Exploring the interaction of l-cysteine capped CuS nanoparticles with bovine serum albumin (BSA): a spectroscopic study

RSC Advances ◽  
2016 ◽  
Vol 6 (63) ◽  
pp. 58288-58295 ◽  
Author(s):  
S. Prasanth ◽  
D. Rithesh Raj ◽  
T. V. Vineeshkumar ◽  
Riju K. Thomas ◽  
C. Sudarsanakumar
Keyword(s):  
Particle Size ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Copper Sulfide ◽  
Average Particle Size ◽  
Precipitation Method ◽  
Average Particle ◽  
Spectroscopic Techniques ◽  
Co Precipitation

l-Cysteine capped copper sulfide nanoparticles with an average particle size of 6 nm were synthesized using a chemical co-precipitation method and their interactions with bovine serum albumin were explored using various spectroscopic techniques.

Box-Behnken Design Optimized TPGS Coated Bovine Serum Albumin Nanoparticles Loaded with Anastrozole

2021 ◽  
Vol 18 ◽  
Author(s):  
Ashish Kumar ◽  
Ajit Singh ◽  
S.J.S Flora ◽  
Rahul Shukla
Keyword(s):  
Particle Size ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Glycol Succinate ◽  
Burst Release ◽  
Spectroscopic Techniques ◽  
Potential Candidate ◽  
Box Behnken Design ◽  
Thermal Gelation

Purpose: In this study, a novel D-α-tocopheryl polyethylene glycol succinate (TPGS) modified bovine serum albumin (BSA) nanoparticles were developed for delivery of Anastrozole (ANZ) which is optimized by Box-Behnken design (BBD). This TPGS-ANZ-BSA NPs are evaluated for their physicochemical and drug release characteristics. Methods: TPGS-ANZ-BSA NPs were prepared by desolvation thermal gelation method andthe effects of critical process parameter (CPP)which are BSA amount, TPGS concentration and stirring speed on the critical quality attributes (CQA) such as % drug loading (%DL) and particle size were studied using BBD. TPGS-ANZ-BSA NPs were characterized using different spectroscopic techniques including UV-Visible and FTIR is used to confirm the entrapment of ANZ in BSA. DSC and PXRD revealed the amorphization of ANZ in the TPGS-ANZ-BSA NPs after freeze drying. Scanning electron microscopy (SEM) analysis was performed for the surface morphologyanalysesNPs. In vitro release studies were performed at pH 5.5 and pH 7.4 for 48h to mimic tumour microenvironment. Results: The BBD optimized batch showed 107 nm particle size with % DL of 8.5± 0.5 of TPGS-ANZ-BSA NPs. The spectroscopic and thermal characterizations revealed the successful encapsulation of ANZ inside the nanoparticles.The TPGS-ANZ-BSA NPs were found to exhibit burst release at pH 5.5 and sustained release at pH 7.4. The short-term stability of drug-loaded nanoparticles displayed no significant changes in physicochemical properties at room temperature for period of one month. Conclusion: The BBD optimized TPGS-ANZ-BSA nanoparticles showed enhanced physiochemical properties for ANZ and potential candidate for anticancer agent drugs delivery.

Morphology-Controlled CaCO3 Nanostructures by Modified Co-Precipitation in Pulsed Mode

2015 ◽  
Vol 752-753 ◽  
pp. 148-153
Author(s):  
M.M. Nassar ◽  
Taha Ebrahiem Farrag ◽  
M.S. Mahmoud ◽  
Sayed Abdelmonem
Keyword(s):  
Particle Size ◽  
Calcium Carbonate ◽  
Rotation Speed ◽  
Average Particle Size ◽  
Calcium Nitrate ◽  
Precipitation Method ◽  
Average Particle ◽  
X Ray ◽  
Co Precipitation ◽  
Pulsed Mixing

