scholarly journals An optimized purification process for porcine gastric mucin with preservation of its native functional properties

RSC Advances ◽  
2016 ◽  
Vol 6 (50) ◽  
pp. 44932-44943 ◽  
Author(s):  
Veronika J. Schömig ◽  
Benjamin T. Käsdorf ◽  
Christoph Scholz ◽  
Konstantinia Bidmon ◽  
Oliver Lieleg ◽  
...  
Keyword(s):  
Functional Properties ◽  
Gastric Mucin ◽  
Acidic Ph ◽  
Gel Formation ◽  
Purification Process ◽  
Porcine Gastric Mucin

The purification of porcine gastric mucin was optimized and key properties such as gel formation at acidic pH, lubrication behavior and interactions of mucins with charged molecules were preserved.

Correction to “Experimental and Theoretical Studies on the Binding of Epigallocatechin Gallate to Purified Porcine Gastric Mucin”

2012 ◽  
Vol 116 (43) ◽  
pp. 13149-13149
Author(s):  
Yanyan Zhao ◽  
Longjian Chen ◽  
Gleb Yakubov ◽  
Termeh Aminiafshar ◽  
Lujia Han ◽  
...  
Keyword(s):  
Epigallocatechin Gallate ◽  
Gastric Mucin ◽  
Theoretical Studies ◽  
Porcine Gastric Mucin ◽  
Experimental And Theoretical Studies

scholarly journals Biological Activity of Porcine Gastric Mucin on Stress Resistance and Immunomodulation

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 2981 ◽  
Author(s):  
Thiloma D. Liyanage ◽  
Pasan S. Dahanayake ◽  
Shan L. Edirisinghe ◽  
Chamilani Nikapitiya ◽  
Gang-Joon Heo ◽  
...  
Keyword(s):  
Heat Tolerance ◽  
Survival Rates ◽  
Gastric Mucin ◽  
Raw 264.7 Cells ◽  
Hatching Rate ◽  
Raw 264.7 ◽  
Ros Scavenging ◽  
Antioxidant Genes ◽  
Porcine Gastric Mucin ◽  
Wide Range

Purified porcine gastric mucin (PGM) is an alternative biomaterial to native mucin which displays multifunctional properties for exploring a wide range of biomedical applications. The present study evaluated the in vitro (RAW 264.7 macrophage cells) and in vivo (zebrafish embryos and larvae) bioactivities of PGM. The median lethal concentration (LC50) of PGM was 197.9 µg/mL for embryos, while it was non-toxic to RAW 264.7 cells, even at 500 µg/mL. Following PGM exposure (100 µg/mL), a higher embryo hatching rate (59.9%) was observed at 48 h post fertilization, compared to the control (30.6%). Protective effects of PGM from pathogenic Aeromonas hydrophila were demonstrated by high larvae survival rates of 85.0% and 94.0% at 50 and 100 μg/mL of PGM exposure, respectively. Heat tolerance effect of PGM (50 and 100 µg/mL) on larvae (40 °C for 48 h) was confirmed by 75% and 100% of survival rates, respectively. Additionally, PGM reduced the A. hydrophila–induced reactive oxygen species (ROS) generation in larvae. The qRT-PCR results in PGM exposed larvae exhibited induction of immune-related genes (tlr5a and tlr5b, myd88, c-rel, il1β, tnf-α, il6, il10, cxcl18b, ccl34a.4, defbl1, hamp, ctsd, muc2.1, muc5.1, muc5.2, and muc5.3), stress response (hsp70, hsp90aa1.1, and hsp90ab1), and antioxidant genes (cat and sod1). Moreover, our results revealed that PGM involved in the regulation of transcriptional gene induction increases Hsp90 protein in the zebrafish larvae. Furthermore, upregulation of Il6, Il10, Tnfα, Ccl3, Defa-rs2, Defa21 and Camp and antioxidant genes (Sod2 and Cat) were observed in PGM-exposed RAW 264.7 cells. Overall findings confirmed the activation of immune responses, disease resistance against pathogenic bacteria, heat tolerance, and ROS-scavenging properties by PGM, which may provide insights into new applications for PGM as a multifunctional immunomodulator.

scholarly journals Probing the Mucoadhesive Interactions Between Porcine Gastric Mucin and Some Water-Soluble Polymers

2015 ◽  
Vol 15 (11) ◽  
pp. 1546-1553 ◽  
Author(s):  
Yasser A. Albarkah ◽  
Rebecca J. Green ◽  
Vitaliy V. Khutoryanskiy
Keyword(s):  
Water Soluble ◽  
Gastric Mucin ◽  
Water Soluble Polymers ◽  
Soluble Polymers ◽  
Porcine Gastric Mucin

Fish pepsin: basic characteristics, extraction, determination and biotechnological applications

2015 ◽  
Vol 4 (1) ◽  
pp. 6-14
Author(s):  
Vagne De Melo Oliveira ◽  
Ranilson De Souza Bezerra ◽  
Caio Rodrigo Dias Assis
Keyword(s):  
Food Production ◽  
Specific Substrate ◽  
Acidic Ph ◽  
Purification Process ◽  
Biotechnological Applications ◽  
Basic Characteristics ◽  
Fish Viscera ◽  
Enzyme Targeting ◽  
Optimal Ph

Enzyme technology has been employed in various segments of the industry, food production and consumer goods. Fish viscera are potential sources of proteins with enzymatic properties appreciable in this market. Among them, pepsin (EC 3.4.23.x) deserves special attention. The active enzyme has at least five isoforms which are precursor groups of pepsins type: A, B, and F, gastricsin and chymosin. Their zymogen (pepsinogen) is activated by autocatalysis at acidic pH in stomach. Hemoglobin is their specific substrate. Features like optimal pH and temperature vary according to species and even within the same species may be found different values. The molecular weight also varies according to species and is generally between 25 and 35 kDa. The protocols for extraction and determination of pepsin fish follow a line of standardization, varying concentrations of analysis. These data are basic steps to detect the presence of the enzyme targeting a future purification process for its effective biotechnological applications.

Normal and Shear Forces between Surfaces Bearing Porcine Gastric Mucin, a High-Molecular-Weight Glycoprotein

2011 ◽  
Vol 12 (4) ◽  
pp. 1041-1050 ◽  
Author(s):  
Neale M. Harvey ◽  
Gleb E. Yakubov ◽  
Jason R. Stokes ◽  
Jacob Klein
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Gastric Mucin ◽  
Shear Forces ◽  
Porcine Gastric Mucin
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