Characterization and sorting of cells based on stiffness contrast in a microfluidic channel

RSC Advances ◽  
2016 ◽  
Vol 6 (78) ◽  
pp. 74704-74714 ◽  
Author(s):  
P. Sajeesh ◽  
A. Raj ◽  
M. Doble ◽  
A. K. Sen
Keyword(s):  
Microfluidic Device ◽  
Microfluidic Channel

This paper reports the characterization and sorting of cells based on stiffness contrast. A microfluidic device with focusing and spacing control for stiffness based sorting of cells is designed, fabricated and demonstrated.

Magnetophoresis ‘meets’ viscoelasticity: deterministic separation of magnetic particles in a modular microfluidic device

Lab on a Chip ◽  
2015 ◽  
Vol 15 (8) ◽  
pp. 1912-1922 ◽  
Author(s):  
Francesco Del Giudice ◽  
Hojjat Madadi ◽  
Massimiliano M. Villone ◽  
Gaetano D'Avino ◽  
Angela M. Cusano ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Magnetic Particles ◽  
Magnetic Beads ◽  
Microfluidic Channel

Deflection of magnetic beads in a microfluidic channel can be improved through viscoelastic focusing.

Particle Manipulation Inside a Grooved Microfluidic Channel

2009 ◽  
Author(s):  
Hsiu-hung Chen ◽  
Dayong Gao
Keyword(s):  
Rapid Prototyping ◽  
Microfluidic Device ◽  
Microfluidic Channel ◽  
Lab On A Chip ◽  
Cell Suspensions ◽  
Microfluidic Channels ◽  
Particle Manipulation ◽  
Post Processing ◽  
Processing Stage ◽  
Submicron Structures

The manipulation of particles and cells in micro-fluids, such as cell suspensions, is a fundamental task in Lab-on-a-Chip applications. According to their analysis purposes in either the pre- or post-processing stage, particles/cells flowing inside a microfluidic channel are handled by means of enriching, trapping, separating or sorting. In this study, we report the use of patterning flows produced by a series of grooved surfaces with different geometrical setups integrated into a microfluidic device, to continuously manipulate the flowing particles (5 to 20 μm in diameters) of comparable sizes to the depth of the channel in ways of: 1) concentrating, 2) focusing, and 3) potential separating. The device is fabricated using soft lithographic techniques and is composed of inlets, microfluidic channels, and outlets for loading, manipulating and retrieving cell suspensions, respectively. Such fabrication methods allow rapid prototyping of micron or submicron structures with multiple layers and replica molding on those fabricated features in a clear polymer. The particles are evenly distributed in the entrance of the microchannel and illustrate the enriching, focusing, or size-selective profiles after passing through the patterning grooves. We expect that the techniques of manipulating cell suspensions from this study can facilitate the development of cell-based devices on 1) the visualization of counting, 2) the visualization of sizing, and 3) the particle separating.

scholarly journals Single microfluidic fuel cell with three fuels – formic acid, glucose and microbes: A comparative performance investigation

2021 ◽  
Vol 11 (4) ◽  
pp. 306-316
Author(s):  
Sanket Goel ◽  
Lanka Tata Rao ◽  
Prakash Rewatkar ◽  
Haroon Khan ◽  
Satish Kumar Dubey ◽  
...  
Keyword(s):  
Power Generation ◽  
Fuel Cells ◽  
Fuel Cell ◽  
Formic Acid ◽  
Microfluidic Device ◽  
Microfluidic Channel ◽  
Open Circuit ◽  
Portable Devices ◽  
Comparative Performance ◽  
Microfluidic Fuel Cell

The development of microfluidic and nanofluidic devices is gaining remarkable attention due to the emphasis put on miniaturization of conventional energy conversion and storage processes. A microfluidic fuel cell can integrate flow of electrolytes, electrode-electrolyte interactions, and power generation in a microfluidic channel. Such microfluidic fuel cells can be categorized on the basis of electrolytes and catalysts used for power generation. In this work, for the first time, a single microfluidic fuel cell was harnessed by using different fuels like glucose, microbes and formic acid. Herein, multi-walled carbon nanotubes (MWCNT) acted as electrode material, and performance investigations were carried out separately on the same microfluidic device for three different types of fuel cells (formic acid, microbial and enzymatic). The fabricated miniaturized microfluidic device was successfully used to harvest energy in microwatts from formic acid, microbes and glucose, without any metallic catalyst. The developed microfluidic fuel cells can maintain stable open-circuit voltage, which can be used for energizing various low-power portable devices or applications.

