scholarly journals Label-free polymerization amplified potentiometric sensing platform for radical reactions using polyion sensitive membrane electrodes as transducers

RSC Advances ◽  
2016 ◽  
Vol 6 (42) ◽  
pp. 35628-35632
Author(s):  
Long Li ◽  
Guoliang Shang ◽  
Wei Qin
Keyword(s):  
Radical Polymerization ◽  
Radical Reactions ◽  
Membrane Electrodes ◽  
Label Free ◽  
Sensing Platform ◽  
Cascade Amplification ◽  
Sensitive Membrane

Based on the cascade amplification abilities of radical polymerization reactions, an amplified potentiometric sensing platform for radical reactions was developed.

scholarly journals Label-Free and Substrate-Free Potentiometric Aptasensing Using Polycation-Sensitive Membrane Electrodes

2012 ◽  
Vol 84 (4) ◽  
pp. 2055-2061 ◽  
Author(s):  
Jiawang Ding ◽  
Yan Chen ◽  
Xuewei Wang ◽  
Wei Qin
Keyword(s):  
Membrane Electrodes ◽  
Label Free ◽  
Sensitive Membrane

scholarly journals An Aptamer-Based Capacitive Sensing Platform for Specific Detection of Lung Carcinoma Cells in the Microfluidic Chip

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 98 ◽  
Author(s):  
Ngoc-Viet Nguyen ◽  
Chun-Hao Yang ◽  
Chung-Jung Liu ◽  
Chao-Hung Kuo ◽  
Deng-Chyang Wu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Lung Carcinoma ◽  
Early Stage ◽  
A549 Cells ◽  
Microfluidic Channel ◽  
Electrical Impedance Spectroscopy ◽  
Label Free ◽  
Early Stage Lung Cancer ◽  
Sensing Platform ◽  
Label Free Detection

Improvement of methods for reliable and early diagnosis of the cellular diseases is necessary. A biological selectivity probe, such as an aptamer, is one of the candidate recognition layers that can be used to detect important biomolecules. Lung cancer is currently a typical cause of cancer-related deaths. In this work, an electrical sensing platform is built based on amine-terminated aptamer modified-gold electrodes for the specific, label-free detection of a human lung carcinoma cell line (A549). The microdevice, that includes a coplanar electrodes configuration and a simple microfluidic channel on a glass substrate, is fabricated using standard photolithography and cast molding techniques. A procedure of self-assembly onto the gold surface is proposed. Optical microscope observations and electrical impedance spectroscopy measurements confirm that the fabricated microchip can specifically and effectively identify A549 cells. In the experiments, the capacitance element that is dominant in the change of the impedance is calculated at the appropriate frequency for evaluation of the sensitivity of the biosensor. Therefore, a simple, inexpensive, biocompatible, and selective biosensor that has the potential to detect early-stage lung cancer would be developed.

scholarly journals A label-free phosphorescence sensing platform for trypsin based on Mn-ZnS QDs

RSC Advances ◽  
2017 ◽  
Vol 7 (43) ◽  
pp. 26930-26934 ◽  
Author(s):  
Wei Liu ◽  
Huanhuan Li ◽  
Yanli Wei ◽  
Chuan Dong
Keyword(s):  
Label Free ◽  
Sensing Platform ◽  
Phosphorescence Probe

A label-free phosphorescence strategy to determine trypsin was proposed using Mn-ZnS QDs as the phosphorescence probe with a better validity.

Label-Free DNA Sensing Platform with Low-Voltage Electrolyte-Gated Transistors

2015 ◽  
Vol 87 (3) ◽  
pp. 1861-1866 ◽  
Author(s):  
Scott P. White ◽  
Kevin D. Dorfman ◽  
C. Daniel Frisbie
Keyword(s):  
Low Voltage ◽  
Label Free ◽  
Dna Sensing ◽  
Free Dna ◽  
Sensing Platform

scholarly journals Multitarget, quantitative nanoplasmonic electrical field-enhanced resonating device (NE2RD) for diagnostics

2015 ◽  
Vol 112 (32) ◽  
pp. E4354-E4363 ◽  
Author(s):  
Fatih Inci ◽  
Chiara Filippini ◽  
Murat Baday ◽  
Mehmet Ozgun Ozen ◽  
Semih Calamak ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Point Of Care ◽  
Medical Diagnostics ◽  
Clinical Samples ◽  
Label Free ◽  
Protein Biomarkers ◽  
Electrical Field ◽  
Color Changes ◽  
Sensing Platform ◽  
Broad Dynamic Range

Recent advances in biosensing technologies present great potential for medical diagnostics, thus improving clinical decisions. However, creating a label-free general sensing platform capable of detecting multiple biotargets in various clinical specimens over a wide dynamic range, without lengthy sample-processing steps, remains a considerable challenge. In practice, these barriers prevent broad applications in clinics and at patients’ homes. Here, we demonstrate the nanoplasmonic electrical field-enhanced resonating device (NE2RD), which addresses all these impediments on a single platform. The NE2RD employs an immunodetection assay to capture biotargets, and precisely measures spectral color changes by their wavelength and extinction intensity shifts in nanoparticles without prior sample labeling or preprocessing. We present through multiple examples, a label-free, quantitative, portable, multitarget platform by rapidly detecting various protein biomarkers, drugs, protein allergens, bacteria, eukaryotic cells, and distinct viruses. The linear dynamic range of NE2RD is five orders of magnitude broader than ELISA, with a sensitivity down to 400 fg/mL This range and sensitivity are achieved by self-assembling gold nanoparticles to generate hot spots on a 3D-oriented substrate for ultrasensitive measurements. We demonstrate that this precise platform handles multiple clinical samples such as whole blood, serum, and saliva without sample preprocessing under diverse conditions of temperature, pH, and ionic strength. The NE2RD’s broad dynamic range, detection limit, and portability integrated with a disposable fluidic chip have broad applications, potentially enabling the transition toward precision medicine at the point-of-care or primary care settings and at patients’ homes.

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