Enhanced long-term nitrogen removal by organotrophic anammox bacteria under different C/N ratio constraints: quantitative molecular mechanism and microbial community dynamics

RSC Advances ◽  
2016 ◽  
Vol 6 (90) ◽  
pp. 87593-87606 ◽  
Author(s):  
Duntao Shu ◽  
Yanling He ◽  
Hong Yue ◽  
Junling Gao ◽  
Qingyi Wang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Organic Carbon ◽  
Nitrogen Removal ◽  
Community Dynamics ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Microbial Community Dynamics ◽  
Low Levels ◽  
Anammox Process

The anaerobic ammonium oxidation (anammox) process has mainly been applied to NH4+–N-rich wastewater with very low levels of organic carbon (<0.5 g COD per g N).

scholarly journals Coupled anaerobic ammonium oxidation and hydrogenotrophic denitrification for simultaneous NH4-N and NO3-N removal

2018 ◽  
Vol 79 (5) ◽  
pp. 975-984 ◽  
Author(s):  
Tatsuru Kamei ◽  
Rawintra Eamrat ◽  
Kenta Shinoda ◽  
Yasuhiro Tanaka ◽  
Futaba Kazama
Keyword(s):  
Nitrogen Removal ◽  
Inorganic Nitrogen ◽  
Nitrate Removal ◽  
Fixed Bed ◽  
Anaerobic Ammonium Oxidation ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
N Removal ◽  
Anammox Process

Abstract Nitrate removal during anaerobic ammonium oxidation (anammox) treatment is a concern for optimization of the anammox process. This study demonstrated the applicability and long-term stability of the coupled anammox and hydrogenotrophic denitrification (CAHD) process as an alternative method for nitrate removal. Laboratory-scale fixed bed anammox reactors (FBR) supplied with H2 to support denitrification were operated under two types of synthetic water. The FBRs showed simultaneous NH4-N and NO3-N removal, indicating that the CAHD process can support NO3-N removal during the anammox process. Intermittent H2 supply (e.g. 5 mL/min for a 1-L reactor, 14/6-min on/off cycle) helped maintain the CAHD process without deteriorating its performance under long-term operation and resulted in a nitrogen removal rate of 0.21 kg-N/m3/d and ammonium, nitrate, and dissolved inorganic nitrogen removal efficiencies of 73.4%, 80.4%, and 77%, respectively. The microbial community structure related to the CAHD process was not influenced by changes in influent water quality, and included the anammox bacteria ‘Candidatus Jettenia’ and a Sulfuritalea hydrogenivorans-like species as the dominant bacteria even after long-term reactor operation, suggesting that these bacteria are key to the CAHD process. These results indicate that the CAHD process is a promising method for enhancing the efficiency of anammox process.

Nitrogen removal from food waste digestate using partial nitritation-anammox process: Effect of different aeration strategies on performance and microbial community dynamics

2019 ◽  
Vol 251 ◽  
pp. 109562 ◽  
Author(s):  
Alyne Duarte Pereira ◽  
Luyara de Almeida Fernandes ◽  
Helena Maria Campos Castro ◽  
Cíntia Dutra Leal ◽  
Brenda Gonçalves Piteira Carvalho ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nitrogen Removal ◽  
Food Waste ◽  
Community Dynamics ◽  
Partial Nitritation ◽  
Microbial Community Dynamics ◽  
Anammox Process

scholarly journals Soil microbial community dynamics and assembly under long-term land use change

2017 ◽  
Vol 93 (10) ◽  
Author(s):  
Dennis Goss-Souza ◽  
Lucas William Mendes ◽  
Clovis Daniel Borges ◽  
Dilmar Baretta ◽  
Siu Mui Tsai ◽  
...  
Keyword(s):  
Land Use ◽  
Microbial Community ◽  
Land Use Change ◽  
Soil Microbial Community ◽  
Community Dynamics ◽  
Soil Microbial ◽  
Microbial Community Dynamics

scholarly journals Long‐term microbial community dynamics at two full‐scale biotrickling filters treating pig house exhaust air

2019 ◽  
Vol 12 (4) ◽  
pp. 775-786 ◽  
Author(s):  
Caroline Van der Heyden ◽  
Thijs De Mulder ◽  
Eveline I. P. Volcke ◽  
Peter Demeyer ◽  
Marc Heyndrickx ◽  
...  
Keyword(s):  
Microbial Community ◽  
Community Dynamics ◽  
Full Scale ◽  
Biotrickling Filters ◽  
Microbial Community Dynamics

scholarly journals Achieving fast start-up of anammox process by promoting the growth of anammox bacteria with FeS addition

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Chunzhen Zou ◽  
Beibei Guo ◽  
Xuming Zhuang ◽  
Liying Ren ◽  
Shou-Qing Ni ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nitrogen Removal ◽  
Nitrite Reductase ◽  
Removal Rate ◽  
Anammox Bacteria ◽  
Gene Encoding ◽  
Start Up ◽  
Fast Start ◽  
The Difference ◽  
Anammox Process