Calcium carbonate nanoparticles and nanorods were synthesized by precipitation from saturated sodium carbonate and calcium nitrate aqueous solutions through co precipitation method. A new rout of synthesis was done by both using pulsed mixing method and controlling the addition of calcium nitrate. The effect of the agitation speed, and the temperature on particle size and morphology were investigated. Particles were characterized using X-ray Microanalysis, X-ray analysis (XRD) and scanning electron microscopy (SEM). The results indicated that increasing the mixer rotation speed from 3425 to 15900 (rpm) decreases the average particle size to 64±7 nm. A rapid nucleation then aggregation induced by excessive shear force phenomena could explain this observation. Moreover, by increasing the reaction temperature, the products were converted from nanoparticle to nanorods. The maximum attainable aspect ratio was 6.23 at temperature of 75°C and rotation speed of 3425. Generally, temperature raise promoted a significant homoepitaxial growth in one direction toward the formation of calcite nanorods. Overall, this study can open new avenues to control the morphology of the calcium carbonate nanostructures.

Effect of Surfactant on the Preparation of Nano-powder for Yb Doping Laser Transparent YAG Ceramic

2013 ◽  
Vol 32 (5) ◽  
pp. 511-515 ◽  
Author(s):  
Xiao Guo Cao ◽  
Jia Wang ◽  
Qi Bai Wu ◽  
Hai Yan Zhang
Keyword(s):  
Particle Size ◽  
Polyethylene Glycol ◽  
Average Particle Size ◽  
Ammonium Bicarbonate ◽  
Spherical Particles ◽  
Precipitation Method ◽  
Average Particle ◽  
X Ray Diffraction ◽  
Nano Powder ◽  
Co Precipitation

AbstractYb:YAG transparent ceramic nano-powder was prepared by chemical co-precipitation method, with ammonium bicarbonate as the precipitant and polyethylene glycol as surfactant. The addition of polyethylene glycol can reduce the agglomeration and particle size of the prepared Yb:YAG powder. The morphology, thermal stability and phase structure of Yb:YAG nano-powder were charactered by scanning electron microscopy (SEM), thermogravimetry and differential thermal analysis (TG-DTA), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy. The results show that well-crystallized nano-powder was obtained by calcining the precursors at 900 °C for 3 h. The average particle size of Yb:YAG powder is about 100–200 nm. When the volume amount of polyethylene glycol is 2.0%, well-dispersed Yb:YAG powder with spherical particles of 100 nm diameter was obtained.

Preparation of Nanostructured Metal Ferrite Particles by Sonochemistry

2005 ◽  
Vol 277-279 ◽  
pp. 1044-1048 ◽  
Author(s):  
Eun Hee Kim ◽  
Hyo Sook Lee ◽  
Hui Ping Shao
Keyword(s):  
Magnetic Field ◽  
Particle Size ◽  
Cobalt Ferrite ◽  
Average Particle Size ◽  
Iron Chloride ◽  
Precipitation Method ◽  
Average Particle ◽  
Sonochemical Method ◽  
Iron Ferrite ◽  
Co Precipitation

Nanostructured iron and cobalt ferrite particles were prepared from iron chloride and cobalt chloride, respectively, using the sonochemical method. The particles were compared with those synthesized using the co-precipitation method. The properties of the particles were characterized using various techniques, such as XRD, TEM, VSM and a SQUID magnetometer. The iron ferrite particles had an average particle size of about 15 nm and a magnetization value of 83 emu/g at a magnetic field of 50 kOe, while the particle size of cobalt ferrite was about 5 nm and its magnetization value was 33 emu/g at the same magnetic field.

scholarly journals Crystal structure, optical and magnetic properties of PrFeO3 nanoparticles prepared by modified co-precipitation method

2020 ◽  
Vol 14 (4) ◽  
pp. 355-361
Author(s):  
Anh Nguyen ◽  
Ngoc Nguyen ◽  
Irina Mittova ◽  
Nikolai Perov ◽  
Valentina Mittova ◽  
...  
Keyword(s):  
Particle Size ◽  
Single Phase ◽  
Average Particle Size ◽  
Precipitation Method ◽  
Average Particle ◽  
Strong Adsorption ◽  
Different Temperatures ◽  
Paramagnetic Materials ◽  
Co Precipitation ◽  
Potential Use