scholarly journals Design and Fabrication of a PDMS-Based Manual Micro-Valve System for Microfluidic Applications

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Tu N. Le ◽  
Van-Anh Nguyen ◽  
Giang L. Bach ◽  
Lam D. Tran ◽  
Ha H. Cao
Keyword(s):  
Microfluidic Device ◽  
Liquid Flow ◽  
Magnetic Nanoparticle ◽  
Microfluidic Channel ◽  
Reaction Chamber ◽  
Valve System ◽  
Dimethyl Siloxane ◽  
Flow Mixing ◽  
Liquid Flows ◽  
Micro Reactor

In this study, a manual micro-valve system (dimension: w × h: 1000 × 50 μm with 8-integrated channel valves was designed for controlling up to 8 different flows of agents (including magnetic nanoparticle flow) injected into the mixture zone of the microfluidics. The working parts of the micro-valve and microfluidic channel were fabricated from Poly(dimethyl siloxane) materials. The aperture of each channel valve was manually manipulated by a screw and a support kit (made of Plexiglas® materials). This valve system was connected to a microfluidic device with two important modules: a multi-liquid mixing component and a micro-reactor (~5 μL of volume). The study on controlling liquid flows proved that this valve system was effective for the experiments on the flow mixing and delivering the reactants into the micro-reaction chamber in order. The results are the first step for the fabrication of liquid flow controllers in integrated microfluidic systems towards biological analysis applications.

scholarly journals Development of an electrically responsive hydrogel for programmable in situ immobilization within a microfluidic device

Soft Matter ◽  
2021 ◽  
Author(s):  
Rok Ambrožič ◽  
Igor Plazl
Keyword(s):  
Microfluidic Device ◽  
Hybrid Material ◽  
Microfluidic Channel ◽  
Iron Ion ◽  
In Situ Immobilization ◽  
3D Network ◽  
In Situ Formation

A novel microfluidic channel device with programmable in situ formation of a hydrogel 3D network was designed. A biocompatible hybrid material consisting of iron ion-crosslinked alginate was used as the...

scholarly journals An Ultrarapid Method of Creating 3D Channels and Microstructures

2005 ◽  
Vol 10 (1) ◽  
pp. 24-28 ◽  
Author(s):  
Glennys Mensing ◽  
Thomas Pearce ◽  
David J. Beebe
Keyword(s):  
Liquid Phase ◽  
Microfluidic Device ◽  
Low Cost ◽  
Three Dimensional ◽  
Microfluidic Channel ◽  
Complex Geometries ◽  
Channel Networks ◽  
Functional Density ◽  
The Creation ◽  
Interconnecting Layers

We present a method of creating three dimensional microfluidic channel networks and freestanding microstructures using liquid phase photopolymerization techniques. The use of liquid phase microfabrication facilitates the creation of microstructured devices using low-cost materials and equipment. The ability to add multiple layers allows for complex geometries and increases the functional density of channeled devices. The multilayer technique provides a method of interconnecting layers or combining separate layers to form a truly integrated multilayered microfluidic device, as well as a means of forming multilayered freestanding structures. Because this method is based on the fundamentals of microfluidic tectonics (μFT), all components (valves, mixers, filters) compatible with μFT can be integrated into the multilayer channel networks.

scholarly journals Droplet movement control using fuzzy logic controller and image processing along with pin valves inside microfluidic device

2019 ◽  
Vol 52 (9-10) ◽  
pp. 1517-1531 ◽  
Author(s):  
Faisal Mehmood ◽  
Zeeshan Haider ◽  
Umar Farooq ◽  
Yin Baoqun
Keyword(s):  
Image Processing ◽  
Fuzzy Logic ◽  
Microfluidic Device ◽  
Fuzzy Logic Controller ◽  
Active Contours ◽  
Fuzzy Controller ◽  
Microfluidic Channel ◽  
Processing Algorithm ◽  
Image Processing Algorithm ◽  
Droplet Movement

Most of the research studies nowadays are trying to bring automation to biomedical engineering and Lab on a Chip which is fast growing interdisciplinary field and has attracted researchers from various fields. The objective of this paper is to present an overall system to control droplet movement inside microfluidic channel using fuzzy logic controller, image processing algorithm, and microvalves installed within microfluidic channel. A state space model has been derived from circuit analogy approach to describe the microfluidic network. Furthermore, a COMSOL-based study is primed for device structure by means of droplet generation and controlling the droplet through fitted valves. Moreover, an image processing algorithm based on active contours has been proposed in this research to track the movement of the droplet through the channel. This droplet controlling method is utterly based on fuzzy controller as well as camera images to move the droplet at desired position by controlling flow rates inside the fluidic channel using valves installed inside the microfluidic device. The results indicate that the fuzzy logic controller performs much better in terms of stability and faster response as compared to conventional proportional–integral–derivative controller.

