scholarly journals Synthetic approaches to nucleopeptides containing all four nucleobases, and nucleic acid-binding studies on a mixed-sequence nucleo-oligolysine

RSC Advances ◽  
2016 ◽  
Vol 6 (68) ◽  
pp. 63578-63585 ◽  
Author(s):  
Giovanni N. Roviello ◽  
Domenica Musumeci
Keyword(s):  
Nucleic Acid ◽  
Solid Phase ◽  
Nucleic Acid Binding ◽  
Binding Studies ◽  
Mixed Sequence ◽  
Synthetic Routes ◽  
Synthetic Approaches

In this article we describe two solid-phase synthetic routes to obtain a nucleo-oligolysine α-peptide containing all four natural nucleobases.

scholarly journals Correction: Synthetic approaches to nucleopeptides containing all four nucleobases, and nucleic acid-binding studies on a mixed-sequence nucleo-oligolysine

RSC Advances ◽  
2016 ◽  
Vol 6 (82) ◽  
pp. 78486-78486
Author(s):  
Giovanni N. Roviello ◽  
Domenica Musumeci
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Binding ◽  
Binding Studies ◽  
Mixed Sequence ◽  
Synthetic Approaches

Correction for ‘Synthetic approaches to nucleopeptides containing all four nucleobases, and nucleic acid-binding studies on a mixed-sequence nucleo-oligolysine’ by Giovanni N. Roviello et al., RSC Adv., 2016, 6, 63578–63585.

scholarly journals Synthesis and Applications of Porphyrin-Biomacromolecule Conjugates

2021 ◽  
Vol 9 ◽  
Author(s):  
Pravin Pathak ◽  
Mohammad Amin Zarandi ◽  
Xiao Zhou ◽  
Janarthanan Jayawickramarajah
Keyword(s):  
Nucleic Acid ◽  
Functional Groups ◽  
Self Assembly ◽  
De Novo ◽  
Solid Phase ◽  
Academic Research ◽  
Fine Tuning ◽  
Cancer Tissue ◽  
Site Specific ◽  
Synthetic Approaches

With potential applications in materials and especially in light-responsive biomedicine that targets cancer tissue selectively, much research has focused on developing covalent conjugation techniques to tether porphyrinoid units to various biomacromolecules. This review details the key synthetic approaches that have been employed in the recent decades to conjugate porphyrinoids with oligonucleotides and peptides/proteins. In addition, we provide succinct discussions on the subsequent applications of such hybrid systems and also give a brief overview of the rapidly progressing field of porphyrin-antibody conjugates. Since nucleic acid and peptide systems vary in structure, connectivity, functional group availability and placement, as well as stability and solubility, tailored synthetic approaches are needed for conjugating to each of these biomacromolecule types. In terms of tethering to ONs, porphyrins are typically attached by employing bioorthogonal chemistry (e.g., using phosphoramidites) that drive solid-phase ON synthesis or by conducting post-synthesis modifications and subsequent reactions (such as amide couplings, hydrazide-carbonyl reactions, and click chemistry). In contrast, peptides and proteins are typically conjugated to porphyrinoids using their native functional groups, especially the thiol and amine side chains. However, bioorthogonal reactions (e.g., Staudinger ligations, and copper or strain promoted alkyne-azide cycloadditions) that utilize de novo introduced functional groups onto peptides/proteins have seen vigorous development, especially for site-specific peptide-porphyrin tethering. While the ON-porphyrin conjugates have largely been explored for programmed nanostructure self-assembly and artificial light-harvesting applications, there are some reports of ON-porphyrin systems targeting clinically translational applications (e.g., antimicrobial biomaterials and site-specific nucleic acid cleavage). Conjugates of porphyrins with proteinaceous moieties, on the other hand, have been predominantly used for therapeutic and diagnostic applications (especially in photodynamic therapy, photodynamic antimicrobial chemotherapy, and photothermal therapy). The advancement of the field of porphyrinoid-bioconjugation chemistry from basic academic research to more clinically targeted applications require continuous fine-tuning in terms of synthetic strategies and hence there will continue to be much exciting work on porphyrinoid-biomacromolecule conjugation.

scholarly journals Crystal structure of an Escherichia coli Hfq Core (residues 2–69)–DNA complex reveals multifunctional nucleic acid binding sites

2020 ◽  
Vol 48 (7) ◽  
pp. 3987-3997 ◽  
Author(s):  
Jillian Orans ◽  
Alexander R Kovach ◽  
Kirsten E Hoff ◽  
Nicola M Horstmann ◽  
Richard G Brennan
Keyword(s):  
Crystal Structure ◽  
Escherichia Coli ◽  
Nucleic Acid ◽  
Dna Interaction ◽  
Dna Duplexes ◽  
Nucleic Acid Binding ◽  
Binding Studies ◽  
Bacterial Gene ◽  
Bacterial Gene Expression ◽  
Microscopy Data

Abstract Hfq regulates bacterial gene expression post-transcriptionally by binding small RNAs and their target mRNAs, facilitating sRNA-mRNA annealing, typically resulting in translation inhibition and RNA turnover. Hfq is also found in the nucleoid and binds double-stranded (ds) DNA with a slight preference for A-tracts. Here, we present the crystal structure of the Escherichia coli Hfq Core bound to a 30 bp DNA, containing three 6 bp A-tracts. Although previously postulated to bind to the ‘distal’ face, three statistically disordered double stranded DNA molecules bind across the proximal face of the Hfq hexamer as parallel, straight rods with B-DNA like conformational properties. One DNA duplex spans the diameter of the hexamer and passes over the uridine-binding proximal-face pore, whereas the remaining DNA duplexes interact with the rims and serve as bridges between adjacent hexamers. Binding is sequence-independent with residues N13, R16, R17 and Q41 interacting exclusively with the DNA backbone. Atomic force microscopy data support the sequence-independent nature of the Hfq-DNA interaction and a role for Hfq in DNA compaction and nucleoid architecture. Our structure and nucleic acid-binding studies also provide insight into the mechanism of sequence-independent binding of Hfq to dsRNA stems, a function that is critical for proper riboregulation.

Conformational and Nucleic Acid Binding Studies on the Synthetic Nucleocapsid Protein of HIV-1

1993 ◽  
Vol 229 (1) ◽  
pp. 94-104 ◽  
Author(s):  
Andrej Surovoy ◽  
Jens Dannull ◽  
Karin Moelling ◽  
Gunther Jung
Keyword(s):  
Nucleic Acid ◽  
Nucleocapsid Protein ◽  
Nucleic Acid Binding ◽  
Binding Studies ◽  
Hiv 1

Synthesis and nucleic acid binding studies of novel pyrrolidinyl PNA carrying an N-amino-N-methylglycine spacer

2003 ◽  
Vol 44 (8) ◽  
pp. 1663-1666 ◽  
Author(s):  
Tirayut Vilaivan ◽  
Chaturong Suparpprom ◽  
Preeyanut Duanglaor ◽  
Pongchai Harnyuttanakorn ◽  
Gordon Lowe
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Binding ◽  
Binding Studies

Synthesis, characterization and nucleic acid binding studies of mononuclear copper(II) complexes derived from azo containing O, O donor ligands

2018 ◽  
Vol 37 (10) ◽  
pp. 563-584 ◽  
Author(s):  
Mamta Tripathi ◽  
Chandra Gopal Giri ◽  
Devashish Das ◽  
Rama Pande ◽  
Sougata Sarkar ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Donor Ligands ◽  
Nucleic Acid Binding ◽  
Binding Studies
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