Removal efficiency of MIEX® pretreatment on typical proteins and amino acids derived from Microcystis aeruginosa

RSC Advances ◽  
2016 ◽  
Vol 6 (65) ◽  
pp. 60869-60876 ◽  
Author(s):  
Cheng Liu ◽  
Siyuan He ◽  
Zhehao Sun ◽  
Jie Wang ◽  
Wei Chen
Keyword(s):  
Amino Acids ◽  
Microcystis Aeruginosa ◽  
Removal Efficiency

Main categories of typical proteins and amino acids derived fromM. aeruginosawere identified and their removal by MIEX® was discussed.

ISOLATION AND IDENTIFICATION OF THE FAST-DEATH FACTOR IN MICROCYSTIS AERUGINOSA NRC-1

1959 ◽  
Vol 37 (1) ◽  
pp. 453-471 ◽  
Author(s):  
C. T. Bishop ◽  
E. F. L. J. Anet ◽  
P. R. Gorham
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Body Weight ◽  
Microcystis Aeruginosa ◽  
Sodium Salt ◽  
Cyclic Peptide ◽  
Isolation And Identification ◽  
Toxic Compound ◽  
Molar Ratios ◽  
Crude Preparation

The fast-death factor in Microcystis aeruginosa NRC-1 is an acidic, probably cyclic peptide containing the following amino acids in the molar ratios indicated: L-aspartic (1); L-glutamic (2); D-serine (1); L-valine (1); L-ornithine (1); L-alanine (2); L-leucine (2). It is possible, although not likely, that one of the residues of glutamic, alanine, or leucine also is in the D-configuration. The toxin, in the form of its sodium salt, was extracted from lyophilized algal cells by water, separated from pigments by extraction into n-butanol, and freed from high-molecular-weight impurities by dialysis. No separation of a single toxic compound could be obtained by countercurrent distribution, chromatography, or electrophoresis in carbonate, acetate, or phosphate buffers. Electrophoresis of the crude toxin on cellulose in 0.1 M borate yielded five peptides one of which was toxic and accounted for 100% of the toxicity present in the crude preparation. The intraperitoneal LD50of the pure toxin for mice was 0.466 ± 0.013 mg/kg body weight.

scholarly journals A formal synthesis of Balgacyclamide A using solution phase fragments condensation.

2020 ◽  
Author(s):  
Sandip Radhakisan Ugale ◽  
Somnath S Gholap
Keyword(s):  
Amino Acids ◽  
Total Synthesis ◽  
Spectral Data ◽  
Microcystis Aeruginosa ◽  
Solution Phase ◽  
Formal Synthesis ◽  
H Nmr ◽  
The Core ◽  
C Nmr

Abstract: A formal total synthesis of Balgacyclamide A as an antimalarial cynobactin of Microcystis aeruginosa (EAWAG 251) has been described. The synthesis of titled cyclamide was accomplished by the solution phase fragment synthesis using protection, deprotection and macrocylization process. Four common amino acids such as d-alanine, l-threonine, l-valine and d-allo-isoleucine, has been used for the construction of Balgacyclamide A. Including, the oxazoline and thiazole are the core structures was successfully achieved by using Burgess reagent and Hantzsch methods. The overall yield of the synthesized balgacyclamide A was found to be 2.03%, also structure was confirmed by1H-NMR, 13C-NMR and HRMS spectral data.

Effects of amino acids on microcystin production of the Microcystis aeruginosa

2009 ◽  
Vol 161 (2-3) ◽  
pp. 730-736 ◽  
Author(s):  
Ruihua Dai ◽  
Huijuan Liu ◽  
Jiuhui Qu ◽  
Xu Zhao ◽  
Yining Hou
Keyword(s):  
Amino Acids ◽  
Microcystis Aeruginosa ◽  
Microcystin Production

Removal Characteristics of Microcystis aeruginosa with One Brevibacillus parabrevis Strain

2010 ◽  
Vol 113-116 ◽  
pp. 928-931
Author(s):  
Rui Min Mu ◽  
Xue Liang Yuan ◽  
Gui Xia Ma
Keyword(s):  
16S Rdna ◽  
Microcystis Aeruginosa ◽  
Removal Efficiency ◽  
Water Body ◽  
Gene Sequence ◽  
Physiological Characteristics ◽  
16S Rdna Gene ◽  
Rdna Gene ◽  
Algicidal Bacterium ◽  
Brevibacillus Parabrevis

In this study, one strain of algicidal bacterium H01 was selected from the water body polluted by Microcystis aeruginosa. It had good removal effects on Microcystis aeruginosa and the removal characteristics on Microcystis aeruginosa were studied. The results indicated that the removal efficiency was connected with such factors as temperature and pH. And the experiment on algicidal mechanism indicated that it lysed the algae cells by secreting metabolites. According to the analysis of its physiological characteristics and 16S rDNA gene sequence, the strain H01 was identified as Brevibacillus parabrevis.