In this work, PrFeO3 nanoparticles were synthesized by modified co-precipitation method and annealed at different temperatures up to 850?C. The annealed PrFeO3 nanoparticles have single phase orthorhombic structure and the average particle size of 25-30 nm. Due to the very small particle size the prepared PrFeO3 nanoparticles are capable of being used as photocatalyst materials thanks to their strong adsorption bands at 230-400 nm and 400-800 nm observed from the UV-Vis spectra. Additionally, the PrFeO3 nanoparticles are paramagnetic materials with Hc ~ 10Oe and Mr ~ 0. These findings demonstrate their potential use not only as photocatalysts, but also as magnetic materials.

Synthesis and Studying Induction Heating of Mn1-xZnxFe2O4 (x=0-0.5) Magnetic Nanoparticles for Hyperthermia Treatments

2021 ◽  
Vol 882 ◽  
pp. 200-218
Author(s):  
S. Mahmood Hussein ◽  
T.H. Mubarak ◽  
S.M. Ali Ridha ◽  
Jasim Al-Zanganawee
Keyword(s):  
Particle Size ◽  
Relaxation Time ◽  
Magnetic Nanoparticles ◽  
Smart Materials ◽  
Average Particle Size ◽  
Superparamagnetic Nanoparticles ◽  
Soft Magnetic Materials ◽  
Precipitation Method ◽  
Average Particle ◽  
Co Precipitation

The recent development of the using the magnetic nanoparticles for hyperthermia treatments emphasizes the needed of smart materials to become a safety for heat therapy. Self-regulate magnetic nanoparticles of MnZnFe2O4 may be proper for thermal treatments. Structure and magnetic properties of the synthesis Mn1-xZnx Fe2O4 with x=0- 0.5 by step 0.1were studied. Superparamagnetic nanoparticles of MnZnFe2O4 were prepared by co-precipitation method, followed that heat treatment in the autoclave reactor. XRD results showed that is difficult to prepare MnZnFe2O4 directly using the co-precipitation method. Preparation method yield nanoparticles with spherical shape and there is a slight change in the particle size distribution, also observed shrinkage occurs in the particle size after heat treatments, the average particle size was estimated about 20nm as confirmed by FESEM images. FTIR spectra of samples showed two distinct absorption peaks in the range ~ 617 – 426 (cm-1) related to stretching vibrations of the (Fe-O) in the tetrahedral and octahedral side respectively. Magnetic measurements were carried out using (VSM), M-H curves indicate typical soft magnetic materials and particles so small to be identical superparamagnetic nanoparticles. Heating ability of water based colloidal dispersions of samples were studied under magnetic field strength 6.5kA/m and the frequency 190 kHz, and the results showed when increasing Zn2+ to x=0.3 or more the samples not heated up. Depending on the heating curve susceptibility, effective relaxation time and Néel relaxation time , were determined.

scholarly journals Superior Performance of Magnetic Nanoparticles for Entrapment and Fixation of Bovine Serum Albumin In-Vitro

2020 ◽  
Vol 30 (4) ◽  
Author(s):  
Mansour Binandeh ◽  
Farrokh Karimi ◽  
Sadegh Rostamnia
Keyword(s):  
Magnetic Nanoparticles ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Superior Performance ◽  
Precipitation Method ◽  
Experimental Conditions ◽  
Ft Ir ◽  
Co Precipitation

