Site-specific covalent capture of human O6-alkylguanine-DNA-alkyltransferase using single-stranded intrastrand cross-linked DNA

2017 ◽  
Vol 15 (1) ◽  
pp. 189-196 ◽  
Author(s):  
D. K. O'Flaherty ◽  
C. J. Wilds
Keyword(s):  
Efficient Method ◽  
Site Specific ◽  
Dna Alkyltransferase ◽  
Protein Covalent

An efficient method to generate site-specific DNA–protein covalent complexes is described using intrastrand cross-linked DNA.

Site-Specific, Covalent Labeling of Recombinant Antibody Fragments via Fusion to an Engineered Version of 6-O-Alkylguanine DNA Alkyltransferase

2009 ◽  
Vol 20 (5) ◽  
pp. 1010-1015 ◽  
Author(s):  
Florian Kampmeier ◽  
Markus Ribbert ◽  
Thomas Nachreiner ◽  
Sofia Dembski ◽  
Florent Beaufils ◽  
...  
Keyword(s):  
Recombinant Antibody ◽  
Antibody Fragments ◽  
Covalent Labeling ◽  
Site Specific ◽  
Recombinant Antibody Fragments ◽  
Dna Alkyltransferase

A diazirine-based photoaffinity probe for facile and efficient aptamer–protein covalent conjugation

2014 ◽  
Vol 50 (38) ◽  
pp. 4891-4894 ◽  
Author(s):  
Huimin Zhang ◽  
Yanling Song ◽  
Yuan Zou ◽  
Yun Ge ◽  
Yuan An ◽  
...  
Keyword(s):  
Site Specific ◽  
Protein Covalent ◽  
Specific Labelling

A photo-reactive functional labelling reagent was designed and synthesized for site-specific labelling of oligonucleotide with the diazirine moiety for facile and efficient photo-crosslinking of the resulting oligonucleotide with its interacting partner for a variety of applications.

scholarly journals Efficient System for Directed Integration into theLactobacillus acidophilus and Lactobacillus gasseri Chromosomes via Homologous Recombination

2001 ◽  
Vol 67 (9) ◽  
pp. 4361-4364 ◽  
Author(s):  
W. M. Russell ◽  
T. R. Klaenhammer
Keyword(s):  
Homologous Recombination ◽  
Efficient Method ◽  
Lactobacillus Acidophilus ◽  
Gene Encoding ◽  
Efficient System ◽  
Site Specific ◽  
Lactobacillus Gasseri ◽  
Integration Strategy ◽  
Insertional Inactivation ◽  
Simultaneous Use

ABSTRACT An efficient method is described for the generation of site-specific chromosomal integrations in Lactobacillus acidophilus and Lactobacillus gasseri. The strategy is an adaptation of the lactococcal pORI system (K. Leenhouts, G. Venema, and J. Kok, Methods Cell Sci. 20:35–50, 1998) and relies on the simultaneous use of two plasmids. The functionality of the integration strategy was demonstated by the insertional inactivation of the Lactobacillus acidophilus NCFMlacL gene encoding β-galactosidase and of theLactobacillus gasseri ADH gusA gene encoding β-glucuronidase.

scholarly journals Use of a yeast site-specific recombinase to produce female germline chimeras in Drosophila.

Genetics ◽  
1992 ◽  
Vol 131 (3) ◽  
pp. 643-653 ◽  
Author(s):  
T B Chou ◽  
N Perrimon
Keyword(s):  
Heat Shock ◽  
Efficient Method ◽  
Mitotic Recombination ◽  
Site Specific ◽  
X Ray ◽  
Flp Recombinase ◽  
Lethal Mutations ◽  
Recombination System ◽  
Dominant Female ◽  
Female Germline

Abstract We describe an efficient method for generating female germline mosaics by inducing site-specific homologous mitotic recombination with a yeast recombinase (FLP) which is driven by a heat shock promoter. These germline mosaics are produced in flies heterozygous for the agametic, germline-dependent, dominant female sterile (DFS) mutation ovoD1, where only flies possessing germline clones are able to lay eggs. This method, the "FLP-DFS" technique, is very efficient because more than 90% of females with germline clones can be recovered. We show that this heat-inducible, site-specific mitotic recombination system does not affect viability and that the germline clones recovered are physiologically the same as those created by X-ray induced mitotic recombination. We describe the parameters of FLP-recombinase induced germline mitotic recombination and the use of the "FLP-DFS" technique to analyze the maternal effect of X-linked zygotic lethal mutations.

Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells

genesis ◽  
2006 ◽  
Vol 44 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Caroline Beard ◽  
Konrad Hochedlinger ◽  
Kathrin Plath ◽  
Anton Wutz ◽  
Rudolf Jaenisch
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Efficient Method ◽  
Embryonic Stem ◽  
Single Copy ◽  
Site Specific ◽  
Site Specific Integration
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