Development of a novel H2S and GSH detection cocktail for fluorescence imaging

RSC Advances ◽  
2016 ◽  
Vol 6 (64) ◽  
pp. 59882-59888 ◽  
Author(s):  
Juan Cheng ◽  
Meng Liu ◽  
Baihao Shao ◽  
Shuai Zhang ◽  
Jia Li ◽  
...  

A highly sensitive fluorescent detection cocktail has been developed for the simultaneous imaging of H2S and GSH in live cells.

The Analyst ◽  
2021 ◽  
Vol 146 (20) ◽  
pp. 6297-6305
Author(s):  
Qinglan Miao ◽  
Ji Qi ◽  
Yuanyuan Li ◽  
Xinxia Fan ◽  
Dongmei Deng ◽  
...  

A novel paper-based chip that anchored zinc-doped carbon dots was constructed for sensitive and stable fluorescent detection of Cu2+. Zn doping increased the active sites for simplifying the modification of carbon dots.


Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1207
Author(s):  
Hong Jae Cheon ◽  
Quynh Huong Nguyen ◽  
Moon Il Kim

Inspired by the active site structure of natural horseradish peroxidase having iron as a pivotal element with coordinated histidine residues, we have developed histidine coated magnetic nanoparticles (His@MNPs) with relatively uniform and small sizes (less than 10 nm) through one-pot heat treatment. In comparison to pristine MNPs and other amino acid coated MNPs, His@MNPs exhibited a considerably enhanced peroxidase-imitating activity, approaching 10-fold higher in catalytic reactions. With the high activity, His@MNPs then were exploited to detect the important neurotransmitter acetylcholine. By coupling choline oxidase and acetylcholine esterase with His@MNPs as peroxidase mimics, target choline and acetylcholine were successfully detected via fluorescent mode with high specificity and sensitivity with the limits of detection down to 200 and 100 nM, respectively. The diagnostic capability of the method is demonstrated by analyzing acetylcholine in human blood serum. This study thus demonstrates the potential of utilizing His@MNPs as peroxidase-mimicking nanozymes for detecting important biological and clinical targets with high sensitivity and reliability.


2017 ◽  
Vol 58 (1) ◽  
pp. 30-34 ◽  
Author(s):  
Hongda Li ◽  
Pengcheng Zhao ◽  
Ning Zou ◽  
Hui Wang ◽  
Kaixuan Sun

Small ◽  
2017 ◽  
Vol 13 (27) ◽  
pp. 1700569 ◽  
Author(s):  
Peiyan Yuan ◽  
Xin Mao ◽  
Kok Chan Chong ◽  
Jiaqi Fu ◽  
Sijun Pan ◽  
...  

2013 ◽  
Vol 49 (90) ◽  
pp. 10599 ◽  
Author(s):  
Yan-Xia Qi ◽  
Min Zhang ◽  
Qian-Qian Fu ◽  
Ran Liu ◽  
Guo-Yue Shi

Author(s):  
Matthew K. Daddysman ◽  
Michael A. Tycon ◽  
Christopher J. Fecko

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