Abstract The effects of FeS on nitrogen removal performance and microbial community of anammox process were studied. During the start-up period, the removal efficiencies of nitrite and total nitrogen were significantly improved by FeS. The addition of FeS increased the content of iron ions in the reactor and promoted the synthesis of heme c, which was involved in the formation of various enzymes. Compared with the control, the abundance of anammox bacteria in the FeS reactor was increased by 29%, and the expression level of the nirS gene (encoding cd1 type nitrite reductase containing heme) was nearly doubled. The content of nitrite reductase (ammonia-forming) in the community was increased by 26.4%. The difference in functional bacteria and enzyme contents in the microbial community resulted in a difference in nitrogen removal rate (NRR) between the two reactors. High-throughput results indicated that FeS increased the richness and diversity of microbial community and enhanced the metabolic function of the microbial community. The addition of FeS did not change the dominant position of Ca. Kuenenia in both reactors. But the relative abundance of heterotrophic denitrifying bacteria was reduced with FeS, which may be related to the inhibition effect of S2− produced by FeS.

scholarly journals Batch enrichment of anammox bacteria and study of the underlying microbial community dynamics

2016 ◽  
Vol 297 ◽  
pp. 217-228 ◽  
Author(s):  
Romain Connan ◽  
Patrick Dabert ◽  
Hassan Khalil ◽  
Gilbert Bridoux ◽  
Fabrice Béline ◽  
...  
Keyword(s):  
Microbial Community ◽  
Community Dynamics ◽  
Anammox Bacteria ◽  
Microbial Community Dynamics

scholarly journals Cometabolism of the Superphylum Patescibacteria with Anammox Bacteria in a Long-Term Freshwater Anammox Column Reactor

Water ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 208
Author(s):  
Suguru Hosokawa ◽  
Kyohei Kuroda ◽  
Takashi Narihiro ◽  
Yoshiteru Aoi ◽  
Noriatsu Ozaki ◽  
...  
Keyword(s):  
Heterotrophic Bacteria ◽  
Draft Genome ◽  
Marker Genes ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Column Reactor ◽  
Nitrite Nitrate ◽  
Anammox Process ◽  
Candidate Phyla Radiation

Although the anaerobic ammonium oxidation (anammox) process has attracted attention regarding its application in ammonia wastewater treatment based on its efficiency, the physiological characteristics of anammox bacteria remain unclear because of the lack of pure-culture representatives. The coexistence of heterotrophic bacteria has often been observed in anammox reactors, even in those fed with synthetic inorganic nutrient medium. In this study, we recovered 37 draft genome bins from a long-term-operated anammox column reactor and predicted the metabolic pathway of coexisting bacteria, especially Patescibacteria (also known as Candidate phyla radiation). Genes related to the nitrogen cycle were not detected in Patescibacterial bins, whereas nitrite, nitrate, and nitrous oxide-related genes were identified in most of the other bacteria. The pathway predicted for Patescibacteria suggests the lack of nitrogen marker genes and its ability to utilize poly-N-acetylglucosamine produced by dominant anammox bacteria. Coexisting Patescibacteria may play an ecological role in providing lactate and formate to other coexisting bacteria, supporting growth in the anammox reactor. Patescibacteria-centric coexisting bacteria, which produce anammox substrates and scavenge organic compounds produced within the anammox reactor, might be essential for the anammox ecosystem.

scholarly journals Start-Up of Anammox SBR from Non-Specific Inoculum and Process Acceleration Methods by Hydrazine

Water ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 350
Author(s):  
Ivar Zekker ◽  
Oleg Artemchuk ◽  
Ergo Rikmann ◽  
Kelvin Ohimai ◽  
Gourav Dhar Bhowmick ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Biological Nutrient Removal ◽  
Anaerobic Sludge ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Reject Water ◽  
Start Up ◽  
Anammox Activity ◽  
Optimum Salinity ◽  
Anammox Process

Biological nutrient removal from wastewater to reach acceptable levels is needed to protect water resources and avoid eutrophication. The start-up of an anaerobic ammonium oxidation (anammox) process from scratch was investigated in a 20 L sequence batch reactor (SBR) inoculated with a mixture of aerobic and anaerobic sludge at 30 ± 0.5 °C with a hydraulic retention time (HRT) of 2–3 days. The use of NH4Cl, NaNO2, and reject water as nitrogen sources created different salinity periods, in which the anammox process performance was assessed: low (<0.2 g of Cl−/L), high (18.2 g of Cl−/L), or optimum salinity (0.5–2 g of Cl−/L). Reject water feeding gave the optimum salinity, with an average nitrogen removal efficiency of 80%, and a TNRR of 0.08 kg N/m3/d being achieved after 193 days. The main aim was to show the effect of a hydrazine addition on the specific anammox activity (SAA) and denitrification activity in the start-up process to boost the autotrophic nitrogen removal from scratch. The effect of the anammox intermediate hydrazine addition was tested to assess its concentration effect (range of 2–12.5 mg of N2H4/L) on diminishing denitrifier activity and accelerating anammox activity at the same time. Heterotrophic denitrifiers’ activity was diminished by all hydrazine additions compared to the control; 5 mg of N2H4/L added enhanced SAA compared to the control, achieving an SAA of 0.72 (±0.01) mg N/g MLSS/h, while the test with 7.5 mg of N2H4/L reached the highest overall SAA of 0.98 (±0.09) mg N g/MLSS/h. The addition of trace amounts of hydrazine for 6 h was also able to enhance SAA after inhibition by organic carbon source sodium acetate addition at a high C/N ratio of 10/1. The start-up of anammox bacteria from the aerobic–anaerobic suspended biomass was successful, with hydrazine significantly accelerating anammox activity and decreasing denitrifier activity, making the method applicable for side-stream as well as mainstream treatment.

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