Encapsulation of a photosensitizer into cell membrane capsules for photodynamic therapy

RSC Advances ◽  
2016 ◽  
Vol 6 (43) ◽  
pp. 37212-37220 ◽  
Author(s):  
Lijie Han ◽  
Ying Chen ◽  
Jie Niu ◽  
Lihua Peng ◽  
Zhengwei Mao ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Photodynamic Therapy ◽  
Cell Membrane ◽  
Sustained Release ◽  
Enzymatic Reduction ◽  
Reduced Toxicity ◽  
Enhanced Stability

CMCs were used to encapsulate MB (CMCs@MB) using temporary permeation of the plasma membrane and resealing. Encapsulation in the CMCs leads to sustained release of MB with enhanced stability against enzymatic reduction and reduced toxicity.

scholarly journals Red Blood Cell Membrane-Camouflaged Tedizolid Phosphate-Loaded PLGA Nanoparticles for Bacterial-Infection Therapy

Pharmaceutics ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 99
Author(s):  
Xinyi Wu ◽  
Yichen Li ◽  
Faisal Raza ◽  
Xuerui Wang ◽  
Shulei Zhang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Membrane ◽  
Blood Cell ◽  
Sustained Release ◽  
Delivery System ◽  
Red Blood Cell ◽  
Multiple Drug Resistance ◽  
Plga Nanoparticles ◽  
Multiple Drug ◽  
Red Blood Cell Membrane

Multiple drug resistance (MDR) in bacterial infections is developed with the abuse of antibiotics, posing a severe threat to global health. Tedizolid phosphate (TR-701) is an efficient prodrug of tedizolid (TR-700) against gram-positive bacteria, including methicillin-sensitive staphylococcus aureus (MSSA) and methicillin-resistant staphylococcus aureus (MRSA). Herein, a novel drug delivery system: Red blood cell membrane (RBCM) coated TR-701-loaded polylactic acid-glycolic acid copolymer (PLGA) nanoparticles (RBCM-PLGA-TR-701NPs, RPTR-701Ns) was proposed. The RPTR-701Ns possessed a double-layer core-shell structure with 192.50 ± 5.85 nm in size, an average encapsulation efficiency of 36.63% and a 48 h-sustained release in vitro. Superior bio-compatibility was confirmed with red blood cells (RBCs) and HEK 293 cells. Due to the RBCM coating, RPTR-701Ns on one hand significantly reduced phagocytosis by RAW 264.7 cells as compared to PTR-701Ns, showing an immune escape effect. On the other hand, RPTR-701Ns had an advanced exotoxins neutralization ability, which helped reduce the damage of MRSA exotoxins to RBCs by 17.13%. Furthermore, excellent in vivo bacteria elimination and promoted wound healing were observed of RPTR-701Ns with a MRSA-infected mice model without causing toxicity. In summary, the novel delivery system provides a synergistic antibacterial treatment of both sustained release and bacterial toxins absorption, facilitating the incorporation of TR-701 into modern nanotechnology.

scholarly journals Photodynamic Therapy: Targeting Cancer Biomarkers for the Treatment of Cancers

Cancers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2992
Author(s):  
Xinning Wang ◽  
Dong Luo ◽  
James P. Basilion
Keyword(s):  
Photodynamic Therapy ◽  
Targeted Delivery ◽  
Cancer Biomarkers ◽  
Malignant Cell ◽  
Minimal Invasiveness ◽  
Normal Tissues ◽  
Effective Treatment Modality ◽  
Tissue Selectivity ◽  
Reduced Toxicity ◽  
Local Illumination

Photodynamic therapy (PDT) is a well-documented therapy that has emerged as an effective treatment modality of cancers. PDT utilizes harmless light to activate non- or minimally toxic photosensitizers to generate cytotoxic species for malignant cell eradication. Compared with conventional chemotherapy and radiotherapy, PDT is appealing by virtue of the minimal invasiveness, its safety, as well as its selectivity, and the fact that it can induce an immune response. Although local illumination of the cancer lesions renders intrinsic selectivity of PDT, most photosensitizers used in PDT do not display significant tumor tissue selectivity. There is a need for targeted delivery of photosensitizers. The molecular identification of cancer antigens has opened new possibilities for the development of effective targeted therapy for cancer patients. This review provides a brief overview of recent achievements of targeted delivery of photosensitizers to cancer cells by targeting well-established cancer biomarkers. Overall, targeted PDT offers enhanced intracellular accumulation of the photosensitizer, leading to improved PDT efficacy and reduced toxicity to normal tissues.

