scholarly journals Push–pull pyropheophorbides for nonlinear optical imaging

2017 ◽  
Vol 15 (4) ◽  
pp. 947-956 ◽  
Author(s):  
Anjul Khadria ◽  
Yovan de Coene ◽  
Przemyslaw Gawel ◽  
Cécile Roche ◽  
Koen Clays ◽  
...  
Keyword(s):  
Harmonic Generation ◽  
Lipid Membranes ◽  
Nonlinear Optical ◽  
Second Harmonic ◽  
Two Photon ◽  
Donor And Acceptor ◽  
Nonlinear Optical Imaging ◽  
Pyropheophorbide A ◽  
Electron Donor And Acceptor ◽  
Derivatives Of

We have synthesized derivatives of pyropheophorbide-a with electron donor and acceptor groups which localize in lipid membranes and can be used for multi-photon imaging via second harmonic generation and two-photon excited fluorescence.

scholarly journals Nonlinear optical imaging of single plasmonic nanoparticles with 30 nm resolution

2015 ◽  
Vol 17 (33) ◽  
pp. 21288-21293 ◽  
Author(s):  
Anke Horneber ◽  
Kai Braun ◽  
Jan Rogalski ◽  
Paul Leiderer ◽  
Alfred J. Meixner ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Optical Imaging ◽  
Optical Microscopy ◽  
Harmonic Generation ◽  
Nonlinear Optical ◽  
Second Harmonic ◽  
Near Field ◽  
Plasmonic Nanoparticles ◽  
Two Photon ◽  
Nonlinear Optical Imaging

Femtosecond-scanning near-field optical microscopy resolves the location-correlated second harmonic generation and two-photon photoluminescence from single nanoparticles with 30 nm resolution.

Intermolecular Coupling Enhancement of the Molecular Hyperpolarizability in Multi-chromophoric Dipolar Dendrons

1999 ◽  
Vol 598 ◽  
Author(s):  
Shiyoshi Yokoyama ◽  
Akira Otomo ◽  
Shinro Mashiko
Keyword(s):  
Nonlinear Optical ◽  
Rayleigh Scattering ◽  
Second Harmonic ◽  
Second Order ◽  
Donor And Acceptor ◽  
Nonlinear Optical Chromophore ◽  
Scattering Technique ◽  
Molecular Hyperpolarizability ◽  
Azobenzene Chromophore ◽  
Electron Donor And Acceptor

ABSTRACTNonlinear optical dendritic macromolecules, called “azobenzene dendrons”, having a branching structure modified with a second-order nonlinear optical chromophore has been synthesized. An electron donor and acceptor azobenzene chromophore having a large molecular hyperpolarizability of β0=150E-30 esu, was chosen. The electronic structure of synthesized dendrons, which were expected to become dipolar due to their intermolecular attractive interaction, was proven by second-order nonlienar optical properties. The molecular hyperpolarizabilities of azobenzene dendrons were measured by the hypr-Rayleigh scattering technique. The highest molecular hyperpoalrizability was found to be 3,010E-30 esu for an azobenzene dendron having 15 chromophoric unit, where each chromophore coherently contribute to the second harmonic generation. This level of the molecular hyperpolarizability was much larger than that for a monomeric azobenzene chromophore.

scholarly journals Label-free characterization of collagen fibers in cancerous esophagus tissues using ratiometric nonlinear optical microscopy

2020 ◽  
Vol 245 (14) ◽  
pp. 1213-1221
Author(s):  
Wei-Chung Chen ◽  
Yu-Jen Chen ◽  
Shih-Ting Lin ◽  
Wei-Han Hung ◽  
Ming-Che Chan ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Esophageal Cancer ◽  
Esophageal Squamous Cell Carcinoma ◽  
Optical Microscopy ◽  
Harmonic Generation ◽  
Nonlinear Optical ◽  
Second Harmonic ◽  
Collagen Fibers ◽  
Nonlinear Optical Microscopy ◽  
Two Photon

A quantitative analytical method to discriminate among the various types of cancerous esophagus tissue is essential for accurate cancer staging. This paper reports on the use of ratiometric nonlinear optical microscopy to reveal the ratio of two-photon excited fluorescence (TPEF) to second harmonic generation (SHG) and forward to backward (F/B) SHG from single collagen fibers only in submucosa of esophageal squamous cell carcinoma. This makes it possible to accurately differentiate among the four stages of esophageal cancer, providing results that are in good agreement with histopathology. Furthermore, it is confirmed by polarization-dependent SHG that the varied SHG response in esophageal cancer tissues is mainly from the shrinkage in diameter of collagen fibers instead of the collagen triple helixes altered by cancer cells. Based on the results of TPEF/SHG and F/B SHG ratio, they can cooperatively improve the precision of diagnostics on esophageal cancer and could be transferred to other types of cancer diseases with changed collagen fibers. Impact statement The issue of classifying esophageal cancer at various developmental stages is crucial for determining the optimized treatment protocol for the patients, as well as the prognosis. Precision improvement in staging esophageal cancer keeps seeking quantitative and analytical imaging methods that could augment histopathological techniques. In this work, we used nonlinear optical microscopy for ratiometric analysis on the intrinsic signal of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) from single collagen fibers only in submucosa of esophageal squamous cell carcinoma (ESCC). The blind tests of TPEF/SHG and forward (F)/backward (B) SHG were demonstrated to compare with the histology conclusion. The discussion of sensitivity and specificity was provided via statistical comparison between the four stages of esophageal cancer. To the best of our knowledge, this is the first study of using these two ratios in combination for staging ESCC.

A series of multifunctional coordination polymers based on terpyridine and zinc halide: second-harmonic generation and two-photon absorption properties and intracellular imaging

2017 ◽  
Vol 5 (27) ◽  
pp. 5458-5463 ◽  
Author(s):  
Jian Su ◽  
Jun Zhang ◽  
Xiaohe Tian ◽  
Meng Zhao ◽  
Tao Song ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Coordination Polymers ◽  
Nonlinear Optical ◽  
Second Harmonic ◽  
Photon Absorption ◽  
Absorption Properties ◽  
Intracellular Imaging ◽  
Two Photon Absorption ◽  
Two Photon

Nonlinear optical CPs with good one- and two-photon excited fluorescence and SHG properties are presented.

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