Coagulation performance and floc properties of Microcystis aeruginosa in the presence of humic acid

2014 ◽  
Vol 15 (2) ◽  
pp. 339-347
Author(s):  
Peixia Cheng ◽  
Fei Ge ◽  
Xingwang Liu ◽  
Xiaoshuang Zeng ◽  
Biao Chen
Keyword(s):  
Humic Acid ◽  
Microcystis Aeruginosa ◽  
Removal Efficiency ◽  
High Concentration ◽  
Polyaluminum Chloride ◽  
Charge Neutralization ◽  
Low Concentration ◽  
Chl A ◽  
Coagulation Performance ◽  
Floc Properties

Coagulation removal of algae in raw water could be significantly affected by humic acid (HA). A series of jar-tests were conducted to investigate the coagulation performance with polyaluminum chloride and floc properties of Microcystis aeruginosa, a unicellular cyanobacteria, in the presence of HA. Meanwhile the coagulation mechanism was explored through the measurement of zeta potential of the supernatant. The results showed that an optimal removal efficiency of chlorophyll-a (Chl-a) was obtained at pH 8.0 with a low concentration of HA (2 mg/L) and at pH 6.0 with a high concentration of HA (8 mg/L). The floc structure was more compact and bigger-sized and the calculated fractal dimension (Df) was larger at maximum coagulation efficiency. The variation of Df was consistent with that of Chl-a removal efficiency under the same coagulation conditions. Charge neutralization was inferred to be the dominant mechanism to remove algal cells with low concentration of HA, while charge neutralization, gathering and the bridging process worked together to remove algal cells with a high concentration of HA. These results provide insight on how to achieve an optimal removal efficiency of algae in the presence of different concentrations of HA in water treatment.

ISOLATION AND IDENTIFICATION OF THE FAST-DEATH FACTOR IN MICROCYSTIS AERUGINOSA NRC-1

1959 ◽  
Vol 37 (3) ◽  
pp. 453-471 ◽  
Author(s):  
C. T. Bishop ◽  
E. F. L. J. Anet ◽  
P. R. Gorham
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Body Weight ◽  
Microcystis Aeruginosa ◽  
Sodium Salt ◽  
Cyclic Peptide ◽  
Isolation And Identification ◽  
Toxic Compound ◽  
Molar Ratios ◽  
Crude Preparation

The fast-death factor in Microcystis aeruginosa NRC-1 is an acidic, probably cyclic peptide containing the following amino acids in the molar ratios indicated: L-aspartic (1); L-glutamic (2); D-serine (1); L-valine (1); L-ornithine (1); L-alanine (2); L-leucine (2). It is possible, although not likely, that one of the residues of glutamic, alanine, or leucine also is in the D-configuration. The toxin, in the form of its sodium salt, was extracted from lyophilized algal cells by water, separated from pigments by extraction into n-butanol, and freed from high-molecular-weight impurities by dialysis. No separation of a single toxic compound could be obtained by countercurrent distribution, chromatography, or electrophoresis in carbonate, acetate, or phosphate buffers. Electrophoresis of the crude toxin on cellulose in 0.1 M borate yielded five peptides one of which was toxic and accounted for 100% of the toxicity present in the crude preparation. The intraperitoneal LD50of the pure toxin for mice was 0.466 ± 0.013 mg/kg body weight.

A new isolation and structure for the endotoxin from Microcystis aeruginosa NRC-1

1970 ◽  
Vol 48 (4) ◽  
pp. 508-510 ◽  
Author(s):  
J. Rama Murthy ◽  
J. B. Capindale
Keyword(s):  
Amino Acids ◽  
Body Weight ◽  
Solvent Extraction ◽  
Microcystis Aeruginosa ◽  
Green Alga ◽  
Complex Structure ◽  
Strain Analysis ◽  
Blue Green Alga ◽  
Algal Strain ◽  
Toxic Material

The endotoxin from the blue–green alga Microcystis aeruginosa NRC-1 has been isolated as its ammonium salt by a new procedure involving solvent extraction and elution from DEAE-Sephadex A-25. The intraperitoneal LD100 of this toxin preparation for mice was 0.1 mg/kg body weight. The product is a white solid which appears to be chromatographically and electrophoretically homogeneous. This toxin produces similar symptoms in mice to those described originally for the toxic material from the same algal strain. Analysis of the toxin hydrolysate indicates a more complex structure since seven more amino acids have been found, including tyrosine, proline, and arginine, in addition to the seven reported before.

Nontoxic and toxic oligopeptides with D-amino acids and unusual residues in Microcystis aeruginosa PCC 7806

1989 ◽  
Vol 151 (5) ◽  
pp. 411-415 ◽  
Author(s):  
I. M. Birk ◽  
R. Dierstein ◽  
I. Kaiser ◽  
U. Matern ◽  
W. A. König ◽  
...  
Keyword(s):  
Amino Acids ◽  
Microcystis Aeruginosa
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