PEGylated TNF-related apoptosis-inducing ligand (TRAIL)-loaded sustained release PLGA microspheres for enhanced stability and antitumor activity

2011 ◽  
Vol 150 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Tae Hyung Kim ◽  
Hai Hua Jiang ◽  
Chan Woong Park ◽  
Yu Seok Youn ◽  
Seulki Lee ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Sustained Release ◽  
Plga Microspheres ◽  
Enhanced Stability

Time-Course of Changes in the Plasma–Membrane Lipid Bilayer and Cellular Ultrastructure Induced by Tumour Necrosis Factor-α in K 562 and Daudi Cells

1995 ◽  
Vol 23 (4) ◽  
pp. 254-263 ◽  
Author(s):  
M Marutaka ◽  
H Iwagaki ◽  
K Mizukawa ◽  
N Tanaka ◽  
K Orita
Keyword(s):  
Plasma Membrane ◽  
Cell Membrane ◽  
Membrane Fluidity ◽  
Time Course ◽  
Membrane Lipid ◽  
Plasma Membrane Lipid ◽  
Membrane Lipid Bilayer ◽  
Cell Membrane Fluidity ◽  
K 562 Cells ◽  
Factor Α

The time-course of changes in the plasma-membrane lipid bilayer induced by tumour necrosis factor-α (TNF) were investigated in cultured cells using spin-label electron-spin-resonance techniques. Treatment of K 562 cells, a human chronic myelocytic leukaemia cell line, in suspension culture with TNF for up to 6 h caused an initial increase in cell-membrane fluidity, which returned to the control level after 12 h of treatment. After 24 h of treatment, the cell-membrane fluidity had decreased and this decrease was maintained after 48 h of treatment. In Daudi cells, a human malignant lymphoma cell line, TNF, did not induce any changes in cell-membrane fluidity, indicating that the effect of TNF on membrane structure is cell-specific. The early and transient change in membrane fluidity in K 562 cells is probably related to signal generation, while the later, persistent change may reflect the phenotype of TNF-treated cells, in particular, changes in the plasma membrane-cytoplasmic complex. Histochemical electron microscopic studies indicated that the membrane fluidity changes induced by TNF have an ultrastructural correlate.

scholarly journals Comparative Roles of the Cell Wall and Cell Membrane in Limiting Uptake of Xenobiotic Molecules by Saccharomyces cerevisiae

2003 ◽  
Vol 47 (6) ◽  
pp. 2012-2014 ◽  
Author(s):  
Mustapha Aouida ◽  
Omar Tounekti ◽  
Omrane Belhadj ◽  
Lluis M. Mir
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Plasma Membrane ◽  
Cell Membrane ◽  
Membrane Protein

ABSTRACT Using reversible electropermeabilization of cells and spheroplasts, we show that the cell wall and plasma membrane partly account for bleomycin resistance by acting as two independent barriers. We also report on the presence of a membrane protein that may be responsible for bleomycin internalization and toxicity in Saccharomyces cerevisiae.

scholarly journals Cation-chloride cotransporters, Na/K pump, and channels in cell water and ions regulation: in silico and experimental studies of the U937 cells under stopping the pump and during RVD

2021 ◽  
Author(s):  
Alexey A Vereninov ◽  
Valentina Yurinskaya
Keyword(s):  
Plasma Membrane ◽  
Cell Membrane ◽  
Real Time ◽  
Intracellular Signaling ◽  
Ion Homeostasis ◽  
Regulatory Volume Decrease ◽  
Experimental Studies ◽  
U937 Cells ◽  
Ionic Homeostasis ◽  
Monovalent Ions

Cation-coupled chloride cotransporters play a key role in generating the Cl− electrochemical gradient on the cell membrane which is important for regulation of many cellular processes. However, the cooperation of transporters and channels of the plasma membrane in holding the ionic homeostasis of the whole cell remains poorly characterized because of the lack of a suitable tool for its computation. Our software successfully predicted in real-time changes in the ion homeostasis of U937 cells after stopping the Na/K pump, but so far considered the model with only NC cotransporter. Here the model with all main types of cotransporters is used in computation of the rearrangements of ionic homeostasis due to stopping the pump and associated with the regulatory volume decrease (RVD) of cells swollen in hypoosmolar medium. The parameters obtained for the real U937 cells are used. Successful prediction of changes in ion homeostasis in real-time after stopping the pump using the model with all major cotransporters indicates that the model is reliable. Using this model for analysis RVD showed that there is a "physical" RVD, associated with the time-dependent changes in electrochemical ion gradients, but not with alteration of channels and transporters of the plasma membrane that should be considered in studies of truly active regulatory processes mediated by the intracellular signaling network. The developed software can be useful for calculation of the balance of the partial unidirectional fluxes of monovalent ions across the cell membrane of various cells under various conditions.

