput ◽  
Electrospray Ionization Mass Spectrometry ◽  
Rapid Detection ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass

High-throughput wooden-tip electrospray ionization mass spectrometry for rapid detection of adulterated drugs in herbal dietary supplements.

scholarly journals Development of a Novel Label-Free and High-Throughput Arginase-1 Assay Using Self-Assembled Monolayer Desorption Ionization Mass Spectrometry

2021 ◽  
pp. 247255522110006
Author(s):  
Michael D. Scholle ◽  
Zachary A. Gurard-Levin
Keyword(s):  
Mass Spectrometry ◽  
Drug Discovery ◽  
High Throughput ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Label Free ◽  
Desorption Ionization ◽  
Arginase 1 ◽  
Self Assembled ◽  
High Throughput Assay

Arginase-1, an enzyme that catalyzes the reaction of L-arginine to L-ornithine, is implicated in the tumor immune response and represents an interesting therapeutic target in immuno-oncology. Initiating arginase drug discovery efforts remains a challenge due to a lack of suitable high-throughput assay methodologies. This report describes the combination of self-assembled monolayers and matrix-assisted laser desorption ionization mass spectrometry to enable the first label-free and high-throughput assay for arginase activity. The assay was optimized for kinetically balanced conditions and miniaturized, while achieving a robust assay (Z-factor > 0.8) and a significant assay window [signal-to-background ratio > 20] relative to fluorescent approaches. To validate the assay, the inhibition of the reference compound nor-NOHA (Nω-hydroxy-nor-L-arginine) was evaluated, and the IC50 measured to be in line with reported results (IC50 = 180 nM). The assay was then used to complete a screen of 175,000 compounds, demonstrating the high-throughput capacity of the approach. The label-free format also eliminates opportunities for false-positive results due to interference from library compounds and optical readouts. The assay methodology described here enables new opportunities for drug discovery for arginase and, due to the assay flexibility, can be more broadly applicable for measuring other amino acid–metabolizing enzymes.

Rapid detection of drugs in biofluids using atmospheric pressure chemi/chemical ionization mass spectrometry

2009 ◽  
Vol 23 (3) ◽  
pp. 333-339 ◽  
Author(s):  
Lee Chuin Chen ◽  
Yutaka Hashimoto ◽  
Hiroko Furuya ◽  
Kenichi Takekawa ◽  
Takeo Kubota ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atmospheric Pressure ◽  
Rapid Detection ◽  
Chemical Ionization ◽  
Ionization Mass Spectrometry ◽  
Chemical Ionization Mass Spectrometry ◽  
Ionization Mass

High-throughput quantification of sodium saccharin in foods by ambient flame ionization mass spectrometry

Talanta ◽  
2018 ◽  
Vol 182 ◽  
pp. 241-246 ◽  
Author(s):  
Zhongquan Li ◽  
Fang Zhang ◽  
Junbo Zhao ◽  
Xiaopan Liu ◽  
Xiuping Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Flame Ionization ◽  
Sodium Saccharin
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