scholarly journals Genetic incorporation of 1,2-aminothiol functionality for site-specific protein modification via thiazolidine formation

2016 ◽  
Vol 14 (23) ◽  
pp. 5282-5285 ◽  
Author(s):  
Xiaobao Bi ◽  
Kalyan Kumar Pasunooti ◽  
Ahmad Hussen Tareq ◽  
John Takyi-Williams ◽  
Chuan-Fa Liu
Keyword(s):  
Protein Modification ◽  
Specific Protein ◽  
Site Specific ◽  
Amber Codon ◽  
Amber Codon Suppression ◽  
Genetic Incorporation

Thiazolidine ligation was used to modify site-specifically proteins harbouring a 1,2-aminothiol moiety introduced by amber codon suppression technology.

Systematic Engineering of a Protein Nanocage for High-Yield, Site-Specific Modification

2018 ◽  
Author(s):  
Daniel D. Brauer ◽  
Emily C. Hartman ◽  
Daniel L.V. Bader ◽  
Zoe N. Merz ◽  
Danielle Tullman-Ercek ◽  
...  
Keyword(s):  
Small Molecules ◽  
Protein Modification ◽  
Positive Charge ◽  
Specific Protein ◽  
High Yield ◽  
Site Specific ◽  
Protein Cage ◽  
Protein Carriers ◽  
Site Selective ◽  
Targeted Library

<div> <p>Site-specific protein modification is a widely-used strategy to attach drugs, imaging agents, or other useful small molecules to protein carriers. N-terminal modification is particularly useful as a high-yielding, site-selective modification strategy that can be compatible with a wide array of proteins. However, this modification strategy is incompatible with proteins with buried or sterically-hindered N termini, such as virus-like particles like the well-studied MS2 bacteriophage coat protein. To assess VLPs with improved compatibility with these techniques, we generated a targeted library based on the MS2-derived protein cage with N-terminal proline residues followed by three variable positions. We subjected the library to assembly, heat, and chemical selections, and we identified variants that were modified in high yield with no reduction in thermostability. Positive charge adjacent to the native N terminus is surprisingly beneficial for successful extension, and over 50% of the highest performing variants contained positive charge at this position. Taken together, these studies described nonintuitive design rules governing N-terminal extensions and identified successful extensions with high modification potential.</p> </div>

Further Studies on the Site-Specific Protein Modification by Microbial Transglutaminase

2001 ◽  
Vol 12 (5) ◽  
pp. 701-710 ◽  
Author(s):  
Haruya Sato ◽  
Eiko Hayashi ◽  
Naoyuki Yamada ◽  
Masanobu Yatagai ◽  
Yoshiyuki Takahara
Keyword(s):  
Protein Modification ◽  
Specific Protein ◽  
Microbial Transglutaminase ◽  
Site Specific

scholarly journals Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

2015 ◽  
Vol 10 (3) ◽  
pp. 508-516 ◽  
Author(s):  
Martin D Witte ◽  
Tongfei Wu ◽  
Carla P Guimaraes ◽  
Christopher S Theile ◽  
Annet E M Blom ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Protein Modification ◽  
Specific Protein ◽  
Site Specific ◽  
Flow Systems

Site-Specific Protein Modification through CuI-Catalyzed 1,2,3-Triazole Formation and Its Implementation in Protein Microarray Fabrication

2006 ◽  
Vol 45 (26) ◽  
pp. 4286-4290 ◽  
Author(s):  
Po-Chiao Lin ◽  
Shau-Hua Ueng ◽  
Mei-Chun Tseng ◽  
Jia-Ling Ko ◽  
Kuo-Ting Huang ◽  
...  
Keyword(s):  
Protein Modification ◽  
Protein Microarray ◽  
Specific Protein ◽  
Site Specific ◽  
Microarray Fabrication

Site-Specific Protein Modification Using a Ketone Handle

1996 ◽  
Vol 118 (34) ◽  
pp. 8150-8151 ◽  
Author(s):  
Virginia W. Cornish ◽  
Klaus M. Hahn ◽  
Peter G. Schultz
Keyword(s):  
Protein Modification ◽  
Specific Protein ◽  
Site Specific

Site-Specific Protein Modification through CuI-Catalyzed 1,2,3-Triazole Formation and Its Implementation in Protein Microarray Fabrication

2006 ◽  
Vol 118 (26) ◽  
pp. 4392-4396 ◽  
Author(s):  
Po-Chiao Lin ◽  
Shau-Hua Ueng ◽  
Mei-Chun Tseng ◽  
Jia-Ling Ko ◽  
Kuo-Ting Huang ◽  
...  
Keyword(s):  
Protein Modification ◽  
Protein Microarray ◽  
Specific Protein ◽  
Site Specific ◽  
Microarray Fabrication

Local Unfolding Is Required for the Site-Specific Protein Modification by Transglutaminase

Biochemistry ◽  
2012 ◽  
Vol 51 (43) ◽  
pp. 8679-8689 ◽  
Author(s):  
Barbara Spolaore ◽  
Samanta Raboni ◽  
Amparo Ramos Molina ◽  
Abhijeet Satwekar ◽  
Nunzio Damiano ◽  
...  
Keyword(s):  
Protein Modification ◽  
Specific Protein ◽  
Site Specific ◽  
Local Unfolding
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