Efficient and safe gene transfection in fish spermatogonial stem cells using nanomaterials

RSC Advances ◽  
2016 ◽  
Vol 6 (58) ◽  
pp. 52636-52641 ◽  
Author(s):  
F. M. P. Tonelli ◽  
S. M. S. N. Lacerda ◽  
N. C. O. Paiva ◽  
M. S. Lemos ◽  
A. C. de Jesus ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Death ◽  
Gene Delivery ◽  
Nile Tilapia ◽  
Gene Transfection ◽  
Spermatogonial Stem Cells ◽  
Efficient Gene

The nanomaterials MWCNTs, NGO and NRs can be functionalized and complexed to DNA to promote efficient gene delivery to Nile tilapia spermatogonial stem cells inducing less cell death than electroporation and the commercial reagents tested.

Gene delivery to Nile tilapia spermatogonial stem cells using carboxi-functionalized multiwall carbon nanotubes

RSC Advances ◽  
2014 ◽  
Vol 4 (72) ◽  
pp. 37985-37987 ◽  
Author(s):  
F. M. P. Tonelli ◽  
S. M. S. N. Lacerda ◽  
M. A. Silva ◽  
E. S. Ávila ◽  
L. O. Ladeira ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Stem Cells ◽  
Gene Delivery ◽  
Nile Tilapia ◽  
Transfection Efficiency ◽  
Multiwall Carbon Nanotubes ◽  
Spermatogonial Stem Cells ◽  
Cationic Lipids ◽  
Multiwall Carbon ◽  
Functionalized Multiwall Carbon Nanotubes

Carboxi-functionalized multiwall carbon nanotubes (fMWCNTs), when complexed with DNA, can promote gene delivery to Nile tilapia spermatogonial stem cells with higher transfection efficiency than cationic lipids or electroporation, causing also less cell death.

Efficient Gene Delivery to Mesenchymal Stem Cells by an Ethylenediamine-Modified Polysaccharide from Mulberry Leaves

Small ◽  
2011 ◽  
Vol 8 (3) ◽  
pp. 441-451 ◽  
Author(s):  
Wen-Wen Deng ◽  
Xia Cao ◽  
Miao Wang ◽  
Yan Yang ◽  
Wei-Yan Su ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Delivery ◽  
Mulberry Leaves ◽  
Efficient Gene

scholarly journals Ionic liquids with thioether motifs as synthetic cationic lipids for gene delivery

2017 ◽  
Vol 53 (59) ◽  
pp. 8328-8331 ◽  
Author(s):  
Jamie C. Gaitor ◽  
Lauren M. Paul ◽  
Melissa M. Reardon ◽  
Taha Hmissa ◽  
Samuel Minkowicz ◽  
...  
Keyword(s):  
Ionic Liquids ◽  
Gene Delivery ◽  
Click Chemistry ◽  
Gene Transfection ◽  
Cationic Lipids ◽  
Efficient Gene

Novel lipidic ionic liquids with imidazolium headgroups (red), thioether linkers (black), and two hydrophobic tails (blue) as efficient gene transfection vectors, synthesized via thiol–yne click chemistry.

Functionalized Scaffold for in Situ Efficient Gene Transfection of Mesenchymal Stem Cells Spheroids toward Chondrogenesis

2018 ◽  
Vol 10 (40) ◽  
pp. 33993-34004 ◽  
Author(s):  
Kunxi Zhang ◽  
Haowei Fang ◽  
Yechi Qin ◽  
Lili Zhang ◽  
Jingbo Yin
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Transfection ◽  
Efficient Gene

Dual-functionalized calcium carbonate based gene delivery system for efficient gene delivery

RSC Advances ◽  
2014 ◽  
Vol 4 (73) ◽  
pp. 38623-38629 ◽  
Author(s):  
Chao-Qun Wang ◽  
Meng-Qing Gong ◽  
Jin-Long Wu ◽  
Ren-Xi Zhuo ◽  
Si-Xue Cheng
Keyword(s):  
Calcium Carbonate ◽  
Gene Delivery ◽  
Delivery System ◽  
Gene Transfection ◽  
Cell Penetrating Peptide ◽  
Gene Delivery System ◽  
Dna Nanoparticles ◽  
Efficient Gene ◽  
Cell Penetrating ◽  
A Cell

Dual-functionalized KALA/PS/CaCO3/DNA nanoparticles containing a cell penetrating peptide (KALA) and protamine sulfate (PS) could effectively mediate gene transfection at a low DNA concentration.

scholarly journals Efficient gene delivery into undifferentiated stem cells and differentiated cardiomyocytes using lentiviral vectors

2003 ◽  
Vol 41 (6) ◽  
pp. 313
Author(s):  
Jin-Sook Kwon ◽  
Meizi Li ◽  
Young-Hwa Kim ◽  
Hainan Piao ◽  
Bo-Ra Son ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Lentiviral Vectors ◽  
Efficient Gene

Establishment of an electroporation-mediated gene delivery system in porcine spermatogonial stem cells

2019 ◽  
Vol 55 (3) ◽  
pp. 177-188
Author(s):  
Min Seong Kim ◽  
Min Hee Park ◽  
Ji Eun Park ◽  
Jung Im Yun ◽  
Jung Hoon Choi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Delivery System ◽  
Spermatogonial Stem Cells ◽  
Gene Delivery System

scholarly journals Erratum: TI - Efficient gene delivery in differentiated human embryonic stem cells

2006 ◽  
Vol 38 (5) ◽  
pp. 589-589
Author(s):  
Jae-Hwan Kim ◽  
Hyun-Jin Do ◽  
Seong-Jun Choi ◽  
Hyun-Jung Cho ◽  
Kyu-Hyung Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Delivery ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Efficient Gene
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