Synthesis of a glucose oxidase-conjugated, polyacrylamide-based, fluorescent hydrogel for a reusable, ratiometric glucose sensor

2016 ◽  
Vol 7 (43) ◽  
pp. 6655-6661 ◽  
Author(s):  
Yongkyun Kim ◽  
Ho Namgung ◽  
Taek Seung Lee
Keyword(s):  
Glucose Oxidase ◽  
Glucose Sensor ◽  
Glucose Sensing ◽  
Emission Color

New emission color-changeable hydrogels containing glucose oxidase were synthesized to be used in glucose sensing.

scholarly journals Reduced graphene oxide-supported methylene blue nanocomposite as a glucose oxidase-mimetic for electrochemical glucose sensing

RSC Advances ◽  
2018 ◽  
Vol 8 (57) ◽  
pp. 32565-32573 ◽  
Author(s):  
Shaojun Yang ◽  
Daliang Liu ◽  
Qing Bo Meng ◽  
Shuyao Wu ◽  
Xi-Ming Song
Keyword(s):  
Methylene Blue ◽  
Graphene Oxide ◽  
Glucose Oxidase ◽  
Reduced Graphene Oxide ◽  
Electrocatalytic Activity ◽  
Glucose Sensor ◽  
Glucose Sensing ◽  
Reduced Graphene

A novel electrochemical glucose sensor based on methylene blue-reduced graphene oxide nanocomposite was constructed, and the sensor exhibited good glucose oxidase-mimetic electrocatalytic activity towards glucose and practical applicability.

scholarly journals Glucose Sensor Using Redox Active Oligonucleotide-Templated Silver Nanoclusters

Nanomaterials ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 1065 ◽  
Author(s):  
Kathryn L. Schroeder ◽  
Renee V. Goreham ◽  
Thomas Nann
Keyword(s):  
Glucose Oxidase ◽  
Glucose Sensor ◽  
Glucose Sensing ◽  
Emission Peak ◽  
Silver Nanoclusters ◽  
Glucose Levels ◽  
Redox Active ◽  
Emission Change

Redox active, photoluminescent silver nanoclusters templated with oligonucleotides were developed for glucose sensing. The silver nanoclusters had a photoluminescent emission at 610 nm that reversibly changed to 530 nm upon oxidation. The reversible emission change was measured with photoluminescent spectroscopy and used to detect H2O2, which is a by-product of the reaction of glucose with glucose oxidase. The ratio of the un-oxidised emission peak (610 nm) and the oxidised analogue (530 nm) was used to measure glucose concentrations up to 20 mM, well within glucose levels found in blood. Also, the reversibility of this system enables the silver nanoclusters to be reused.

scholarly journals Development of a Ratiometric Fluorescent Glucose Sensor Using an Oxygen-Sensing Membrane Immobilized with Glucose Oxidase for the Detection of Glucose in Tears

Biosensors ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 86 ◽  
Author(s):  
Hong Dinh Duong ◽  
Ok-Jae Sohn ◽  
Jong Il Rhee
Keyword(s):  
Glucose Oxidase ◽  
Glucose Concentration ◽  
Glucose Sensor ◽  
Oxygen Sensing ◽  
Sol Gel ◽  
Glucose Sensing ◽  
Glucose Biosensor ◽  
Detection Range ◽  
Fluorescence Measurements ◽  
Sensing Membrane

Glucose concentration is an important parameter in biomedicine since glucose is involved in many metabolic pathways in organisms. Many methods for glucose detection have been developed for use in various applications, particularly in the field of healthcare in diabetics. In this study, ratiometric fluorescent glucose-sensing membranes were fabricated based on the oxygen levels consumed in the glucose oxidation reaction under the catalysis of glucose oxidase (GOD). The oxygen concentration was measured through the fluorescence quenching effect of an oxygen-sensitive fluorescent dye like platinum meso-tetra (pentafluorophenyl) porphyrin (PtP) by oxygen molecules. Coumarin 6 (C6) was used as a reference dye in the ratiometric fluorescence measurements. The glucose-sensing membrane consisted of two layers: The first layer was the oxygen-sensing membrane containing polystyrene particles (PS) doped with PtP and C6 (e.g., PS@C6^PtP) in a sol–gel matrix of aminopropyltrimethoxysilane and glycidoxypropyltrimethoxysilane (GA). The second layer was made by immobilizing GOD onto one of three supporting polymers over the first layer. These glucose-sensing membranes were characterized in terms of their response, reversibility, interferences, and stability. They showed a wide detection range to glucose concentration in the range of 0.1 to 10 mM, but high sensitivity with a linear detection range of 0.1 to 2 mM glucose. This stable and sensitive ratiometric fluorescent glucose biosensor provides a reliable way to determine low glucose concentrations in blood serum by measuring tear glucose.

scholarly journals Waste eggshell membrane-templated synthesis of functional Cu2+–Cu+/biochar for an ultrasensitive electrochemical enzyme-free glucose sensor

RSC Advances ◽  
2021 ◽  
Vol 11 (31) ◽  
pp. 18994-18999
Author(s):  
Linzhi Li ◽  
Tianzeng Huang ◽  
Saijun He ◽  
Xing Liu ◽  
Qi Chen ◽  
...  
Keyword(s):  
Glucose Sensor ◽  
Glucose Sensing ◽  
Fabrication Process ◽  
Eggshell Membrane ◽  
Templated Synthesis ◽  
Free Glucose

The fabrication process of the nonenzyme glucose sensing based Cu2+–Cu+/biochar.

An enzymatic glucose sensor based on glucose oxidase immobilized cup-stacked carbon nanofilaments

Carbon ◽  
2021 ◽  
Vol 178 ◽  
pp. 822
Author(s):  
Shingo Akiyama ◽  
Momoko Toyoda ◽  
Kiyoharu Nakagawa
Keyword(s):  
Glucose Oxidase ◽  
Glucose Sensor

A long-term stable paper-based glucose sensor using a glucose oxidase-loaded, Mn2BPMP-conjugated nanocarrier with a smartphone readout

Nanoscale ◽  
2021 ◽  
Author(s):  
Soyeon Yoo ◽  
Kiyoon Min ◽  
Giyoong Tae ◽  
Min Su Han
Keyword(s):  
Glucose Oxidase ◽  
Glucose Sensor ◽  
Sensor System ◽  
Term Stability ◽  
Long Term Stability

A paper-type sensor system was devised using an enzyme-loaded, artificial peroxidase-conjugated nanocarrier to maintain long-term stability with smartphone readout.

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