scholarly journals Modular pumps as programmable hydraulic batteries for microfluidic devices

TECHNOLOGY ◽  
2017 ◽  
Vol 05 (01) ◽  
pp. 21-30 ◽  
Author(s):  
Brian M. Cummins ◽  
Rukesh Chinthapatla ◽  
Balaji Lenin ◽  
Frances S. Ligler ◽  
Glenn M. Walker
Keyword(s):  
Microfluidic Device ◽  
Microfluidic Channel ◽  
Microfluidic Devices ◽  
Complex Flow ◽  
Oscillating Flows ◽  
Flow Rates ◽  
Simple Fluid ◽  
Flow Profiles ◽  
Over Time

Simple fluid pumps have been developed to improve microfluidic device portability, but they cannot be easily programmed, produce repeatable pumping performance, or generate complex flow profiles — key requirements for increasing the functionality of portable microdevices. We present a detachable, paper-based, “hydraulic battery” that can be connected to the outlet of a microfluidic channel to pump fluid at varying flow rates over time, including step changes, ramping flows, and oscillating flows.

A Fully Integrated System for Cell/Particle Sorting in a Microfluidic Device Utilizing an Optical Tweezing and DIP Recognition Approach

2006 ◽  
Vol 505-507 ◽  
pp. 643-648 ◽  
Author(s):  
Yu Sheng Chien ◽  
Che Hsin Lin ◽  
Fu Jen Kao ◽  
Cheng Wen Ko
Keyword(s):  
Image Processing ◽  
Digital Image Processing ◽  
Microfluidic Device ◽  
Digital Image ◽  
Microfluidic Channel ◽  
Optical Tweezer ◽  
Processing Technique ◽  
Image Processing Technique ◽  
Target Cells ◽  
Digital Image Processing Technique

This paper proposes a novel microfluidic system for cell/microparticle recognition and manipulation utilizing a digital image processing technique (DIP) controlled optical tweezer under microfluidic configuration. Cell/microparticle samples are firstly electrokinetically sorted in a microfluidic channel and pass through an image detection region. Digital image processing technique is used to count and recognize the cell/particle samples and then sends control signals to generate laser pulses to manipulate the target cell/particles optically. The optical tweezer system is capable of catching, moving and switching the target cells within the microfluidic channel. The trapping force of the optical tweezer is also demonstrated utilizing the relationship between Stocks-drag force of microparticles and the applied electroosmotic flow. The proposed system provides a simple but high-performance solution for microparticle manipulation in a microfluidic device.

scholarly journals Stripping Material from a Supported Lipid Bilayer with High Speed Buffer Flow

2020 ◽  
Vol 17 (3) ◽  
pp. 51-59
Author(s):  
Michael Ornstead ◽  
Ruth Hunter ◽  
Mason Valentine ◽  
Cameron Cooper ◽  
Stephen Smith ◽  
...  
Keyword(s):  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Microfluidic Device ◽  
High Speed ◽  
Membrane Vesicle ◽  
Microfluidic Channel ◽  
Lipid Vesicles ◽  
Membrane Properties ◽  
Supported Lipid Bilayer ◽  
Glass Coverslip

A microfluidic device was created and used to demonstrate that supported lipid bilayers can be deposited on clean glass slides and removed using high velocity buffer flow (1-4 m/s linear velocity). This was accomplished by forcing the flow through a microfluidic channel covering an annealed glass coverslip bearing a supported lipid bilayer (SLB). The removal of bilayer material was monitored via fluorescence microscopy, and two basic regimes were observed: at 1-2 m/s smaller areas were stripped, while at 3-4 m/s larger areas were stripped. SLB removal was verified by two means. First, lipid vesicles labeled with a different fluorescent dye were added to the device and filled in holes left by the removal of the original SLB, allowing stripping to be verified visually. Second, the solutions obtained from stripping were concentrated and the fluorescence in the concentrates was measured. The ability to strip SLB from glass provides a relatively gentle method of creating spatially inhomogeneous SLB, which could be a useful tool in the continued investigation of membrane properties and components. KEYWORDS: Supported Lipid Bilayer; Membrane Vesicle; Microfluidic Device

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