BACKGROUND: In recent years, extensive studies have been performed on magnetite nanoparticles (MNPs) and their applications, which have shown the current project to be one of the major applications by laboratory results.METHODS: The nanoparticles synthesized in this project were deposited by the co-precipitation method, which structure was identified by analyzers such as SEM, FT-IR, and EDX. The aim of this project is the adsorption and fixation of biomolecule (BSA (bovine serum albumin) protein on the surface of magnetic nanoparticles.RESULTS: The adsorption results by electrophoresis and spectrophotometric analyzers showed an absorption rate above 55% ie; 55% of the protein is fixed on the MNPs nanoparticles. This absorption is due to the high level of functionality of magnetic nanoparticles for adsorption of protein. The results of the EDX analysis also show the possible electrostatic bonding between the nanoparticles and the protein, this is derived from –OH with –NH2 groups of the nanobiocompound (MNPs /protein). After bonding, the two are easily separated.CONCLUSION: In this project, the Fe3O4 nanoparticles was synthesized and identified by SEM, FT-IR, and EDX analyzers and finally reacted with the BSA protein (for the absorption of protein on MNPs) under experimental conditions at a standard temperature of 25° C. The results showed that about 55% of the protein was fixed on magnetic nanoparticles.

scholarly journals SYNTHESIS OF CdS NANOPARTICLES BY CHEMICAL Co-PRECIPITATION METHOD AND ITS COMPARATIVE ANALYSIS OF PARTICLE SIZE VIA STRUCTURAL AND OPTICAL CHARACTERIZATION

2020 ◽  
Vol 3 (3) ◽  
pp. 100
Author(s):  
Syed Kaabir Ali ◽  
Henna Wani ◽  
Chandramani Upadhyay ◽  
K.S.S.N. Sai Madhur ◽  
Imran Khan ◽  
...  
Keyword(s):  
Particle Size ◽  
Lattice Strain ◽  
Theoretical Calculations ◽  
Average Particle Size ◽  
Precipitation Method ◽  
Cds Nanoparticles ◽  
Deformation Model ◽  
Average Particle ◽  
Scherrer Equation ◽  
Co Precipitation

CdS is an important wide bandgap chalcogenides most popularly studied for various optoelectronics and biosensing applications. In this study, CdS Nanoparticles (NPs) have been prepared successfully by chemical co-precipitation method, using cadmium acetate and sodium sulphide as precursors. A comparative study of average particle size calculated by Scherrer Plot, Uniform Deformation Model (UDM), Dynamic Light Scattering (DLS) analysis and Brus Model has been done here. The structural and optical behaviour of synthesized samples were investigated via X-ray diffraction (XRD), DLS and UV–Visible Spectroscopy. The XRD spectra of the prepared CdS NPs revealed the crystalline phase having cubic structure. The average particles size has been studied via Debye Scherrer equation and Scherrer Plot. For the theoretical calculations of particle size along with the induced lattice strain, considering the broadening effect of lattice strain, Williamson-Hall analysis was employed. Assuming the lattice strain to be isotropic in nature, UDM was applied for calculation. The particles size distribution profile in terms of volume as well as intensity was recorded using DLS analysis in ethanol medium at room temperature. Besides this, the energy bandgap was obtained by applying Tauc model in the recorded absorption spectra. The obtained value of bandgap was used in Brus model for estimating the average particle size. The obtained comparative results show that the average particle size of the prepared CdS NPs estimated from Scherrer equation, Scherrer plot, UDM plot and Brus model are almost similar and lies in the range of 2-5 nm whereas the results of DLS showed wide variation in the range of 40-600 nm.

scholarly journals STUDI PELEPASAN TERKONTROL TERHADAP NANOENKAPSULASI DIMETOKSI AMINO CALKON SEBAGAI DESAIN KANDIDAT SENYAWA ANTI KANKER YANG EFEKTIF

2016 ◽  
Vol 1 (2) ◽  
pp. 94
Author(s):  
Mochamamad Zakki Fahmi ◽  
Hery Suwito ◽  
Shofi Yasmin Nurain ◽  
Yogi Putra Hidayatullah
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Average Particle Size ◽  
Average Particle ◽  
Solubility In Water ◽  
Salt Addition ◽  
Anti Cancer ◽  
Low Solubility ◽  
Anticancer Compound