Mitochondria and plasma membrane dual-targeted chimeric peptide for single-agent synergistic photodynamic therapy

Biomaterials ◽  
2019 ◽  
Vol 188 ◽  
pp. 1-11 ◽  
Author(s):  
Hong Cheng ◽  
Rong-Rong Zheng ◽  
Gui-Ling Fan ◽  
Jing-Hao Fan ◽  
Lin-Ping Zhao ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Photodynamic Therapy ◽  
Single Agent ◽  
Chimeric Peptide

Rotary movements and fluid membranes in termite flagellates

1976 ◽  
Vol 20 (3) ◽  
pp. 619-638
Author(s):  
S.L. Tamm ◽  
S. Tamm
Keyword(s):  
Plasma Membrane ◽  
Comparative Analysis ◽  
Cell Membrane ◽  
Shear Zone ◽  
Surrounding Medium ◽  
The Other ◽  
Visual Evidence ◽  
Fluid Membranes ◽  
Internal Motor ◽  
Rotary Motor

We previously described a remarkable type of cell motility that provided direct, visual evidence for the fluid nature of cell membranes. The movement involved continual, unidirectional rotation of one part of a protozoan, including the plasma membrane and cytoplasmic organelles, in relation to a neighbouring part. The cell membrane in the ‘shear zone’ appeared continuous with that of the rest of the cell. The rotary motor consisted, at least in part, of a non-contractile, microtubular axostyle which extended centrally through the cell. The protozoan was a devescovinid flagellate found in the hindgut of a Florida termite. In this paper, we have confirmed earlier reports of this type of motility in other kinds of devescovinids from Australian termites. By demonstrating continuity of the plasma membrane in the shear zone of the Australian devescovinids as well, we have obtained additional examples that provide direct, visual evidence for fluid membranes. A comparative analysis of rotational motility in various devescovinids revealed 2 different kinds of rotary mechanisms. Hyperdevescovina probably have an internal motor, in which one part of the cell exerts forces against another part, as in the Florida termite devescovinid. Devescovina species, on the other hand, have an external motor, in which flagellar and/or papillar movements exert forces against the surrounding medium. The structure of the axostyle in different devescovinids was compared, and its role in rotational motility discussed with respect to the behavioural data.

Goblet cell membrane differentiations in the midgut of a lepidopteran larva

1976 ◽  
Vol 20 (2) ◽  
pp. 357-375
Author(s):  
N.E. Flower ◽  
B.K. Filshie
Keyword(s):  
Plasma Membrane ◽  
Cell Membrane ◽  
Active Transport ◽  
Goblet Cell ◽  
Goblet Cells ◽  
Wide Channel ◽  
Lepidopteran Larvae ◽  
Lanthanum Tracer ◽  
Cell Cavity

So-called goblet cells are present in the midgut of lepidopteran larvae. They are thought to be involved in the active transport of potassium out of the haemolymph and into the gut lumen. A number of plasma membrane differentiations within the goblet cell cavity has been investigated using conventional staining, lanthanum tracer and freeze-etch techniques. Of particular interest are junction-like inter- and intra-membrane differentiations found on the villus-like cytoplasmic projections present at the apical tip of the goblet cell cavities. These cytoplasmic projections appear to act as a valve; in some cases they seem to close off the top of the goblet cell cavity, so isolating it from the gut lumen, while in other cases they are spread apart leaving a wide channel from the cavity into the lumen. The junction-like structures on these cytoplasmic projections are different in structure from the septate-type junctions which seal the midgut cells together at their apical borders, and the 2 types are present on the same plasma membrane, often within one micron of each other. The need for a different type of junction may possibly be related to the fact that it occurs between 2 areas of the same plasma membrane. The morphology of this unusual junction-like structure is discussed and 2 diagrams are presented to illustrate our interpretation of its structure.

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