A 3D culture model study monitoring differentiation of dental epithelial cells into ameloblast-like cells

RSC Advances ◽  
2016 ◽  
Vol 6 (67) ◽  
pp. 62109-62118 ◽  
Author(s):  
Mayu Tadaki ◽  
Takahisa Anada ◽  
Yukari Shiwaku ◽  
Takashi Nakamura ◽  
Masanori Nakamura ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Three Dimensional ◽  
3D Culture ◽  
Epithelial Cell Line ◽  
Rat Incisor ◽  
Model Study ◽  
Culture Model ◽  
3D Culture Model ◽  
Study Monitoring

The present study was designed to investigate whether a three dimensional (3D) culture of the rat incisor-derived dental epithelial cell line SF2 enhances its differentiation into ameloblast-like cells.

scholarly journals Generation of a lung squamous cell carcinoma three-dimensional culture model with keratinizing structures

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shigeto Kawai ◽  
Kiyotaka Nakano ◽  
Keiichi Tamai ◽  
Etsuko Fujii ◽  
Mimori Yamada ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Notch Signaling ◽  
Squamous Epithelium ◽  
Three Dimensional ◽  
3D Culture ◽  
Lung Squamous Cell Carcinoma ◽  
Uniform Size ◽  
Secretase Inhibitor ◽  
Culture Model ◽  
3D Culture Model

AbstractTumor nests in lung squamous cell carcinoma (LUSC) have a hierarchical structure resembling squamous epithelium. The nests consist of basal-like cells on the periphery and layers of keratinocyte-like cells that differentiate towards the center of the nest, forming keratin pearls. Reproducing this spatial heterogeneity in in vitro models would be useful for understanding the biology of LUSC. Here, we established a three-dimensional (3D) culture model with a squamous epithelial structure using LUSC cell lines PLR327F-LD41 and MCC001F, established in-house. When PLR327F-LD41 cells were cultured in a mixture of Matrigel and collagen I, they generated 3D colonies (designated cancer organoids, or COs) with involucrin (IVL)-positive keratinizing cells in the center (IVLinner COs). COs with uniform size were generated by seeding PLR327F-LD41 cells in a form of small cell aggregates. Since Notch signaling induces the differentiation of squamous epithelium, we confirmed the effect of γ-secretase inhibitor in inhibiting Notch signaling in IVLinner COs. Surprisingly, γ-secretase inhibitor did not block induction of IVL-positive cells; however, cells residing between the CK5-positive basal-like layer and IVL-positive layer decreased significantly. Thus, our 3D culture model with uniform size and structure promises to be a useful tool for elucidating the biology of LUSC and for screening drug-candidates.

scholarly journals Study on a 3D Hydrogel-Based Culture Model for Characterizing Growth of Fibroblasts under Viral Infection and Drug Treatment

2017 ◽  
Vol 22 (5) ◽  
pp. 626-634 ◽  
Author(s):  
Xiaolu Zhu ◽  
Xianting Ding
Keyword(s):  
Morphological Evolution ◽  
Three Dimensional ◽  
3D Culture ◽  
Control Group ◽  
Herpes Simplex Virus 1 ◽  
3T3 Fibroblasts ◽  
Culture Model ◽  
3D Culture Model ◽  
Hsv 1

Three-dimensional (3D) in vitro tissue models provide an approach for the systematic, repetitive, and quantitative study of drugs. In this study, we constructed an in vitro 3D acrylated hyaluronic acid (AHA) hydrogel model encapsulating fibroblasts, performed long-period 3D culture, and tested cellular topological changes and proliferation variation in the presence of herpes simplex virus-1 (HSV-1) as an infecting virus and acyclovir (ACV) as the treatment drug. The AHA hydrogels were formed by using Michael addition chemistry of bis-cysteine containing MMP-degradable cross-linker onto AHA prefunctionalized with cell adhesion peptides (RGD). Cellular structures of 3T3 fibroblasts in hydrogel presented different morphological evolution processes and proliferation rates between different groups, including HSV-1 treated alone, ACV treated alone, HSV-1 and ACV cotreated, and control samples. In AHA hydrogel, ACV blocked HSV-1 infection/replication on fibroblasts. Yet, the proliferation of ACV-treated fibroblasts was slower than that of the control group. A significantly longer period was required for cells in 3D AHA gel to regain a healthy status when compared with cells in two-dimensional (2D) culture. This hydrogel-based 3D culture model potentially lays a foundation for analyzing the response of self-organized 3D tissues to viruses and drugs in a way that is closer to nature.

An Established Epithelial Cell Line (NPC/HK1) from a Nasopharyngeal Carcinoma - II. A Sem Study

1982 ◽  
Vol 40 ◽  
pp. 224-225
Author(s):  
Li C.L. ◽  
Chew E.C. ◽  
Huang D.P. ◽  
Ho H.C. ◽  
Mak L.S. ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epithelial Cell ◽  
Surface Morphology ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Present Communication ◽  
Cells In Culture ◽  
Sem Study ◽  
Well Differentiated

An epithelial cell line, NPC/HK1, has recently been successfully established from a nasopharyngeal carcinoma of the moderately to well differentiated squamous type. The present communication reports on the surface morphology of the NPC/HK1 cells in culture.

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