ABSTRAKEnkapsulasi merupakan sebuah proses dimana partikel kecil dikemas dalam sebuah partikel yang lebih besar sehingga membentuk kapsul. Metode tersebut akan digunakan untuk memodifikasi calkon, senyawa anti kanker yang memiliki kelarutan dalam air sangat rendah, dengan menggunakan Bovine Serum Albumin sebagai enkapsulan. Modifikasi senyawa calkon ini dilakukan dengan mencampurkan larutan BSA dalam air dan larutan calkon dalam kloroform kemudian diultrasonikasi. Senyawa hasil sonikasi tersebut disebut produk nanoenkapsulan BSA-calkon. Produk selanjutnya diuji ketahanannya terhadap perubahan pH, penambahan garam dan suhu. Produk kemudian dikarakterisasi menggunakan spektrofotometer UV-Vis, FTIR dan DLS. Proses nanoenkapsulasi dapat dikatakan berhasil dilakukan, ditunjukkan dengan produk nanoenkapsulan BSA-calkon yang dapat larut dalam air. Hasil karakterisasi menggunakan DLS menunjukkan bahwa produk nanoenkapsulan BSA-amino calkon memiliki rata-rata diameter partikel sebesar 457,5 nm dan 201,0 nm untuk produk nanoenkapsulan BSA-dimetoksi amino calkon. Hasil FTIR dari nanoenkapsulan BSA-amino calkon memunculkan serapan gugus amida pada 1639,55 cm-1. Sedangkan pada nanoenkapsulan BSA-dimetoksi amino calkon, gugus amida muncul pada serapan 1635,69 cm-1.Kata kunci : Nanoenkapsulasi, calkon, Bovine Serum Albumin, anti kanker.ABSTRACTEncapsulation is a process where a small particles packaged in a larger particles and it forms into a capsule. This method will be used to modify chalcone, an anticancer compound that have very low solubility in water. So it can’t be applied into human bodies. This chalcone will be encapsulated by Bovine Serum Albumin. Modification of chalcone is carried out by mixing a BSA solution in water and chalcone solution in chloroform by an ultrasonication process. The product will be tested for the resistance of pH, salt addition and temperature. The products also characterized using UV-Vis, FTIR and DLS instruments. Nanoencapsulation process was successfully do, it’s indicated by the nanoencapsulan product that has a high solubility in water. The results of DLS indicate that products have an average particle size is 457,5 nm for BSA-amine chalcone and 201,0 nm for BSA-dimethoxy amine chalcone. FTIR results shows that nanoencapsulation BSA-chalcone has amide groups, it showed by the absorption peak which raises at 1639,55 cm-1 for amine chalcone, and 1635,69 cm-1 for dimethoxy amine chalcone.Keywords : Nanoencapsulation, chalcone, bovine serum albumin, anti-cancer.

Chemical Synthesis and Characterization of Bismuth Vanadate Powder

2010 ◽  
Vol 93-94 ◽  
pp. 153-156 ◽  
Author(s):  
Pusit Pookmanee ◽  
Sumintra Paosorn ◽  
Sukon Phanichphant
Keyword(s):  
Particle Size ◽  
Single Phase ◽  
Average Particle Size ◽  
Ammonium Hydroxide ◽  
Bismuth Vanadate ◽  
Precipitation Method ◽  
Average Particle ◽  
X Ray Diffraction ◽  
Co Precipitation

Bismuth vanadate powder was synthesized by a chemical co-precipitation method. Bismuth nitrate and ammonium vanadate were used as the starting precursors. The yellow precipitated powder was formed after adding ammonium hydroxide until the pH of final solution was 7. The powder was filtered and dried at 60 °C for 24h and calcined at 200-400 °C for 2h. The phase of bismuth vanadate powder was studied by X-ray diffraction (XRD). A single phase of monoclinic structure was obtained after calcinations at 200-400 °C for 2h. The morphology and particle size of bismuth vanadate powder were investigated by scanning electron microscopy (SEM). The particle was irregular in shape and highly agglomerated with an average particle size of 0.5 µm in width and 1.5 µm in length.

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